FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_51.34100000004b30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_51.34100000004b30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589892
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG352.8190037E-432.1423154
TAACGCA300.00514567729.9994934
ATAACGC406.163167E-428.1245233
TCGTGTG450.001225968124.9995755
GAACGGC651.5223175E-524.2303586
CGGGGTA851.8907122E-723.82312617
CGCCTAA952.111301E-823.6838122
ATCGTAG500.002263535522.4996233
CGTCTAT1501.4551915E-1121.0032041
TATGCCG754.5983572E-520.99964543
TCTCCGA6850.020.03615645
GTCTAAA908.229212E-620.0030521
ACGCCTA1251.7633283E-819.79966421
TAGAGTA807.5560136E-519.6871665
CTCGTAT807.5560136E-519.68716639
ACGGGGT4100.019.2086216
CATCTCC23850.019.1506245
ATCGTGT951.3142506E-518.94704830
GAGACGA951.3142506E-518.94704824
CGGGGTG2750.018.81786317
Produced by FastQC (version 0.11.2)