FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_58.34100000004bb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_58.34100000004bb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648997
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.033.946221
ATCTATA300.005145756529.9998442
TATGTAA602.4163455E-729.9998442
TAAGGTA406.1634125E-428.1248554
GTGGTAT1950.026.5444621
GTATCAA21300.026.5200791
ATATGTA801.0500116E-725.3182221
TGGTATC2150.024.0696452
GCGAATT500.002263623322.49988413
TGGACCG500.002263623322.4998845
TATGCCG602.4659897E-422.49988443
TGTGCGA903.28253E-722.49988410
TACGCCC500.002263623322.4998844
GGACCGT500.002263623322.4998846
GTGCGAA500.002263623322.49988411
CTGTGCG1056.097798E-821.4284619
TATCAAC26800.020.9047432
ACTAATC654.2356373E-420.76912522
TCTTACG550.00393483720.454448
TACTACT550.00393483720.454444
Produced by FastQC (version 0.11.2)