FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_70.34100000004c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_70.34100000004c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656154
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1001.8189894E-1229.24945443
TCGTATG1200.026.24951240
CTCGTAT1301.8189894E-1224.23031839
TGCCGTC1252.910383E-1123.39956545
CGTATGC1353.6379788E-1223.332941
GGTATCA5700.023.296141
GTATCAA11000.022.0972331
CGAAGTT1950.021.9243415
CGAGCCC1459.094947E-1221.72373415
ATGCCGT1401.364242E-1020.89246744
TCTCCGA6650.020.63871245
TTAACGA1109.969699E-820.4541654
ACGCCTA807.557766E-519.68713421
CGTTAGG600.006496841618.7553671
CTATTAT600.006496841618.7553671
TATCTCG1454.7621143E-918.62034236
TCTCGTA1709.094947E-1118.52906638
CATCTCC22250.018.50527245
CCTTACG750.001109671717.9996642
CGCCTAA901.864403E-417.49967622
Produced by FastQC (version 0.11.2)