FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_69.34100000004c72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_69.34100000004c72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617982
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG250.002106691235.99883313
CTTATAT352.8194132E-432.141823
CGCCCGT406.1640586E-428.1240910
GGTATCA4700.026.337461
GTATCAA10100.024.512091
CCGAACT500.0022638622.4992714
TACACTA500.0022638622.4992715
GTCTAGT654.2268547E-420.7752821
AACGACG654.2361004E-420.76855915
CCCGAAC654.2361004E-420.7685593
CCCGTGC550.003935248620.4538828
TATGCCG807.557655E-519.68686343
TCTCCGA6450.019.5342545
GGCAATC706.9799885E-419.28509114
ATATGAG600.006508383418.7493933
TAAATAC600.006508383418.7493933
GCCCGTC600.006508383418.74939311
TCGTATG851.2035962E-418.52881240
TATCAAC13250.018.1692242
CTAACCC750.001109674717.9994163
Produced by FastQC (version 0.11.2)