FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_55.34100000004b73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_55.34100000004b73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421106
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC24410.5796640275845036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG2500.037.799736
TATGCCG2550.037.05853343
ATCGTGT2650.035.66009530
ACGATCG2700.034.99972527
CTCGTAT2800.034.55329539
CGTATGC2750.034.3633641
TCGTATG2750.034.3633640
TGCCGTC2750.034.3633645
CGAGACG2850.033.94709823
CGATCGT2850.033.94709828
GTGTATC2800.033.7497333
CCCACGA2900.033.36180519
TCGTGTA2850.033.1576331
CGTGTAT2850.033.1576332
GATCGTG2900.032.5859529
CACGAGA3050.032.4587621
AGACGAT2950.032.03364625
ATGCCGT2950.032.03364644
GCCCACG3000.031.49975218
GACGATC3000.031.49975226
Produced by FastQC (version 0.11.2)