##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_60.34100000004bd0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2667 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.318335208098986 38.0 32.0 38.0 27.0 38.0 2 34.51368578927634 38.0 32.0 38.0 27.0 38.0 3 34.68466441694788 38.0 32.0 38.0 32.0 38.0 4 34.94638170228721 38.0 32.0 38.0 32.0 38.0 5 35.04724409448819 38.0 32.0 38.0 32.0 38.0 6 36.52718410198725 38.0 38.0 40.0 32.0 40.0 7 36.16310461192351 38.0 38.0 40.0 32.0 40.0 8 36.12410948631421 38.0 38.0 40.0 32.0 40.0 9 36.181102362204726 38.0 38.0 40.0 32.0 40.0 10 36.05586801649794 38.0 38.0 40.0 32.0 40.0 11 36.01949756280465 38.0 38.0 40.0 27.0 40.0 12 35.9238845144357 38.0 38.0 40.0 32.0 40.0 13 36.14660667416573 38.0 38.0 40.0 32.0 40.0 14 35.83277090363705 38.0 38.0 40.0 27.0 40.0 15 35.8350206224222 38.0 38.0 40.0 27.0 40.0 16 35.59992500937383 38.0 32.0 40.0 27.0 40.0 17 35.62692163479565 38.0 38.0 40.0 27.0 40.0 18 35.64679415073116 38.0 32.0 40.0 27.0 40.0 19 35.93963254593176 38.0 38.0 40.0 27.0 40.0 20 35.63817022872141 38.0 38.0 40.0 27.0 40.0 21 35.79152605924259 38.0 38.0 40.0 27.0 40.0 22 35.8721409823772 38.0 38.0 40.0 27.0 40.0 23 35.9392575928009 38.0 38.0 40.0 27.0 40.0 24 35.74015748031496 38.0 38.0 40.0 27.0 40.0 25 35.58417697787777 38.0 38.0 40.0 27.0 40.0 26 35.608173978252715 38.0 38.0 40.0 27.0 40.0 27 35.33408323959505 38.0 32.0 40.0 27.0 40.0 28 35.399325084364456 38.0 32.0 40.0 27.0 40.0 29 35.25909261342332 38.0 32.0 40.0 27.0 40.0 30 35.435320584926885 38.0 32.0 40.0 27.0 40.0 31 35.28383952005999 38.0 32.0 40.0 27.0 40.0 32 35.28121484814398 38.0 32.0 40.0 27.0 40.0 33 35.382452193475814 38.0 32.0 40.0 27.0 40.0 34 35.35058117735283 38.0 38.0 40.0 27.0 40.0 35 35.22272215973003 38.0 32.0 40.0 27.0 40.0 36 35.1822272215973 38.0 32.0 40.0 27.0 40.0 37 35.4750656167979 38.0 32.0 40.0 27.0 40.0 38 35.49943757030371 38.0 32.0 40.0 27.0 40.0 39 35.4079490063742 38.0 38.0 40.0 27.0 40.0 40 35.44881889763779 38.0 32.0 40.0 27.0 40.0 41 35.37120359955006 38.0 38.0 40.0 27.0 40.0 42 35.341207349081365 38.0 32.0 40.0 27.0 40.0 43 35.58942632170979 38.0 38.0 40.0 27.0 40.0 44 35.40869891263592 38.0 38.0 40.0 27.0 40.0 45 35.429696287964006 38.0 32.0 40.0 27.0 40.0 46 35.184101987251594 38.0 32.0 40.0 27.0 40.0 47 35.3472065991751 38.0 32.0 40.0 27.0 40.0 48 35.2819647544057 38.0 32.0 40.0 27.0 40.0 49 35.203599550056246 38.0 32.0 40.0 27.0 40.0 50 35.28871391076115 38.0 32.0 40.0 27.0 40.0 51 34.448068991376076 38.0 32.0 38.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -3.346316964285702 2201 2 -0.5376860119047535 2201 3 -0.8841517857142733 2201 4 2.893303571428575 2201 5 -5.69352678571428 2201 6 -1.3065104166666686 2201 7 -1.3041294642857153 2201 8 -3.257068452380963 2201 9 -1.2260416666666742 2201 10 -6.2539806547619 2201 11 -5.748921130952372 2201 12 -4.855133928571437 2201 13 3.525148809523813 2201 14 -1.9655505952380992 2201 15 -5.543303571428563 2201 16 -3.1817336309523796 2201 17 -5.059486607142862 2201 18 0.6686755952380921 2201 19 0.3871651785714221 2201 20 -1.0995535714285722 2201 21 0.19155505952380025 2201 22 -0.236049107142847 2201 23 2.437276785714282 2201 24 0.9390997023809504 2201 25 1.4193452380952465 2201 26 3.5183779761904717 2201 27 -8.598028273809526 2201 28 2.8309523809523824 2201 29 0.4733258928571473 2201 30 3.1880580357142847 2201 31 -5.2209449404761905 2201 32 -4.24683779761904 2201 33 -0.14367559523810058 2201 34 -6.628683035714285 2201 35 -6.912611607142868 2201 36 0.22905505952381588 2201 37 -0.06960565476191505 2201 38 -0.8381324404762012 2201 39 -2.970684523809517 2201 40 -7.2712053571428505 2201 41 -7.2765997023809526 2201 42 -2.628608630952378 2201 43 1.0290922619047649 2201 44 -8.606882440476188 2201 45 -2.1909598214285637 2201 46 -3.9148065476190546 2201 47 -4.718526785714285 2201 48 1.0474330357142776 2201 49 1.5931175595238045 2201 50 1.9441592261904788 2201 51 -4.640997023809522 1101 1 -1.0129836309523697 1101 2 2.2623139880952436 1101 3 0.7158482142857281 1101 4 1.6933035714285722 1101 5 1.4398065476190496 1101 6 1.8268229166666643 1101 7 0.8958705357142875 1101 8 2.1429315476190354 1101 9 2.1739583333333243 1101 10 -1.7206473214285651 1101 11 3.784412202380963 1101 12 4.011532738095234 1101 13 3.39181547619048 1101 14 3.834449404761898 1101 15 -0.009970238095228012 1101 16 -1.9817336309523768 1101 17 -0.7928199404761926 1101 18 0.8686755952380949 1101 19 -2.612834821428578 1101 20 -1.299553571428575 1101 21 1.391555059523803 1101 22 2.56395089285715 1101 23 1.6372767857142847 1101 24 3.0724330357142833 1101 25 -1.4473214285714207 1101 26 1.8517113095238074 1101 27 3.001971726190476 1101 28 2.8309523809523824 1101 29 -0.9266741071428513 1101 30 -1.2786086309523768 1101 31 1.845721726190476 1101 32 0.019828869047628928 1101 33 -0.9436755952380977 1101 34 -1.9620163690476176 1101 35 -1.8459449404762012 1101 36 4.029055059523813 1101 37 1.5303943452380864 1101 38 1.1618675595237988 1101 39 3.4959821428571445 1101 40 2.2621279761904844 1101 41 0.8567336309523768 1101 42 -1.5619419642857153 1101 43 0.5624255952381034 1101 44 2.326450892857146 1101 45 2.8090401785714363 1101 46 3.951860119047616 1101 47 2.548139880952384 1101 48 -1.8192336309523895 1101 49 -0.8735491071428569 1101 50 2.2108258928571445 1101 51 3.025669642857146 2202 1 -0.26298363095236965 2202 2 -8.537686011904754 2202 3 -4.134151785714273 2202 4 -2.856696428571425 2202 5 1.1398065476190524 2202 6 -0.47317708333333286 2202 7 -4.554129464285715 2202 8 -6.007068452380963 2202 9 0.27395833333332575 2202 10 2.079352678571432 2202 11 -6.66558779761904 2202 12 3.311532738095231 2202 13 -3.5581845238095227 2202 14 -6.215550595238099 2202 15 1.7900297619047691 2202 16 -4.43173363095238 2202 17 -11.392819940476194 2202 18 -1.581324404761908 2202 19 -2.112834821428578 2202 20 -1.3495535714285722 2202 21 -5.5584449404762 2202 22 -4.486049107142847 2202 23 -0.31272321428571814 2202 24 -5.477566964285714 2202 25 -2.247321428571418 2202 26 1.3517113095238074 2202 27 -2.0980282738095255 2202 28 1.3309523809523824 2202 29 0.9733258928571473 2202 30 -0.4786086309523796 2202 31 2.4457217261904773 2202 32 3.9198288690476275 2202 33 -2.8936755952381006 2202 34 -4.212016369047618 2202 35 -4.9959449404762 2202 36 -1.0209449404761841 2202 37 3.430394345238085 2202 38 0.6618675595237988 2202 39 1.6959821428571473 2202 40 -4.187872023809518 2202 41 0.8067336309523796 2202 42 -0.7119419642857139 2202 43 2.3624255952381006 2202 44 2.7264508928571445 2202 45 -1.4409598214285637 2202 46 -4.998139880952387 2202 47 -2.0518601190476176 2202 48 -0.1192336309523867 2202 49 1.4264508928571402 2202 50 -3.139174107142857 2202 51 0.5256696428571459 1102 1 2.9870163690476303 1102 2 3.4623139880952465 1102 3 0.11584821428572667 1102 4 2.893303571428575 1102 5 0.6398065476190524 1102 6 2.360156250000003 1102 7 2.6958705357142847 1102 8 3.0762648809523725 1102 9 2.44062499999999 1102 10 2.4126860119047677 1102 11 3.2510788690476247 1102 12 3.478199404761895 1102 13 3.525148809523813 1102 14 4.034449404761901 1102 15 3.123363095238105 1102 16 2.151599702380956 1102 17 2.607180059523806 1102 18 2.3353422619047564 1102 19 3.387165178571422 1102 20 3.9004464285714278 1102 21 1.8582217261904646 1102 22 2.0972842261904887 1102 23 3.437276785714282 1102 24 3.272433035714286 1102 25 2.086011904761918 1102 26 4.185044642857143 1102 27 3.4019717261904745 1102 28 3.164285714285718 1102 29 3.806659226190483 1102 30 2.854724702380956 1102 31 2.779055059523813 1102 32 3.086495535714292 1102 33 3.5229910714285637 1102 34 3.7046502976190467 1102 35 2.7540550595238003 1102 36 2.5623883928571516 1102 37 2.2637276785714207 1102 38 1.8285342261904631 1102 39 2.6959821428571473 1102 40 3.3954613095238173 1102 41 4.390066964285715 1102 42 4.038058035714286 1102 43 3.029092261904765 1102 44 4.05978422619048 1102 45 4.142373511904772 1102 46 -1.7481398809523867 1102 47 3.9481398809523824 1102 48 3.3807663690476133 1102 49 3.92645089285714 1102 50 1.7774925595238074 1102 51 3.025669642857146 2203 1 2.7870163690476275 2203 2 -2.737686011904753 2203 3 3.1158482142857267 2203 4 2.893303571428575 2203 5 0.23980654761905384 2203 6 0.6268229166666686 2203 7 -2.9041294642857167 2203 8 -2.457068452380966 2203 9 0.3739583333333272 2203 10 0.8793526785714292 2203 11 3.3844122023809575 2203 12 3.611532738095228 2203 13 0.3918154761904802 2203 14 3.4344494047618994 2203 15 0.990029761904772 2203 16 1.4182663690476218 2203 17 1.8071800595238088 2203 18 3.0686755952380906 2203 19 1.587165178571425 2203 20 1.700446428571425 2203 21 1.391555059523803 2203 22 2.1639508928571516 2203 23 1.237276785714279 2203 24 -5.1275669642857125 2203 25 -1.4473214285714207 2203 26 2.451711309523809 2203 27 0.8019717261904731 2203 28 2.0309523809523853 2203 29 1.6733258928571502 2203 30 -0.27860863095237676 2203 31 -1.7542782738095255 2203 32 4.219828869047625 2203 33 2.456324404761901 2203 34 -1.562016369047619 2203 35 1.3540550595238017 2203 36 3.229055059523816 2203 37 1.730394345238082 2203 38 3.3618675595238017 2203 39 3.4959821428571445 2203 40 3.46212797619048 2203 41 4.256733630952382 2203 42 -2.3619419642857125 2203 43 1.962425595238102 2203 44 2.326450892857146 2203 45 3.209040178571435 2203 46 -1.4481398809523895 2203 47 -6.251860119047617 2203 48 -3.0192336309523853 2203 49 -2.873549107142857 2203 50 3.0108258928571416 2203 51 1.025669642857146 1103 1 1.5870163690476318 1103 2 2.2623139880952436 1103 3 3.1158482142857267 1103 4 2.893303571428575 1103 5 0.23980654761905384 1103 6 2.6268229166666686 1103 7 2.8958705357142875 1103 8 2.1429315476190354 1103 9 2.573958333333323 1103 10 2.4793526785714306 1103 11 4.184412202380962 1103 12 2.4115327380952323 1103 13 2.591815476190476 1103 14 3.834449404761898 1103 15 2.5900297619047663 1103 16 1.0182663690476232 1103 17 3.807180059523809 1103 18 3.868675595238095 1103 19 3.587165178571425 1103 20 4.100446428571431 1103 21 2.9915550595237974 1103 22 2.1639508928571516 1103 23 3.6372767857142847 1103 24 3.0724330357142833 1103 25 3.5526785714285793 1103 26 4.05171130952381 1103 27 1.6019717261904773 1103 28 2.430952380952384 1103 29 2.0733258928571487 1103 30 1.7213913690476232 1103 31 1.2457217261904745 1103 32 3.019828869047629 1103 33 2.0563244047619023 1103 34 2.2379836309523853 1103 35 3.154055059523799 1103 36 3.6290550595238145 1103 37 4.130394345238088 1103 38 1.7618675595238003 1103 39 3.095982142857146 1103 40 3.46212797619048 1103 41 3.456733630952378 1103 42 3.8380580357142833 1103 43 3.1624255952380977 1103 44 1.9264508928571473 1103 45 2.009040178571439 1103 46 3.951860119047616 1103 47 3.7481398809523796 1103 48 2.780766369047612 1103 49 2.5264508928571416 1103 50 3.810825892857146 1103 51 -1.5743303571428555 2204 1 -3.0129836309523697 2204 2 -2.0376860119047535 2204 3 -9.384151785714273 2204 4 0.14330357142857508 2204 5 1.1398065476190524 2204 6 1.0268229166666671 2204 7 -3.5541294642857153 2204 8 0.4929315476190368 2204 9 -0.47604166666667425 2204 10 -2.920647321428568 2204 11 -2.6655877976190396 2204 12 -1.6884672619047691 2204 13 0.6918154761904773 2204 14 -4.215550595238099 2204 15 1.7900297619047691 2204 16 0.3182663690476204 2204 17 3.107180059523806 2204 18 3.168675595238092 2204 19 -4.862834821428578 2204 20 -2.8495535714285722 2204 21 1.1915550595238003 2204 22 1.763950892857153 2204 23 1.4372767857142819 2204 24 -4.977566964285714 2204 25 -11.247321428571418 2204 26 0.6017113095238074 2204 27 1.1519717261904745 2204 28 1.0809523809523824 2204 29 -2.7766741071428527 2204 30 -2.7286086309523796 2204 31 -1.3042782738095227 2204 32 -1.8301711309523725 2204 33 -9.1436755952381 2204 34 -8.962016369047618 2204 35 -0.9959449404761997 2204 36 -4.520944940476184 2204 37 -4.819605654761915 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24 -0.9775669642857139 2205 25 1.7526785714285822 2205 26 -2.8982886904761926 2205 27 0.9019717261904745 2205 28 1.8309523809523824 2205 29 2.9733258928571473 2205 30 1.5213913690476204 2205 31 -3.3042782738095227 2205 32 -2.3301711309523725 2205 33 3.8563244047618994 2205 34 3.5379836309523824 2205 35 2.2540550595238003 2205 36 0.7290550595238159 2205 37 1.930394345238085 2205 38 2.661867559523799 2205 39 3.1959821428571473 2205 40 -2.1878720238095184 2205 41 -3.1932663690476204 2205 42 0.2880580357142861 2205 43 0.11242559523810058 2205 44 2.7264508928571445 2205 45 -0.19095982142856371 2205 46 3.7518601190476133 2205 47 0.6981398809523824 2205 48 2.8807663690476133 2205 49 1.4264508928571402 2205 50 -6.389174107142857 2205 51 -4.224330357142854 1216 1 -2.0129836309523697 1216 2 -5.7876860119047535 1216 3 -2.6341517857142733 1216 4 -6.356696428571425 1216 5 -0.3601934523809476 1216 6 1.5268229166666671 1216 7 -3.5541294642857153 1216 8 0.2429315476190368 1216 9 1.2739583333333258 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1.8182663690476204 1208 17 -2.642819940476194 1208 18 0.6686755952380921 1208 19 -2.862834821428578 1208 20 -2.8495535714285722 1208 21 0.9415550595238003 1208 22 -3.486049107142847 1208 23 3.437276785714282 1208 24 0.2724330357142861 1208 25 2.252678571428582 1208 26 -0.8982886904761926 1208 27 0.9019717261904745 1208 28 -2.9190476190476176 1208 29 0.22332589285714732 1208 30 -4.22860863095238 1208 31 0.6957217261904773 1208 32 4.4198288690476275 1208 33 -2.1436755952381006 1208 34 -1.2120163690476176 1208 35 -2.9959449404761997 1208 36 -0.5209449404761841 1208 37 -5.819605654761915 1208 38 2.661867559523799 1208 39 3.1959821428571473 1208 40 2.0621279761904816 1208 41 2.5567336309523796 1208 42 0.7880580357142861 1208 43 0.8624255952381006 1208 44 -1.0235491071428555 1208 45 1.3090401785714363 1208 46 0.5018601190476133 1208 47 0.6981398809523824 1208 48 0.8807663690476133 1208 49 0.17645089285714022 1208 50 0.36082589285714306 1208 51 3.525669642857146 1209 1 1.5870163690476318 1209 2 -1.3376860119047507 1209 3 -4.284151785714272 1209 4 -2.106696428571425 1209 5 2.6398065476190524 1209 6 -0.5731770833333343 1209 7 2.495870535714282 1209 8 -1.6570684523809618 1209 9 -2.426041666666677 1209 10 -2.5206473214285694 1209 11 -0.8155877976190382 1209 12 1.611532738095228 1209 13 -2.408184523809524 1209 14 1.2344494047619037 1209 15 0.990029761904772 1209 16 2.218266369047619 1209 17 -3.5928199404761934 1209 18 0.2686755952380935 1209 19 -2.2128348214285793 1209 20 1.1004464285714306 1209 21 0.3915550595238031 1209 22 -6.636049107142846 1209 23 0.43727678571428186 1209 24 1.472433035714289 1209 25 -0.6473214285714164 1209 26 1.0517113095238102 1209 27 2.6019717261904773 1209 28 -2.9690476190476147 1209 29 2.0733258928571487 1209 30 -3.2786086309523768 1209 31 0.6457217261904802 1209 32 1.6198288690476303 1209 33 -0.743675595238102 1209 34 1.6379836309523839 1209 35 1.1540550595237988 1209 36 -5.170944940476183 1209 37 -0.06960565476191505 1209 38 0.961867559523796 1209 39 1.8959821428571502 1209 40 2.2621279761904844 1209 41 0.8567336309523768 1209 42 -1.9619419642857139 1209 43 0.962425595238102 1209 44 3.126450892857143 1209 45 -4.190959821428564 1209 46 -1.4481398809523895 1209 47 -1.651860119047619 1209 48 -1.4192336309523839 1209 49 1.126450892857143 1209 50 0.8108258928571459 1209 51 -3.774330357142855 1214 1 -1.7629836309523697 1214 2 -0.7876860119047535 1214 3 1.6158482142857267 1214 4 -2.856696428571425 1214 5 -6.610193452380948 1214 6 -1.2231770833333329 1214 7 0.6958705357142847 1214 8 2.242931547619037 1214 9 0.7739583333333258 1214 10 3.079352678571432 1214 11 3.5844122023809604 1214 12 4.311532738095231 1214 13 1.1918154761904773 1214 14 4.034449404761901 1214 15 2.290029761904769 1214 16 -3.9317336309523796 1214 17 0.35718005952380594 1214 18 -0.08132440476190794 1214 19 -5.362834821428578 1214 20 2.9004464285714278 1214 21 -2.3084449404761997 1214 22 0.763950892857153 1214 23 2.937276785714282 1214 24 2.772433035714286 1214 25 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-0.8103422619047649 1212 34 -4.628683035714285 1212 35 -7.579278273809532 1212 36 -13.437611607142852 1212 37 0.930394345238085 1212 38 2.161867559523799 1212 39 -5.637351190476185 1212 40 -5.2712053571428505 1212 41 -7.2765997023809526 1212 42 0.03805803571428612 1212 43 -1.3042410714285637 1212 44 -7.606882440476188 1212 45 -0.857626488095228 1212 46 -1.2481398809523867 1212 47 -2.718526785714282 1212 48 -14.285900297619055 1212 49 -13.740215773809528 1212 50 -4.722507440476189 1212 51 -6.640997023809522 1213 1 0.9870163690476303 1213 2 0.4623139880952465 1213 3 3.1158482142857267 1213 4 2.893303571428575 1213 5 2.6398065476190524 1213 6 2.026822916666667 1213 7 3.6958705357142847 1213 8 2.742931547619037 1213 9 -1.2260416666666742 1213 10 -0.920647321428568 1213 11 -8.91558779761904 1213 12 2.811532738095231 1213 13 -6.308184523809523 1213 14 -9.4655505952381 1213 15 -3.709970238095231 1213 16 -2.6817336309523796 1213 17 -2.892819940476194 1213 18 -9.831324404761908 1213 19 -2.112834821428578 1213 20 0.9004464285714278 1213 21 -3.3084449404761997 1213 22 -0.236049107142847 1213 23 -9.062723214285718 1213 24 -3.227566964285714 1213 25 -0.2473214285714178 1213 26 -0.14828869047619264 1213 27 3.4019717261904745 1213 28 4.830952380952382 1213 29 0.4733258928571473 1213 30 -8.97860863095238 1213 31 2.4457217261904773 1213 32 0.4198288690476275 1213 33 0.8563244047618994 1213 34 3.0379836309523824 1213 35 -9.7459449404762 1213 36 -2.270944940476184 1213 37 -2.569605654761915 1213 38 2.161867559523799 1213 39 -9.804017857142853 1213 40 -2.4378720238095184 1213 41 4.05673363095238 1213 42 -8.461941964285714 1213 43 -3.1375744047618994 1213 44 3.7264508928571445 1213 45 0.8090401785714363 1213 46 -2.7481398809523867 1213 47 2.9481398809523824 1213 48 -13.119233630952387 1213 49 -2.57354910714286 1213 50 -0.38917410714285694 1213 51 4.025669642857146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 4.0 23 6.0 24 16.0 25 32.0 26 28.0 27 48.0 28 67.0 29 79.0 30 99.0 31 112.0 32 125.0 33 167.0 34 175.0 35 206.0 36 260.0 37 338.0 38 464.0 39 439.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.183795948987246 15.228807201800452 11.740435108777195 37.846961740435106 2 12.673415823022122 20.547431571053618 41.28233970753656 25.4968128983877 3 16.835395575553054 21.447319085114362 36.22047244094488 25.4968128983877 4 13.760779902512185 24.07199100112486 32.508436445444325 29.658792650918635 5 13.873265841769777 33.82077240344957 38.095238095238095 14.210723659542557 6 29.771278590176227 40.86989126359205 16.235470566179227 13.123359580052494 7 25.346831646044244 30.183727034120732 19.49756280464942 24.9718785151856 8 23.13460817397825 40.719910011248594 17.622797150356206 18.522684664416946 9 22.872140982377203 16.835395575553054 17.66029246344207 42.63217097862767 10 13.873265841769777 34.23322084739407 29.92125984251969 21.97225346831646 11 31.158605174353205 22.42219722534683 28.83389576302962 17.585301837270343 12 20.92238470191226 23.322084739407575 26.959130108736407 28.79640044994376 13 24.559430071241096 21.25984251968504 31.008623922009747 23.172103487064117 14 20.50993625796775 20.359955005624297 24.70941132358455 34.4206974128234 15 22.42219722534683 25.909261342332208 21.934758155230597 29.733783277090364 16 29.92125984251969 27.334083239595053 22.347206599175102 20.39745031871016 17 21.672290963629546 34.758155230596174 22.947131608548933 20.622422197225347 18 29.621297337832768 26.621672290963627 24.634420697412825 19.122609673790777 19 24.821897262842143 23.809523809523807 23.097112860892388 28.271466066741656 20 22.347206599175102 25.196850393700785 22.534683164604424 29.92125984251969 21 21.484814398200225 24.9718785151856 25.084364454443193 28.45894263217098 22 22.947131608548933 32.62092238470191 23.20959880014998 21.222347206599178 23 21.97225346831646 24.85939257592801 24.371953505811774 28.79640044994376 24 28.49643794525684 24.9718785151856 24.746906636670417 21.784776902887142 25 22.797150356205474 32.470941132358455 22.684664416947882 22.04724409448819 26 27.85901762279715 26.621672290963627 24.371953505811774 21.147356580427445 27 22.122234720659918 32.73340832395951 24.146981627296586 20.99737532808399 28 22.609673790776153 25.271841019872515 22.609673790776153 29.50881139857518 29 24.221972253468316 26.396700412448443 27.071616047994002 22.30971128608924 30 23.62204724409449 28.98387701537308 25.12185976752906 22.272215973003377 31 23.809523809523807 25.196850393700785 25.084364454443193 25.909261342332208 32 24.634420697412825 26.09673790776153 24.78440194975628 24.484439445069366 33 21.597300337457817 25.909261342332208 29.09636295463067 23.397075365579305 34 24.25946756655418 25.12185976752906 26.284214473190854 24.334458192725908 35 24.78440194975628 26.584176977877767 25.271841019872515 23.35958005249344 36 21.372328458942633 26.546681664791898 29.92125984251969 22.15973003374578 37 19.68503937007874 28.90888638920135 27.896512935883017 23.509561304836897 38 19.310086239220098 21.484814398200225 35.32058492688414 23.88451443569554 39 19.122609673790777 20.959880014998124 28.946381702287216 30.971128608923888 40 18.747656542932134 21.63479565054368 36.97037870266217 22.647169103862016 41 20.172478440194975 18.972628421447318 29.88376452943382 30.971128608923888 42 26.284214473190854 20.73490813648294 29.733783277090364 23.247094113235846 43 16.572928383952007 21.297337832770904 37.79527559055118 24.334458192725908 44 15.860517435320585 28.421447319085114 31.2710911136108 24.446944131983503 45 15.485564304461944 22.15973003374578 39.82002249718785 22.534683164604424 46 23.73453318335208 20.434945631796026 30.783652043494563 25.04686914135733 47 14.810648668916384 22.722159730033745 30.33370828646419 32.133483314585675 48 14.510686164229472 22.272215973003377 30.25871766029246 32.95838020247469 49 21.559805024371954 23.73453318335208 29.92125984251969 24.78440194975628 50 13.910761154855644 22.647169103862016 37.75778027746532 25.684289463817024 51 15.6355455568054 22.947131608548933 28.83389576302962 32.58342707161605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 2.0 13 1.0 14 0.0 15 0.0 16 0.0 17 1.0 18 2.0 19 1.0 20 0.0 21 0.0 22 0.0 23 1.0 24 2.0 25 2.0 26 3.5 27 5.0 28 10.0 29 15.0 30 21.0 31 27.0 32 46.0 33 65.0 34 75.5 35 86.0 36 110.5 37 135.0 38 144.5 39 154.0 40 170.5 41 187.0 42 188.5 43 190.0 44 225.0 45 260.0 46 269.5 47 279.0 48 276.0 49 273.0 50 274.0 51 275.0 52 289.5 53 304.0 54 234.5 55 165.0 56 130.0 57 95.0 58 83.0 59 71.0 60 47.5 61 24.0 62 18.5 63 13.0 64 13.5 65 14.0 66 9.0 67 4.0 68 6.0 69 8.0 70 4.5 71 1.0 72 0.5 73 0.0 74 1.5 75 2.0 76 1.0 77 1.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03749531308586427 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2667.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.31346081739783 #Duplication Level Percentage of deduplicated Percentage of total 1 99.1876523151909 91.56355455568054 2 0.36555645816409427 0.6749156355455568 3 0.08123476848090982 0.22497187851518563 4 0.12185215272136475 0.44994375703037126 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.20308692120227456 4.949381327334083 >50 0.04061738424045491 2.1372328458942635 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 57 2.1372328458942635 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 38 1.4248218972628421 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 35 1.3123359580052494 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 29 1.0873640794900636 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 17 0.6374203224596925 No Hit CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC 13 0.4874390701162355 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 4 0.14998125234345708 No Hit TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 4 0.14998125234345708 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 4 0.14998125234345708 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 3 0.11248593925759282 Illumina PCR Primer Index 8 (95% over 21bp) TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT 3 0.11248593925759282 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.03749531308586427 22 0.0 0.0 0.03749531308586427 23 0.0 0.0 0.07499062617172854 24 0.0 0.0 0.14998125234345708 25 0.0 0.0 0.14998125234345708 26 0.0 0.0 0.2249718785151856 27 0.0 0.0 0.3374578177727784 28 0.0 0.0 0.5249343832020997 29 0.0 0.0 0.9373828271466067 30 0.0 0.0 1.5748031496062993 31 0.0 0.0 2.6246719160104988 32 0.0 0.0 4.2369703787026625 33 0.0 0.0 5.399325084364454 34 0.0 0.0 6.974128233970753 35 0.0 0.0 8.361454818147731 36 0.0 0.0 9.936257967754031 37 0.0 0.0 12.335958005249344 38 0.0 0.0 14.960629921259843 39 0.0 0.0 17.66029246344207 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCCG 30 0.0042843227 30.000002 10 TGCCGTC 30 0.0042843227 30.000002 45 CGAGCCC 30 0.0042843227 30.000002 15 CCGAGCC 30 0.0042843227 30.000002 14 TCTCCGA 30 0.0042843227 30.000002 11 TCCGAGC 30 0.0042843227 30.000002 13 CTCCGAG 30 0.0042843227 30.000002 12 AGCCCAC 35 0.009106225 25.714285 17 GTATGCC 35 0.009106225 25.714285 42 ATCTCGT 35 0.009106225 25.714285 37 GCCCACG 35 0.009106225 25.714285 18 CCACGAG 35 0.009106225 25.714285 20 TATGCCG 35 0.009106225 25.714285 43 CATCTCC 35 0.009106225 25.714285 9 CACGAGA 35 0.009106225 25.714285 21 CCCACGA 35 0.009106225 25.714285 19 TCTCGTA 35 0.009106225 25.714285 38 ACGAGAC 35 0.009106225 25.714285 22 ATGCCGT 35 0.009106225 25.714285 44 CGTATGC 35 0.009106225 25.714285 41 >>END_MODULE