FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_61.34100000004bed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_61.34100000004bed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622086
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA207.033015E-444.99710531
TATGCCG1050.032.14079343
TATCACG501.9806812E-631.4979722
GTATTAA300.005130917830.0173721
TCGTATG1150.029.3459440
CGTATGC1300.025.9598741
CTCGTAT1300.025.9598739
CGCCGGT450.001226381924.9983927
TCTCGTA1450.023.27436438
CTCGACG500.002262182822.5021713
GCTATAT500.002264297322.49855232
AACGACG654.2324627E-420.77123315
GCTCCGT654.2324627E-420.77123326
ACGGAGC654.2370582E-420.7678958
CACGAGA1750.020.57010721
CTACTAA550.003921365420.466391
TCGACGC550.0039323420.4565214
TGTGTCG550.0039323420.4565226
CGCAATG550.003934171520.45487418
ACCGTCG550.00393600420.4532328
Produced by FastQC (version 0.11.2)