FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_57.34100000004b9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_57.34100000004b9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331909
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC6110.18408660205056204No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5170.15576558635047558No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4230.1274445706503891No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT3970.11961109822270562No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3490.10514930297159764No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT3420.10304029116414438No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3320.10002741715349689No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAC250.002099114236.0203821
TATGTAA401.55763E-533.7487562
CGGGTAG300.005142972329.99889629
TTGTGTA300.005142972329.9988964
ATGTACA300.005142972329.9988964
GGGCGCA300.005142972329.99889620
TGAGCCG300.005142972329.99889610
CTCTAGA300.005142972329.9988962
ATATGTA554.134952E-628.6525751
GGGGCGA406.1527325E-428.128218
TGTAAGT2750.027.8339271
CGGGGCC507.1377915E-527.00307517
GATAGGA608.170962E-626.24903333
TACATTG709.277119E-725.7133392
AATATGC901.1850716E-824.9990795
AGATTTT1101.5097612E-1024.54824818
GCGCGAC551.3656828E-424.5445520
GTACATT651.5128639E-524.2444861
AAGCGAG751.6921294E-623.99911527
TAATATG851.8858918E-723.822654
Produced by FastQC (version 0.11.2)