FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_53.34100000004b5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_53.34100000004b5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548630
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGT552.744855E-936.81624230
ACCGTCG300.005146265529.9984198
CTCGTAT852.0372681E-1029.11611239
CGAGACG852.0372681E-1029.11611223
GTATCAA9100.025.7246551
TCGTATG1001.3424142E-924.74869740
TCGTGTA952.1114829E-823.68296431
ACGATCG903.2821117E-722.49881627
CGTGTAT903.2821117E-722.49881632
CGATCGT903.2821117E-722.49881628
TATGCCG1104.0436134E-922.49881443
TATCTCG1104.0436134E-922.49881436
TCTCGTA1104.0436134E-922.49881438
GTGTATC1056.096525E-821.42744433
ACGGGAT955.509137E-721.32049816
GATCGTG955.5236524E-721.31466929
GAGACGA1251.7633283E-819.79895624
ATCAACG11750.019.7223663
TATCAAC11550.019.6742882
TCAACGC11550.019.6742884
Produced by FastQC (version 0.11.2)