FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_71.34100000004c9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_71.34100000004c9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569264
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC25690.4512844655555243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG3050.036.88517436
CGAGACT2950.036.61009623
TCGTATG3250.035.30762540
TATGCCG3350.034.92530443
ACGAGAC3350.034.92530422
GTATCTC2950.034.32196435
CTCGTAT3350.034.25366239
CACGAGA3500.034.0713621
TGCCGTC3450.033.9129845
GACTCAA3100.033.3870326
ACTCAAC3150.032.8570827
ATGCCGT3650.032.05473344
CGTATGC3650.031.43829541
TCTCGTA3700.031.01345338
GCCCACG4000.030.3749418
GGTATCA4300.030.3514441
CCACGAG3950.030.18981620
CCGAGCC3900.029.99994314
ACGTACC300.005145150229.999947
CGCGTGG300.005145150229.9999412
Produced by FastQC (version 0.11.2)