FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_54.34100000004b66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_54.34100000004b66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621611
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG801.0004442E-1030.93716823
ATCGTGT751.7007551E-929.99967830
CGATCGT702.8820068E-828.9282628
TATGCCG803.3887773E-928.12469743
CTCGTAT903.947207E-1027.49970639
CGACCCG755.6310455E-826.999715
CGCCTAA608.184319E-626.2497222
CGAACGT709.296891E-725.7140084
TCGAACG709.296891E-725.7140083
CGTGTAT801.0522672E-725.31222732
GCCCACG901.1888915E-824.99973318
GTAAGTA751.6945287E-624.0016732
TCTCGTA952.1116648E-823.68395838
TGCCGTC952.1116648E-823.68395845
TCGTGTA903.2822572E-722.4997631
ATTCGAA955.502534E-721.3241391
ACGATCG955.5238706E-721.31556127
ACGCCTA855.0265426E-621.17624321
TAGCCTA754.5986486E-520.9997755
GCACTAT550.00392562120.4625571
Produced by FastQC (version 0.11.2)