##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_68.34100000004c65.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10295 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.01486158329286 38.0 38.0 38.0 32.0 38.0 2 36.11209324915007 38.0 38.0 38.0 32.0 38.0 3 36.05905779504614 38.0 32.0 38.0 32.0 38.0 4 36.21359883438562 38.0 38.0 38.0 32.0 38.0 5 36.31238465274405 38.0 38.0 38.0 32.0 38.0 6 38.2656629431763 40.0 38.0 40.0 38.0 40.0 7 38.04876153472559 40.0 38.0 40.0 32.0 40.0 8 37.95764934434192 40.0 38.0 40.0 32.0 40.0 9 38.05041282175814 40.0 38.0 40.0 38.0 40.0 10 37.89198640116562 40.0 38.0 40.0 32.0 40.0 11 38.01699854298203 40.0 38.0 40.0 32.0 40.0 12 37.8382710053424 40.0 38.0 40.0 32.0 40.0 13 38.00194269062652 40.0 38.0 40.0 32.0 40.0 14 37.86080621661001 40.0 38.0 40.0 32.0 40.0 15 37.84662457503642 40.0 38.0 40.0 32.0 40.0 16 37.64662457503643 40.0 38.0 40.0 32.0 40.0 17 37.630014570179696 40.0 38.0 40.0 32.0 40.0 18 37.49111219038368 40.0 38.0 40.0 32.0 40.0 19 37.674113647401654 40.0 38.0 40.0 32.0 40.0 20 37.64507042253521 40.0 38.0 40.0 32.0 40.0 21 37.72209810587664 40.0 38.0 40.0 32.0 40.0 22 37.75114133074308 40.0 38.0 40.0 32.0 40.0 23 37.77765905779505 40.0 38.0 40.0 32.0 40.0 24 37.67246236036911 40.0 38.0 40.0 32.0 40.0 25 37.53715395823215 40.0 38.0 40.0 32.0 40.0 26 37.539485186983974 40.0 38.0 40.0 32.0 40.0 27 37.26070908207868 40.0 38.0 40.0 32.0 40.0 28 37.47226809130646 40.0 38.0 40.0 32.0 40.0 29 37.31792132102962 40.0 38.0 40.0 32.0 40.0 30 37.48994657600777 40.0 38.0 40.0 32.0 40.0 31 37.376979116075766 40.0 38.0 40.0 32.0 40.0 32 37.52413793103448 40.0 38.0 40.0 32.0 40.0 33 37.561146187469646 40.0 38.0 40.0 32.0 40.0 34 37.57464788732394 40.0 38.0 40.0 32.0 40.0 35 37.55220981058766 40.0 38.0 40.0 32.0 40.0 36 37.648761534725594 40.0 38.0 40.0 32.0 40.0 37 37.35036425449247 40.0 38.0 40.0 32.0 40.0 38 37.55036425449247 40.0 38.0 40.0 32.0 40.0 39 37.186692569208354 38.0 38.0 40.0 32.0 40.0 40 37.150072850898496 38.0 38.0 40.0 32.0 40.0 41 37.29431762991744 40.0 38.0 40.0 32.0 40.0 42 37.229820301117044 40.0 38.0 40.0 32.0 40.0 43 37.54210781932977 40.0 38.0 40.0 32.0 40.0 44 37.323652258377855 40.0 38.0 40.0 32.0 40.0 45 37.57872753763963 40.0 38.0 40.0 32.0 40.0 46 37.28033025740651 40.0 38.0 40.0 32.0 40.0 47 37.36668285575522 40.0 38.0 40.0 32.0 40.0 48 37.21826129188927 40.0 38.0 40.0 32.0 40.0 49 37.13530840213696 40.0 38.0 40.0 32.0 40.0 50 37.054006799417195 38.0 38.0 40.0 32.0 40.0 51 36.364934434191355 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.4369065364191584 2201 2 1.4148815890797763 2201 3 -0.7037266219977312 2201 4 0.9543860518995899 2201 5 0.4264564748788615 2201 6 1.1119020071050585 2201 7 1.6642221375653108 2201 8 1.293784099631985 2201 9 1.4586376655733773 2201 10 1.4746959149623393 2201 11 1.5466930132722112 2201 12 0.7586956338268536 2201 13 0.30580592290514375 2201 14 1.1571213571134962 2201 15 1.3671951086382492 2201 16 1.8530877797651613 2201 17 1.7850231833010497 2201 18 2.025559002575612 2201 19 1.4005034007664392 2201 20 0.347209736119261 2201 21 0.447106602252461 2201 22 -0.005277321255455547 2201 23 1.0835312422302152 2201 24 -0.7555512280036965 2201 25 -0.1647420884740569 2201 26 0.523915605579802 2201 27 -0.4517538845125699 2201 28 -0.032770917982190895 2201 29 -0.05745938103112991 2201 30 0.37501697642043297 2201 31 0.7824746245514689 2201 32 1.887957173451447 2201 33 1.7733001178978824 2201 34 1.3471958147255805 2201 35 1.8383093069667993 2201 36 1.5277484021074414 2201 37 2.1930434274027064 2201 38 1.5954333055817003 2201 39 1.1736813608862704 2201 40 1.7464027358600873 2201 41 2.0990290356975905 2201 42 0.6696139595671582 2201 43 1.388053780718991 2201 44 2.1391280154770627 2201 45 2.0678761341481504 2201 46 1.9380535533840728 2201 47 1.7354063276603569 2201 48 0.4732294568123052 2201 49 1.4092260707343982 2201 50 0.44335146720861474 2201 51 1.5836365660832357 1101 1 -1.5345220350094095 1101 2 -1.7565469823487945 1101 3 0.005797187526084713 1101 4 0.5401003376138718 1101 5 -0.6878292394068524 1101 6 0.7499972452002979 1101 7 0.026126899470071407 1101 8 0.9318793377272243 1101 9 0.4205424274781393 1101 10 0.2556482959147246 1101 11 0.5085977751769732 1101 12 0.6539337290649527 1101 13 0.3486630657622882 1101 14 -0.8428786428865038 1101 15 1.2910046324477733 1101 16 1.5007068273842066 1101 17 0.03264223092009644 1101 18 0.7207970978137084 1101 19 0.4671700674331092 1101 20 -1.13374264483312 1101 21 -1.2338457786999228 1101 22 0.27567505969692974 1101 23 0.4835312422302138 1101 24 0.10635353390106417 1101 25 -2.9314087551407226 1101 26 -0.8237034420392462 1101 27 0.2196746869160009 1101 28 -0.39943758464885804 1101 29 -0.014602238173985427 1101 30 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1.5007068273842066 2202 17 1.6993088975867678 2202 18 1.3874637644803727 2202 19 -0.46616326590022794 2202 20 1.4662573551668814 2202 21 1.2994875546334157 2202 22 -0.6576582736364074 2202 23 0.41686457556355094 2202 24 0.8396868672343984 2202 25 1.8019245781926116 2202 26 1.5096298912940895 2202 27 2.086341353582668 2202 28 0.8672290820178077 2202 29 0.2520644284926803 2202 30 1.2464455478489995 2202 31 2.034855576932422 2202 32 1.7831952686895463 2202 33 1.8209191655169334 2202 34 1.813862481392249 2202 35 1.3525950212525117 2202 36 0.5753674497264925 2202 37 0.021614855974135594 2202 38 0.9763856865340799 2202 39 1.668919456124371 2202 40 -1.3774067879494325 2202 41 0.5942671309356911 2202 42 0.14104253099572617 2202 43 1.445196637861848 2202 44 -0.2989672226181739 2202 45 0.2964475627195782 2202 46 1.3190059343364524 2202 47 1.9354063276603597 2202 48 0.4970389806218236 2202 49 1.3663689278772537 2202 50 0.8862086100657578 2202 51 0.3979222803689524 1102 1 0.15119225070487374 1102 2 0.12916730336549165 1102 3 -0.27515519342630057 1102 4 0.4543860518995899 1102 5 -0.9306863822639997 1102 6 0.11190200710505849 1102 7 0.3785078518510261 1102 8 -0.06335875751087627 1102 9 0.6014948084305232 1102 10 -2.6681612278947995 1102 11 0.1895501561293571 1102 12 -1.4555900804588617 1102 13 -1.9084797913805644 1102 14 -0.3428786428865038 1102 15 -0.989947748504612 1102 16 0.49594492262230716 1102 17 -1.000691102413235 1102 18 -3.6172981402815267 1102 19 -3.67092517066213 1102 20 -0.08136169245216962 1102 21 -1.4100362548904002 1102 22 -3.076705892684025 1102 23 -0.5593259006269307 1102 24 -2.3984083708608352 1102 25 1.0495436258116584 1102 26 -0.1903701087059133 1102 27 -1.5231824559411464 1102 28 0.610086224874955 1102 29 1.1568263332545854 1102 30 -0.26784016643671293 1102 31 -1.0746682325913923 1102 32 0.3879571734514471 1102 33 1.7733001178978824 1102 34 1.2043386718684346 1102 35 1.2668807355382299 1102 36 -0.11510874074969735 1102 37 -0.4498137154544324 1102 38 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46 1.4523392676697853 1103 47 1.535406327660354 1103 48 1.8970389806218293 1103 49 2.099702261210588 1103 50 2.2862086100657635 1103 51 2.997922280368954 2204 1 0.806654435578821 2204 2 -0.6859587470546771 2204 3 -1.2961635967876433 2204 4 0.38715916114328763 2204 5 -1.8172410041127378 2204 6 -0.14019883323107507 2204 7 -0.9072064338632586 2204 8 -1.6053755642335616 2204 9 -4.112790905855192 2204 10 -1.5169007236931193 2204 11 0.3282056183142288 2204 12 0.6578552976924001 2204 13 -0.6983957577671234 2204 14 0.3335919453487932 2204 15 -1.3638973283365416 2204 16 1.3909029058155795 2204 17 -1.618338161236764 2204 18 0.8658951370293977 2204 19 -0.4818495404100318 2204 20 0.9564534335982486 2204 21 -0.17894381791560932 2204 22 0.1501848636184917 2204 23 -1.7988216989462558 2204 24 0.17302020056773415 2204 25 0.04114026446711705 2204 26 -1.8668406969412104 2204 27 -1.6156194307310585 2204 28 1.408405552606041 2204 29 -2.002837532291629 2204 30 0.8582102537313574 2204 31 0.15642420438339855 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1.1934681851247717 1215 34 0.9354311088432254 1215 35 1.2584773741936885 1215 36 1.5361517634519828 1215 37 1.562791326562369 1215 38 1.8391307845732996 1215 39 1.308135142398875 1215 40 2.0657304669525303 1215 41 1.233482817210195 1215 42 -0.8471927631219174 1215 43 -0.3430386562557999 1215 44 -0.9617123206573908 1215 45 0.496447562719581 1215 46 0.6523392676697881 1215 47 1.6765827982485888 1215 48 1.6539017257198623 1215 49 0.23695716317136828 1215 50 1.2038556688892896 1215 51 1.150863456839538 1212 1 0.19881129832392475 1212 2 0.843453017651207 1212 3 0.27246385419275043 1212 4 1.0734336709472103 1212 5 1.045504093926482 1212 6 1.238886134089185 1212 7 0.87057134391452 1212 8 -0.30145399560610997 1212 9 0.7760979830336936 1212 10 0.6889816292480546 1212 11 0.3085977751769775 1212 12 0.13171150684272703 1212 13 -0.16244804534882462 1212 14 0.8237880237801605 1212 15 -0.1645509231077824 1212 16 1.0118179384953194 1212 17 1.0548644531423221 1212 18 0.5652415422581569 1212 19 -0.8772743770113394 1212 20 0.6884795773891028 1212 21 0.6772653324111886 1212 22 0.9867861708080383 1212 23 0.5279756866746581 1212 24 0.06190908945661988 1212 25 0.05748013374816452 1212 26 -1.579258997594799 1212 27 1.1752302424715566 1212 28 -1.088326473537748 1212 29 -0.0812689048406483 1212 30 0.44644554784900237 1212 31 -0.8206999786231322 1212 32 0.6498619353562134 1212 33 1.154252498850262 1212 34 -0.4861375186077552 1212 35 -0.725182756525264 1212 36 -0.591299216940179 1212 37 -1.0450518106925273 1212 38 0.08749679764519414 1212 39 -0.34219165498674187 1212 40 -2.277406787949431 1212 41 -1.972399535730979 1212 42 -0.03673524678205098 1212 43 -1.3659144732492692 1212 44 -1.8211894448403925 1212 45 -1.003552437280419 1212 46 -0.12543851010799045 1212 47 -0.5979270056729789 1212 48 0.9414834250662736 1212 49 -0.0669644054560834 1212 50 1.0862086100657606 1212 51 -1.2576332751866062 1213 1 -0.009522035009410956 1213 2 1.093453017651207 1213 3 0.8141305208594147 1213 4 0.6151003376138746 1213 5 0.2121707605931462 1213 6 0.12083057853362789 1213 7 -0.5947064338632586 1213 8 -2.2597873289394457 1213 9 -0.9252909058551921 1213 10 -0.24851837075194538 1213 11 0.037764441843641805 1213 12 -0.37523293760171583 1213 13 -0.8638369342377104 1213 14 -0.6553786428865038 1213 15 0.4743379657811033 1213 16 -0.8701265059491234 1213 17 1.124308897586765 1213 18 -0.35836956885295734 1213 19 -1.9744965992335608 1213 20 -1.3045759781664543 1213 21 0.4828208879667457 1213 22 -0.6034916069697402 1213 23 0.45853124223021524 1213 24 -0.45197979943226585 1213 25 -0.2272420884740569 1213 26 -1.4403701087059133 1213 27 -2.3178253130840005 1213 28 -0.7202709179821909 1213 29 0.147897761826016 1213 30 -0.8035544521509976 1213 31 -3.8068110897342464 1213 32 -2.6209713979771223 1213 33 -2.2624141678164023 1213 34 0.9721958147255805 1213 35 1.1775950212525146 1213 36 0.49203411639315675 1213 37 -1.065885144025863 1213 38 0.5597190198674156 1213 39 -0.772747210542299 1213 40 -0.17324012128276678 1213 41 -0.5348995357309789 1213 42 -0.3214574690042724 1213 43 0.6826966378618451 1213 44 0.0051994440484932625 1213 45 0.808947562719581 1213 46 0.5898392676697881 1213 47 0.7979063276603569 1213 48 1.1428723139551593 1213 49 -2.4211310721227477 1213 50 0.7112086100657606 1213 51 -0.952077719631049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 3.0 23 7.0 24 25.0 25 32.0 26 46.0 27 82.0 28 128.0 29 138.0 30 180.0 31 205.0 32 258.0 33 335.0 34 383.0 35 519.0 36 648.0 37 908.0 38 1663.0 39 4732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.339648304673076 13.008840959875645 9.657048479549209 48.99446225590207 2 13.783389995143274 16.745993200582806 51.51044196211754 17.960174842156388 3 14.23992229237494 15.191840699368624 45.79893152015542 24.769305488101022 4 11.403593977659057 37.027683341427874 29.89800874210782 21.670713938805246 5 10.509956289460904 25.97377367654201 50.02428363283147 13.491986401165615 6 21.622146673142304 55.41525012141817 13.453132588635262 9.509470616804274 7 18.99951432734337 21.826129188926664 22.408936376881982 36.765420106847984 8 15.794074793589122 53.132588635259836 13.715395823215154 17.357940747935892 9 15.784361340456531 14.327343370568238 15.094706168042741 54.793589120932495 10 12.452646915978631 46.46915978630403 20.621661000485673 20.456532297231668 11 23.768819815444388 18.416707139388052 38.21272462360369 19.601748421563865 12 15.055852355512384 20.93249150072851 21.748421563865953 42.26323457989315 13 18.892666342884894 14.861583292860612 46.935405536668284 19.310344827586206 14 16.056338028169016 15.269548324429335 18.523555123846528 50.15055852355512 15 17.455075279261777 19.378338999514327 20.310830500242837 42.85575522098106 16 39.72802331228752 19.543467702768332 15.065565808644973 25.662943176299173 17 17.63963088878096 43.44827586206896 17.027683341427878 21.884409907722198 18 45.24965999611424 22.04196619389936 16.97105109772683 15.73732271225957 19 19.057795046138903 23.603691112190383 16.522583778533267 40.81593006313744 20 22.26323457989315 18.552695483244293 15.774647887323944 43.40942204953861 21 14.23992229237494 17.552209810587662 17.532782904322485 50.675084992714915 22 16.318601262748906 39.45604662457504 17.60077707625061 26.62457503642545 23 15.075279261777563 21.185041282175813 16.726566294317628 47.013113161728995 24 39.2326372025255 23.156872268091306 17.532782904322485 20.077707625060707 25 21.57357940747936 40.86449732880039 16.338028169014084 21.22389509470617 26 46.50801359883439 19.757163671685284 17.13453132588635 16.600291403593978 27 19.28120446818844 45.886352598348715 19.397765905779504 15.43467702768334 28 22.758620689655174 17.94074793589121 19.834871296745995 39.46576007770762 29 16.629431762991747 23.234579893152016 40.14570179698883 19.990286546867413 30 15.658086449732881 17.591063623118018 22.185526954832444 44.56532297231666 31 14.910150558523554 40.65080135988344 24.98300145701797 19.456046624575034 32 17.10539096648859 42.52549781447305 19.69888295288975 20.670228266148616 33 15.444390480815931 24.97328800388538 18.591549295774648 40.99077221952404 34 17.756192326372027 21.942690626517727 39.92229237493929 20.378824672170957 35 38.572122389509474 16.94997571636717 22.156386595434675 22.321515298688684 36 19.524040796503154 16.192326372025253 48.936376881981545 15.347255949490043 37 21.175327829043226 39.45604662457504 21.583292860611948 17.78533268576979 38 14.706168042739193 17.8630403108305 49.62603205439534 17.80475959203497 39 16.270033997085964 21.019912578921808 22.816901408450704 39.893152015541524 40 13.754249635745508 16.474016512870328 50.02428363283147 19.747450218552697 41 14.997571636716852 15.386109762020398 22.253521126760564 47.36279747450218 42 37.0082564351627 16.658572122389508 27.042253521126757 19.29091792132103 43 16.6682855755221 13.841670713938806 44.157357940747936 25.332685769791162 44 19.13550267119961 39.68916949975716 23.83681398737251 17.338513841670714 45 11.38416707139388 17.29966002914036 51.22875182127247 20.087421078193298 46 36.7557066537154 22.156386595434675 22.146673142302088 18.94123360854784 47 13.919378338999513 15.541525012141816 27.11996114618747 43.4191355026712 48 15.638659543467703 18.203011170471104 20.06799417192812 46.090335114133076 49 36.29917435648373 17.833899951432734 19.58232151529869 26.28460417678485 50 15.2986886838271 16.920835356969402 43.3317144244779 24.448761534725595 51 15.220981058766391 17.833899951432734 22.952889752306945 43.99222923749393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 1.0 17 0.5 18 0.0 19 1.0 20 2.0 21 1.0 22 0.0 23 0.5 24 1.0 25 1.0 26 1.0 27 1.0 28 7.0 29 13.0 30 19.5 31 26.0 32 40.0 33 54.0 34 95.5 35 137.0 36 164.0 37 191.0 38 274.5 39 358.0 40 419.5 41 481.0 42 464.0 43 447.0 44 567.5 45 688.0 46 771.0 47 854.0 48 1041.0 49 1228.0 50 2479.0 51 3730.0 52 2267.0 53 804.0 54 685.5 55 567.0 56 459.5 57 352.0 58 280.5 59 209.0 60 139.5 61 70.0 62 48.5 63 27.0 64 20.5 65 14.0 66 13.0 67 12.0 68 9.0 69 6.0 70 6.5 71 7.0 72 5.0 73 3.0 74 4.0 75 2.5 76 0.0 77 2.0 78 4.0 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01942690626517727 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.009713453132588635 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 10295.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.47984458474988 #Duplication Level Percentage of deduplicated Percentage of total 1 65.68894952251023 18.708110733365714 2 9.27694406548431 5.284118504128218 3 6.446111869031378 5.507527926177756 4 5.081855388813096 5.789218067022826 5 3.069577080491132 4.3710539096648855 6 2.251023192360164 3.8465274405050995 7 1.8076398362892223 3.603691112190384 8 1.2619372442019101 2.8751821272462363 9 0.9890859481582538 2.535211267605634 >10 4.024556616643929 16.41573579407479 >50 0.0 0.0 >100 0.034106412005457026 2.768334142787761 >500 0.034106412005457026 5.8669256920835355 >1k 0.034106412005457026 22.42836328314716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC 2309 22.42836328314716 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG 604 5.8669256920835355 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT 285 2.768334142787761 No Hit GTTATGAAGCCTAGAACGATAAAGAGGCATATCGTACAAGGATGCAAAAAC 41 0.39825157843613407 No Hit AAATCATGAGCATCAAAACGCTCACCTTTCAGCGCAAGAAATACATCACCA 35 0.3399708596406022 No Hit GCAGATGAAAATTCAAATTGCTGCGATTTAACTGTTTCTAGTCGCTGTTTT 32 0.3108305002428363 No Hit CACTTACGTGAAGGGACTGAATGTGCTGATCAACGTGTACTTGAAGCCGTT 31 0.30111704711024767 No Hit TAAACGCCATTCAGGAAAATACATCATGAAATCCCTGTCCTTACTCATCAC 27 0.26226323457989315 No Hit GGGCTATTTTTGGCACACTGGGCTTTGGCTTTTGGTTCTTTTTCTACACCT 26 0.2525497814473045 No Hit GCAGTAATCAACCAACCCCATGCAAACATTGGCATTTTCATCAATGTCATG 25 0.24283632831471588 No Hit AGACAAGACTGGATCTGATCAAGCGCTTCTATGATGCGGTGTCTACTTTCA 24 0.23312287518212726 No Hit TACACATACCGCAGATGGCTTGAAAGTGGCGCTTGAACACCGTAATCCACA 24 0.23312287518212726 No Hit TATCACGACTCGTTCGACCCCATTCGCGCAGTGCGAACAAAAGACTTCAGT 23 0.22340942204953862 No Hit ATGCGAGACCGTTTCGGAGCCTTAACCTAATGTTGAACCGGGGTAATTAGG 22 0.21369596891694995 No Hit GTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTT 22 0.21369596891694995 No Hit GTAGGCACACGCTTGTTCGGTAAAGTATCTAACTTGACTGACTACGGTGCT 22 0.21369596891694995 No Hit CTTCAGTAAATCGGGCACGCACCGACCAGCCACAATCAGAAACCTGTCTCT 21 0.20398251578436136 No Hit AAAAAGCATTGTGCTTGAGTTACAACAACTGATCTGGCCTAACTATGCAGA 21 0.20398251578436136 No Hit CTATAAAGACACAAGCACACGTATGTTTATTGCAGCACTATTCACAATAGC 21 0.20398251578436136 No Hit GACCTTAACGGGGGCTAAAAACATTCAAGCGATTAACTCAGATTGCTTGGT 20 0.19426906265177268 No Hit GGCCAGTACCACCGCAAGTTCATGCTGCAGCTTATATCGAGCCAGCTTGAT 20 0.19426906265177268 No Hit CGCCAGATCAGTTTTTCCACATACAGCTTTTCCGTGATGTCGCGCGCAATT 20 0.19426906265177268 No Hit CTTCCATCTCCCCAGCCAACTCTTACGTATCTTTTTAGGGTCATCTTAAGC 19 0.18455560951918407 No Hit GCGCAGGACCCCTTATTTAGACCAGTGAGCTGTTACGCTTTCTTTAAAGGA 18 0.17484215638659545 No Hit GGCTAAGCCGGTAGCCGATCCTTAGTCCCTCTTCTAGATTTCTTGAAGAGG 18 0.17484215638659545 No Hit CATACAGCCAATCCGTGCTCTCATCTTGCATTGGAATAGATAGCGTTAGGC 18 0.17484215638659545 No Hit GTGGTGAATTCGCCATGAAAAGTATTGCTTATCGCGCAAAGGGTTTTATGT 16 0.15541525012141816 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 16 0.15541525012141816 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 16 0.15541525012141816 No Hit GAGCGCGTAGCTGAAACATGCCGATTGATGATTGCAAACGGATTCCTCGTT 16 0.15541525012141816 No Hit AGTAGGATATAGTGATCCGATGATTCAGAATGGAATGATCATCGCTCAAGA 16 0.15541525012141816 No Hit CGCCTGTAATCCCAGCTACTCAGGAGGCTGAGACAGGAGAATAACTTGAAC 16 0.15541525012141816 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 16 0.15541525012141816 No Hit GACTGGGTTTGAGGTGAAAGATGTGTGTGTATTTGGCAGCAGTTAAAGGGC 15 0.1457017969888295 No Hit ATCAATCAGTGCGGAAATCTCTGGTGGAAGTTTTTCGACAGAATCAAAACC 15 0.1457017969888295 No Hit TGTTTGGCCTGATAAATGCGGATAGCCTCTTGCGTTTTAAGGCCAGGAAAA 15 0.1457017969888295 No Hit GCTATGTTGGGCAAACCCCTTCCTCCTAAGTTGTTCTGTGGCACTCTCAAA 15 0.1457017969888295 No Hit CCGCTGTACCGCCGGATTATTTTGAGAGTTTTAACCGAAAACGTGAGAACT 14 0.1359883438562409 No Hit GGTCTATGCAGTGCAATCGATTATCAGTGGCCCGCTTGATCGCGCCAAACT 14 0.1359883438562409 No Hit CACCTGGCAAAATGACGGCACAATAGGCTCACAGCCTGTCTCTTATACACA 14 0.1359883438562409 No Hit GGGCTATTGTGTATCTTAACCAGCATCTCAATCAAGCTATCTCAAACCGTA 14 0.1359883438562409 No Hit CGCCTGCAGAAAGGACTAAACCATCGATTTTTTTCCCTTCCAAATGGCCCA 14 0.1359883438562409 No Hit TGCCAATCCCGATGTGATCGTTACCGATATCATGATGCCCTATGCCTCCGG 14 0.1359883438562409 No Hit CTTCTGTCCAACCCGGAATAACCTGGTTAAGTCCAAATGAAATAGGTTGAC 14 0.1359883438562409 No Hit CCTGTTAGTTCTAAGGCAGTCTCTAAATTTCCCAAGAGGTAAAGCAGAAAG 13 0.12627489072365225 No Hit GCCACAGGACGTAAGCAAGCATCAAGCATTTGCATTCCACTACAATTAACC 13 0.12627489072365225 No Hit GTGAAATAGAACCTGAAACCGTACGCCTACAAGCGGTCGGAGCACCAATAA 13 0.12627489072365225 No Hit GATAAGGATTGGCTGATAGAAGAATGCAAGAAAAGATTTATCGTCAGTGGC 13 0.12627489072365225 No Hit GGTTTAATGGCAAGATCTTTCTTTTCACCGTAAACGAAGTTAGGTCCCATA 13 0.12627489072365225 No Hit CTCAGAGACCACGGCTGGAGTAATAAGCTTATAGTTCGGAACCTCCTTACA 13 0.12627489072365225 No Hit GGTTAAAAACTGCGGGGATGAGCTGTGGGTAGGGGTGAAAGGCCAATCAAA 13 0.12627489072365225 No Hit GAGCTGATGACGAGTTGCCTTTAGGCGACGAAGTGGTTGATGCCATGCTTC 13 0.12627489072365225 No Hit AGGCTACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATATAG 13 0.12627489072365225 No Hit CTCGTAAGTAGCTATTGAACAAAAGAATGGTAGATAAGGGTAGCGATTCAT 13 0.12627489072365225 No Hit CACCCAGACATTGCTGATCACGTTCATGATGATGACCAGCAGGCCCATGGC 13 0.12627489072365225 No Hit CAGCAGCAAGAATTAGCCCGTTTTGGTTAATTCTTGCTGATACCAGCCGAT 13 0.12627489072365225 No Hit CCTGTACCCAAGCCAAGATAAATCAATCCATTAGCTGGCTTGCCTGCTAAA 13 0.12627489072365225 No Hit GCTAAGCATGTGAGCTTGTCTTGGGAGTGTTTTTGATGCTTCTGGGGTGGG 13 0.12627489072365225 No Hit ATACAGCAGCGTGATATGGATGTCGCTCAATTGGCGGCGATTTTGCAAACA 13 0.12627489072365225 No Hit GCTGCCAACGGCGCAGTGTCACCGGTACGTAAATTTGTACCAGACGAAATT 12 0.11656143759106363 No Hit CCCACATCCTTTTCCACTTAGCACACCCTTAGGGGCCTGTCTCTTATACAC 12 0.11656143759106363 No Hit GCTTCAGCCTCATTGAAAAGTGCAAGAGGAAACAATCAGAAGCAGGTTATA 12 0.11656143759106363 No Hit TTCATGGTTTGTATTCACACTGAAAATCCCGTACTCTGCGTTGATACCTGT 12 0.11656143759106363 No Hit GATGTGTATAAGAGACAGACGCGTACCAGTTTGCCCTGTGGTTGCTGTAGT 12 0.11656143759106363 No Hit CCTTTTGCTTTTTCTCCATGTCACGGTACTGGCCCTGGTACTGTCTCTTAT 12 0.11656143759106363 No Hit ATGCATGAGTTACTTGATGAAGAGGTAGATCTGATGATCAAGGCCTGTCTC 12 0.11656143759106363 No Hit CTCCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAA 12 0.11656143759106363 No Hit CCCTCGTGCAATCAGTAAATAATAGGGACTCTTACGTTTACATAAACCCAC 12 0.11656143759106363 No Hit CAGACATATTCGGTGTACCGTTGGACTCACCAGTTGATGTACTGTCTCTTA 12 0.11656143759106363 No Hit ACATGTTGTGGTTGACTCCTATTATTCCTTGTCCAAACCACAATACGTCAT 12 0.11656143759106363 No Hit AAGGAGCCGATGAGGTACCTACAGTTGTCGGGTCGAAAGACAACGAAGCAG 12 0.11656143759106363 No Hit CAAAATCCCACTGCTCAAGTTCTTCATCAAGCACGAGCTGATCTACGTGCC 12 0.11656143759106363 No Hit GTATAAAGTTCACTCGGGCGGGAAGTTTCCCGCATCAGCTTCATCCATGCA 12 0.11656143759106363 No Hit GGAATCTGAGTATTGTAGCAACCAGCTTCAATCATGCCGTGATAACAATGC 12 0.11656143759106363 No Hit AGCGGGTGCCGCACCATTCAATGTTAGATTTGAGATTAAGCTTGATGGGAA 12 0.11656143759106363 No Hit CCGGAAACAAGGCACGAGAAGCAGCCCAAGTGATATTTTGCACTGGTAACA 12 0.11656143759106363 No Hit CACTACATCCGGGCAGTTATGGCAGCTGAGCGAGATGTAGGTCTCAAAATG 12 0.11656143759106363 No Hit GACTCGACTAGTGAGCTATTACGCTTTCTTTAAAGGGTGGCTGCTTCTAAG 11 0.10684798445847497 No Hit GATCAAATCATCAAACTTGGCAATACCCATACTTGCCATTAACTCACGCAC 11 0.10684798445847497 No Hit ATCCAGATCAAATACCTGCACGGTCAGGAATACTCGGTGGACCAGGCCTGT 11 0.10684798445847497 No Hit GCGAGATGTGGTCAACCCAAAGTCCAGGGAATGTGGTTGCATGTCGTAATA 11 0.10684798445847497 No Hit CACTGAGTAGAAGCTCCGAAAGATGATGCCGTGGAGGGCTGTCTCTTATAC 11 0.10684798445847497 No Hit TTCCTGTACCACCTAGTATCGTTTTAATCGATGGGGGGACGCAGTAGGATA 11 0.10684798445847497 No Hit ATAAAGGTACTGATCAAGGTTTTGAACGAGTTTCTGGCCTCAACCAGGCTA 11 0.10684798445847497 No Hit GTTGGAACTGGCTTGCAAATATTTCACCATAAAGCGTGTCAGTGCGCCAAA 11 0.10684798445847497 No Hit AGCCCCTTTGTTTTGGCCAATTTCTCCCATTTACAATGGGTGTAGTAGCCA 11 0.10684798445847497 No Hit GATGTCTTTTCCTCGAGTTACTTAGATGTTTCAGTTCACTCGGTTCGCCTC 11 0.10684798445847497 No Hit CTTCTATCCCATCAATCCTCAGAAGATTGGCTTCACCCCCTTGTGGAAAAA 11 0.10684798445847497 No Hit AAACAATTTACCGTGAGTCTGTTTTCGTTAATTCGCTGACAGATCAAGACC 11 0.10684798445847497 No Hit CCTCAAAGATGGACAGTTTGAGGCAAGGATGACAAGTTTTGCTTTGCCTTG 11 0.10684798445847497 No Hit CATACCTATTGTCCCAGCTACTCAAGAGGCTGAAGCAGGAGGATCTGTTGC 11 0.10684798445847497 No Hit GAATGAACGACTCGAAGAGCATCGTCAAAATTTAGCATTTGCGCATGAAAC 11 0.10684798445847497 No Hit GGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAA 11 0.10684798445847497 No Hit GCTATAATAGAGCGAGATATCCACGGAGACGTGTTGAAAGCGGGGTATAGT 11 0.10684798445847497 No Hit CTACTTTGGTGCGTTTACCTTCAAATGCGAATGGCTTGGTCACCACTGCGA 11 0.10684798445847497 No Hit ATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATCCCGTACTCTGCGTTGA 11 0.10684798445847497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.10684798445847499 2 0.0 0.0 0.17484215638659542 3 0.0 0.0 0.21369596891694997 4 0.0 0.0 0.2719766877124818 5 0.0 0.0 0.4662457503642545 6 0.0 0.0 0.4759592034968431 7 0.0 0.0 0.505099562894609 8 0.0 0.0 0.5148130160271976 9 0.0 0.0 0.6896551724137931 10 0.0 0.0 0.9130645944633318 11 0.0 0.0 0.9130645944633318 12 0.0 0.0 0.9713453132588635 13 0.0 0.0 1.010199125789218 14 0.0 0.0 1.0587663914521612 15 0.0 0.0 1.0781932977173385 16 0.0 0.0 1.1073336571151045 17 0.0 0.0 1.1364740165128704 18 0.0 0.0 1.146187469645459 19 0.0 0.0 1.175327829043225 20 0.0 0.0 1.3793103448275863 21 0.0 0.0 1.5638659543467703 22 0.0 0.0 1.8067022826614862 23 0.0 0.0 1.9621175327829043 24 0.0 0.0 2.20495386109762 25 0.0 0.0 2.282661486158329 26 0.0 0.0 2.4672170956775132 27 0.0 0.0 3.011170471102477 28 0.0 0.0 3.3608547838756677 29 0.0 0.0 3.5551238465274406 30 0.0 0.0 3.836813987372511 31 0.0 0.0 4.40990772219524 32 0.0 0.0 5.157843613404565 33 0.0 0.0 6.197183098591549 34 0.0 0.0 7.323943661971831 35 0.0 0.0 8.586692569208354 36 0.0 0.0 9.655172413793103 37 0.0 0.0 11.471588149587179 38 0.0 0.0 12.617775619232637 39 0.0 0.0 14.171928120446818 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 265 0.0 45.0 45 ATGCCGT 310 0.0 38.467743 44 GTATGCC 380 0.0 31.381578 42 TATGCCG 380 0.0 31.381578 43 CGTATGC 385 0.0 30.974024 41 ATCTCGT 380 0.0 30.789473 37 TCTCGTA 380 0.0 30.789473 38 TCGTATG 380 0.0 30.789473 40 CTCGTAT 380 0.0 30.789473 39 GTATCTC 370 0.0 30.405403 35 TATCTCG 380 0.0 30.197369 36 CTGTATC 380 0.0 30.197369 33 TGTATCT 375 0.0 30.0 34 CGAGACT 400 0.0 29.8125 23 ACTGTAT 385 0.0 29.805193 32 CAACTGT 385 0.0 29.805193 30 TCAACTG 385 0.0 29.805193 29 ACTCAAC 385 0.0 29.805193 27 GACTCAA 385 0.0 29.805193 26 CTCAACT 385 0.0 29.805193 28 >>END_MODULE