FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_6.341000000047d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_6.341000000047d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573522
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTT401.5586504E-533.75176630
TGCGTTA406.1636034E-428.1240231
GGTTACG801.0513395E-725.3138269
CGGTACT2050.024.14760630
TACGTTT952.1114829E-823.68338412
AGTATTG2100.023.572667
TTGCGGT2050.023.04998627
GTATAAT903.2710705E-722.5070651
GCGTTAT500.002263696222.49921632
CGTTATC500.002263696222.49921633
TATGCCG702.7006714E-522.49921443
GCGGTAC2150.021.97789429
ATATGAG1156.7539077E-921.520993
TGCGGTA2200.021.47839728
CCTTGCG2400.020.6260825
CTTAATA1109.965879E-820.4538332
GGTACTA2500.019.79930931
CACTATA807.536353E-519.6936821
CTCGTAT807.556589E-519.68681339
TATATGA1151.5924525E-719.5645372
Produced by FastQC (version 0.11.2)