FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_59.34100000004bc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_59.34100000004bc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC371.0787172011661808No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC361.0495626822157436No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC310.9037900874635568No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC230.6705539358600583No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC220.641399416909621No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC120.3498542274052478No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC110.3206997084548105TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC80.23323615160349853No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.20408163265306123No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC60.1749271137026239No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT40.11661807580174927Illumina PCR Primer Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC206.086404E-445.045
AGCCCAC250.001825857436.017
GTATGCC250.001825857436.042
ATCTCGT250.001825857436.037
ATCTCCG250.001825857436.010
GCCCACG250.001825857436.018
CCACGAG250.001825857436.020
TATGCCG250.001825857436.043
CATCTCC250.001825857436.09
GAGCCCA250.001825857436.016
CACGAGA250.001825857436.021
CCCACGA250.001825857436.019
TCTCGTA250.001825857436.038
ACGAGAC250.001825857436.022
ATGCCGT250.001825857436.044
CGTATGC250.001825857436.041
CGAGCCC250.001825857436.015
CCGAGCC250.001825857436.014
TCGTATG250.001825857436.040
TCTCCGA250.001825857436.011
Produced by FastQC (version 0.11.2)