FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_63.34100000004c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_63.34100000004c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565448
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC38400.6791075395084959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAAT4100.042.25510432
CTGATTA4400.039.8854229
TATGCCG4750.039.78853243
CTCGTAT4900.039.48886539
TCGTATG4850.038.96815540
TTAAATC4550.038.07602333
TGATTAA4750.037.4201730
ATGCCGT5100.037.05794544
TAAATCT4700.036.86083234
TCTCGTA5250.036.85627438
CGTATGC5150.036.69816641
ACGAGAC5200.036.345322
GATTAAA4850.035.7208131
TGCCGTC5400.035.4189745
GCCCACG5450.035.09401718
CCCACGA5400.034.99917619
GTATGCC5500.034.36282742
CACGAGA5550.034.0532521
CGAGACC5250.033.85634623
CGAGCCC5650.033.85175315
Produced by FastQC (version 0.11.2)