FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_52.34100000004b4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_52.34100000004b4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555138
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG352.818577E-432.142735
CGCCTAA352.818577E-432.1427322
ACGGGAT1058.367351E-1125.71418216
GGCTTAG802.9656185E-622.5039631
TATGCCG602.4653462E-422.4999143
AAATAGT500.00226319922.49990814
GGTATCA8150.021.8136581
GTATCAA13850.021.447821
GTATAGG654.229396E-420.772891
CTACGCC654.2345427E-420.7691483
TATTCTC654.2345427E-420.7691485
TCGTATG654.2345427E-420.76914840
CCTTACG550.003934104520.4544642
ACGCCTA550.003934104520.45446421
TCCTATT908.238918E-619.999922
TTATTCT807.55424E-519.6874224
CCTATTC706.9774326E-419.2856373
TTAGGTA600.006506503618.74992631
TCTCCGA3250.018.69223445
GTCTATG851.20305434E-418.5293392
Produced by FastQC (version 0.11.2)