FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_49.34100000004b09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_49.34100000004b09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248654
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC3460.13914917918070893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTG300.005139930629.999623
CCCCTGA406.131249E-428.1415981
TGCCGTC507.136891E-526.9996445
TATGCCG500.002259835422.499743
CCCACGA602.4601957E-422.499719
GGAGTAG500.002259835422.499731
CGTGTAT500.002259835422.499732
AGTGTTA500.002259835422.49976
TATCTCG654.225755E-420.76895336
TACAAGG807.533388E-519.6872352
GGTATCA4250.019.5997961
GCCCACG706.963056E-419.28545618
CCACGAG706.963056E-419.28545620
ACGGGAG1302.746674E-819.03820816
GTATCAA7250.018.9422071
ACGGGCA600.006496990618.7497517
CGGGCTG600.006496990618.7497517
ATGCCGT600.006496990618.7497544
AGGGCGT600.006496990618.7497510
CTCGTAT750.001106999917.99975841
Produced by FastQC (version 0.11.2)