FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_50.34100000004b23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_50.34100000004b23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436939
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAG300.005143936329.9998476
CGCCTAA952.3646862E-1128.42090822
CGCAATG406.1601604E-428.12485718
ACGCCTA1058.367351E-1125.71415321
ATAATAC450.00122537424.9998723
TTAGAGT702.6981468E-522.4998874
CGAGACG702.6981468E-522.49988723
TCGCAAT500.002262447522.49988617
AAACGCC1156.7411747E-921.5216319
GGATAAT550.00392759820.4591241
ACCTATA550.00392759820.4591241
TATGCCG908.232404E-619.99989943
AATACTA908.232404E-619.9998995
GCCTAAT1251.7598722E-819.799923
AACCGTC706.9742266E-419.28561834
ATACTAT706.9742266E-419.2856186
CGTGTAT706.9742266E-419.28561832
AAAACGC1302.7606802E-819.03836418
CTAATCA1302.7606802E-819.03836425
ATGCCGT951.3128421E-518.94727144
Produced by FastQC (version 0.11.2)