FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_68.34100000004c65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_68.34100000004c65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10295
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC230922.42836328314716No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG6045.8669256920835355No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT2852.768334142787761No Hit
GTTATGAAGCCTAGAACGATAAAGAGGCATATCGTACAAGGATGCAAAAAC410.39825157843613407No Hit
AAATCATGAGCATCAAAACGCTCACCTTTCAGCGCAAGAAATACATCACCA350.3399708596406022No Hit
GCAGATGAAAATTCAAATTGCTGCGATTTAACTGTTTCTAGTCGCTGTTTT320.3108305002428363No Hit
CACTTACGTGAAGGGACTGAATGTGCTGATCAACGTGTACTTGAAGCCGTT310.30111704711024767No Hit
TAAACGCCATTCAGGAAAATACATCATGAAATCCCTGTCCTTACTCATCAC270.26226323457989315No Hit
GGGCTATTTTTGGCACACTGGGCTTTGGCTTTTGGTTCTTTTTCTACACCT260.2525497814473045No Hit
GCAGTAATCAACCAACCCCATGCAAACATTGGCATTTTCATCAATGTCATG250.24283632831471588No Hit
AGACAAGACTGGATCTGATCAAGCGCTTCTATGATGCGGTGTCTACTTTCA240.23312287518212726No Hit
TACACATACCGCAGATGGCTTGAAAGTGGCGCTTGAACACCGTAATCCACA240.23312287518212726No Hit
TATCACGACTCGTTCGACCCCATTCGCGCAGTGCGAACAAAAGACTTCAGT230.22340942204953862No Hit
ATGCGAGACCGTTTCGGAGCCTTAACCTAATGTTGAACCGGGGTAATTAGG220.21369596891694995No Hit
GTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTT220.21369596891694995No Hit
GTAGGCACACGCTTGTTCGGTAAAGTATCTAACTTGACTGACTACGGTGCT220.21369596891694995No Hit
CTTCAGTAAATCGGGCACGCACCGACCAGCCACAATCAGAAACCTGTCTCT210.20398251578436136No Hit
AAAAAGCATTGTGCTTGAGTTACAACAACTGATCTGGCCTAACTATGCAGA210.20398251578436136No Hit
CTATAAAGACACAAGCACACGTATGTTTATTGCAGCACTATTCACAATAGC210.20398251578436136No Hit
GACCTTAACGGGGGCTAAAAACATTCAAGCGATTAACTCAGATTGCTTGGT200.19426906265177268No Hit
GGCCAGTACCACCGCAAGTTCATGCTGCAGCTTATATCGAGCCAGCTTGAT200.19426906265177268No Hit
CGCCAGATCAGTTTTTCCACATACAGCTTTTCCGTGATGTCGCGCGCAATT200.19426906265177268No Hit
CTTCCATCTCCCCAGCCAACTCTTACGTATCTTTTTAGGGTCATCTTAAGC190.18455560951918407No Hit
GCGCAGGACCCCTTATTTAGACCAGTGAGCTGTTACGCTTTCTTTAAAGGA180.17484215638659545No Hit
GGCTAAGCCGGTAGCCGATCCTTAGTCCCTCTTCTAGATTTCTTGAAGAGG180.17484215638659545No Hit
CATACAGCCAATCCGTGCTCTCATCTTGCATTGGAATAGATAGCGTTAGGC180.17484215638659545No Hit
GTGGTGAATTCGCCATGAAAAGTATTGCTTATCGCGCAAAGGGTTTTATGT160.15541525012141816No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC160.15541525012141816No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC160.15541525012141816No Hit
GAGCGCGTAGCTGAAACATGCCGATTGATGATTGCAAACGGATTCCTCGTT160.15541525012141816No Hit
AGTAGGATATAGTGATCCGATGATTCAGAATGGAATGATCATCGCTCAAGA160.15541525012141816No Hit
CGCCTGTAATCCCAGCTACTCAGGAGGCTGAGACAGGAGAATAACTTGAAC160.15541525012141816No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC160.15541525012141816No Hit
GACTGGGTTTGAGGTGAAAGATGTGTGTGTATTTGGCAGCAGTTAAAGGGC150.1457017969888295No Hit
ATCAATCAGTGCGGAAATCTCTGGTGGAAGTTTTTCGACAGAATCAAAACC150.1457017969888295No Hit
TGTTTGGCCTGATAAATGCGGATAGCCTCTTGCGTTTTAAGGCCAGGAAAA150.1457017969888295No Hit
GCTATGTTGGGCAAACCCCTTCCTCCTAAGTTGTTCTGTGGCACTCTCAAA150.1457017969888295No Hit
CCGCTGTACCGCCGGATTATTTTGAGAGTTTTAACCGAAAACGTGAGAACT140.1359883438562409No Hit
GGTCTATGCAGTGCAATCGATTATCAGTGGCCCGCTTGATCGCGCCAAACT140.1359883438562409No Hit
CACCTGGCAAAATGACGGCACAATAGGCTCACAGCCTGTCTCTTATACACA140.1359883438562409No Hit
GGGCTATTGTGTATCTTAACCAGCATCTCAATCAAGCTATCTCAAACCGTA140.1359883438562409No Hit
CGCCTGCAGAAAGGACTAAACCATCGATTTTTTTCCCTTCCAAATGGCCCA140.1359883438562409No Hit
TGCCAATCCCGATGTGATCGTTACCGATATCATGATGCCCTATGCCTCCGG140.1359883438562409No Hit
CTTCTGTCCAACCCGGAATAACCTGGTTAAGTCCAAATGAAATAGGTTGAC140.1359883438562409No Hit
CCTGTTAGTTCTAAGGCAGTCTCTAAATTTCCCAAGAGGTAAAGCAGAAAG130.12627489072365225No Hit
GCCACAGGACGTAAGCAAGCATCAAGCATTTGCATTCCACTACAATTAACC130.12627489072365225No Hit
GTGAAATAGAACCTGAAACCGTACGCCTACAAGCGGTCGGAGCACCAATAA130.12627489072365225No Hit
GATAAGGATTGGCTGATAGAAGAATGCAAGAAAAGATTTATCGTCAGTGGC130.12627489072365225No Hit
GGTTTAATGGCAAGATCTTTCTTTTCACCGTAAACGAAGTTAGGTCCCATA130.12627489072365225No Hit
CTCAGAGACCACGGCTGGAGTAATAAGCTTATAGTTCGGAACCTCCTTACA130.12627489072365225No Hit
GGTTAAAAACTGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCCAATCAAA130.12627489072365225No Hit
GAGCTGATGACGAGTTGCCTTTAGGCGACGAAGTGGTTGATGCCATGCTTC130.12627489072365225No Hit
AGGCTACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATATAG130.12627489072365225No Hit
CTCGTAAGTAGCTATTGAACAAAAGAATGGTAGATAAGGGTAGCGATTCAT130.12627489072365225No Hit
CACCCAGACATTGCTGATCACGTTCATGATGATGACCAGCAGGCCCATGGC130.12627489072365225No Hit
CAGCAGCAAGAATTAGCCCGTTTTGGTTAATTCTTGCTGATACCAGCCGAT130.12627489072365225No Hit
CCTGTACCCAAGCCAAGATAAATCAATCCATTAGCTGGCTTGCCTGCTAAA130.12627489072365225No Hit
GCTAAGCATGTGAGCTTGTCTTGGGAGTGTTTTTGATGCTTCTGGGGTGGG130.12627489072365225No Hit
ATACAGCAGCGTGATATGGATGTCGCTCAATTGGCGGCGATTTTGCAAACA130.12627489072365225No Hit
GCTGCCAACGGCGCAGTGTCACCGGTACGTAAATTTGTACCAGACGAAATT120.11656143759106363No Hit
CCCACATCCTTTTCCACTTAGCACACCCTTAGGGGCCTGTCTCTTATACAC120.11656143759106363No Hit
GCTTCAGCCTCATTGAAAAGTGCAAGAGGAAACAATCAGAAGCAGGTTATA120.11656143759106363No Hit
TTCATGGTTTGTATTCACACTGAAAATCCCGTACTCTGCGTTGATACCTGT120.11656143759106363No Hit
GATGTGTATAAGAGACAGACGCGTACCAGTTTGCCCTGTGGTTGCTGTAGT120.11656143759106363No Hit
CCTTTTGCTTTTTCTCCATGTCACGGTACTGGCCCTGGTACTGTCTCTTAT120.11656143759106363No Hit
ATGCATGAGTTACTTGATGAAGAGGTAGATCTGATGATCAAGGCCTGTCTC120.11656143759106363No Hit
CTCCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAA120.11656143759106363No Hit
CCCTCGTGCAATCAGTAAATAATAGGGACTCTTACGTTTACATAAACCCAC120.11656143759106363No Hit
CAGACATATTCGGTGTACCGTTGGACTCACCAGTTGATGTACTGTCTCTTA120.11656143759106363No Hit
ACATGTTGTGGTTGACTCCTATTATTCCTTGTCCAAACCACAATACGTCAT120.11656143759106363No Hit
AAGGAGCCGATGAGGTACCTACAGTTGTCGGGTCGAAAGACAACGAAGCAG120.11656143759106363No Hit
CAAAATCCCACTGCTCAAGTTCTTCATCAAGCACGAGCTGATCTACGTGCC120.11656143759106363No Hit
GTATAAAGTTCACTCGGGCGGGAAGTTTCCCGCATCAGCTTCATCCATGCA120.11656143759106363No Hit
GGAATCTGAGTATTGTAGCAACCAGCTTCAATCATGCCGTGATAACAATGC120.11656143759106363No Hit
AGCGGGTGCCGCACCATTCAATGTTAGATTTGAGATTAAGCTTGATGGGAA120.11656143759106363No Hit
CCGGAAACAAGGCACGAGAAGCAGCCCAAGTGATATTTTGCACTGGTAACA120.11656143759106363No Hit
CACTACATCCGGGCAGTTATGGCAGCTGAGCGAGATGTAGGTCTCAAAATG120.11656143759106363No Hit
GACTCGACTAGTGAGCTATTACGCTTTCTTTAAAGGGTGGCTGCTTCTAAG110.10684798445847497No Hit
GATCAAATCATCAAACTTGGCAATACCCATACTTGCCATTAACTCACGCAC110.10684798445847497No Hit
ATCCAGATCAAATACCTGCACGGTCAGGAATACTCGGTGGACCAGGCCTGT110.10684798445847497No Hit
GCGAGATGTGGTCAACCCAAAGTCCAGGGAATGTGGTTGCATGTCGTAATA110.10684798445847497No Hit
CACTGAGTAGAAGCTCCGAAAGATGATGCCGTGGAGGGCTGTCTCTTATAC110.10684798445847497No Hit
TTCCTGTACCACCTAGTATCGTTTTAATCGATGGGGGGACGCAGTAGGATA110.10684798445847497No Hit
ATAAAGGTACTGATCAAGGTTTTGAACGAGTTTCTGGCCTCAACCAGGCTA110.10684798445847497No Hit
GTTGGAACTGGCTTGCAAATATTTCACCATAAAGCGTGTCAGTGCGCCAAA110.10684798445847497No Hit
AGCCCCTTTGTTTTGGCCAATTTCTCCCATTTACAATGGGTGTAGTAGCCA110.10684798445847497No Hit
GATGTCTTTTCCTCGAGTTACTTAGATGTTTCAGTTCACTCGGTTCGCCTC110.10684798445847497No Hit
CTTCTATCCCATCAATCCTCAGAAGATTGGCTTCACCCCCTTGTGGAAAAA110.10684798445847497No Hit
AAACAATTTACCGTGAGTCTGTTTTCGTTAATTCGCTGACAGATCAAGACC110.10684798445847497No Hit
CCTCAAAGATGGACAGTTTGAGGCAAGGATGACAAGTTTTGCTTTGCCTTG110.10684798445847497No Hit
CATACCTATTGTCCCAGCTACTCAAGAGGCTGAAGCAGGAGGATCTGTTGC110.10684798445847497No Hit
GAATGAACGACTCGAAGAGCATCGTCAAAATTTAGCATTTGCGCATGAAAC110.10684798445847497No Hit
GGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAA110.10684798445847497No Hit
GCTATAATAGAGCGAGATATCCACGGAGACGTGTTGAAAGCGGGGTATAGT110.10684798445847497No Hit
CTACTTTGGTGCGTTTACCTTCAAATGCGAATGGCTTGGTCACCACTGCGA110.10684798445847497No Hit
ATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATCCCGTACTCTGCGTTGA110.10684798445847497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2650.045.045
ATGCCGT3100.038.46774344
GTATGCC3800.031.38157842
TATGCCG3800.031.38157843
CGTATGC3850.030.97402441
ATCTCGT3800.030.78947337
TCTCGTA3800.030.78947338
TCGTATG3800.030.78947340
CTCGTAT3800.030.78947339
GTATCTC3700.030.40540335
TATCTCG3800.030.19736936
CTGTATC3800.030.19736933
TGTATCT3750.030.034
CGAGACT4000.029.812523
ACTGTAT3850.029.80519332
CAACTGT3850.029.80519330
TCAACTG3850.029.80519329
ACTCAAC3850.029.80519327
GACTCAA3850.029.80519326
CTCAACT3850.029.80519328
Produced by FastQC (version 0.11.2)