FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_67.34100000004c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_67.34100000004c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615082
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAT300.00514464730.00092111
GGTATCA4600.026.9161281
GTATCAA9700.024.3683031
CTCGTAT802.9723305E-622.49886339
ACGCGGG500.00226408722.4988638
TTACACG702.7014097E-522.4988614
GTAAGTA1156.7575456E-921.5206492
ACACGCG754.5999586E-520.998946
TTTGCGT754.5999586E-520.998943
CGGTGCC550.003933788720.45517310
AACCCGT550.003935641620.4535126
TATGCCG908.244613E-619.9989943
TAGAGTG706.980794E-419.2847395
TTTTGCG951.314781E-518.946412
CTATAAT600.00650903518.7490524
CACGCGG600.00650903518.7490527
CATCTCC21350.018.54707145
ACCTCGG851.2030025E-418.5299815
TATCAAC13050.018.1025332
GTCCTAT750.001105570718.0093381
Produced by FastQC (version 0.11.2)