FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_64.34100000004c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_64.34100000004c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297347
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC176985.9519685754354335No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC15710.5283389440619882No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCT11200.37666430130453643No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG11170.37565537906889934No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC10800.36321200482937444No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC9860.33159910811274373No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACA9470.31848311904946075No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGT8610.2895606816278624No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT8490.2855249926853138No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAA7170.2411324143172791No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG6730.22633488819460093No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGA6640.22330812148768944No Hit
TTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTCT6610.22229919925205233No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAG6540.21994504736889894No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTC6000.20178444712743027No Hit
CCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAG5060.1701715504107995No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGA4960.16680847629200898No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATA4940.1661358614682509No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTAT4940.1661358614682509No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAA4580.15402879464060507No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT4390.14763895381490313No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACC4280.14393957228423357No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTG4280.14393957228423357No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG4140.13923126851792686No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG3910.13149619804470872No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCA3860.12981466098531347No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG3810.1281331239259182No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACT3720.12510635721900673No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTG3700.12443374239524865No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG3640.12241589792397435No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC3550.11938913121706289No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAAC3470.11669867192203048No Hit
GAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAG3290.11064513850820759No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA3280.11030883109632854No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAG3150.10593683474190087No Hit
GTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAG3130.10526421991814278No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG3120.10492791250626372No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG3110.10459160509438468No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGA3090.10391899027062657No Hit
GAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATT3040.10223745321123132No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGA3000.10089222356371513No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCA3000.10089222356371513No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTG253.8840633E-544.999518
GGATAAT207.0252287E-444.9994966
TCGATCG207.0252287E-444.99949616
TGCCGTC16650.041.62815545
TATGCCG16850.041.26066643
GTATGCC16850.041.26066642
ATGCCGT16800.041.2495444
CTCGTAT16800.041.11561239
TCGTATG16950.040.884540
TCTCGTA17000.040.63189738
ATCTCGT17150.040.4077137
AATCTCG16750.039.89507736
ATTAAAT17000.039.70543732
CGTATGC17650.039.3904941
CTGATTA17200.039.3745629
TGATTAA17100.039.21008330
CGAGACC17550.038.97392323
ACGAGAC18050.038.8915322
ACCTGAT17450.038.8104527
GACCTGA17450.038.8104526
Produced by FastQC (version 0.11.2)