FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_60.34100000004bd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_60.34100000004bd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC572.1372328458942635No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC381.4248218972628421No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC351.3123359580052494No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC291.0873640794900636No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.6374203224596925No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC130.4874390701162355No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC40.14998125234345708No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT40.14998125234345708No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC40.14998125234345708No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT30.11248593925759282Illumina PCR Primer Index 8 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT30.11248593925759282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG300.004284322730.00000210
TGCCGTC300.004284322730.00000245
CGAGCCC300.004284322730.00000215
CCGAGCC300.004284322730.00000214
TCTCCGA300.004284322730.00000211
TCCGAGC300.004284322730.00000213
CTCCGAG300.004284322730.00000212
AGCCCAC350.00910622525.71428517
GTATGCC350.00910622525.71428542
ATCTCGT350.00910622525.71428537
GCCCACG350.00910622525.71428518
CCACGAG350.00910622525.71428520
TATGCCG350.00910622525.71428543
CATCTCC350.00910622525.7142859
CACGAGA350.00910622525.71428521
CCCACGA350.00910622525.71428519
TCTCGTA350.00910622525.71428538
ACGAGAC350.00910622525.71428522
ATGCCGT350.00910622525.71428544
CGTATGC350.00910622525.71428541
Produced by FastQC (version 0.11.2)