##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 6h_47.34100000004aee.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2719
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.9536594336153	38.0	32.0	38.0	32.0	38.0
2	35.17727105553512	38.0	32.0	38.0	32.0	38.0
3	35.24714968738507	38.0	32.0	38.0	32.0	38.0
4	35.439499816108864	38.0	32.0	38.0	32.0	38.0
5	35.50422949613829	38.0	32.0	38.0	32.0	38.0
6	36.93563810224347	38.0	38.0	40.0	32.0	40.0
7	36.523354174328794	38.0	38.0	40.0	32.0	40.0
8	36.7219566016918	38.0	38.0	40.0	32.0	40.0
9	36.8477381390217	38.0	38.0	40.0	32.0	40.0
10	36.818683339463036	38.0	38.0	40.0	32.0	40.0
11	36.867966164030896	38.0	38.0	40.0	32.0	40.0
12	36.400882677454945	38.0	38.0	40.0	32.0	40.0
13	36.67046708348658	38.0	38.0	40.0	32.0	40.0
14	36.469290180213314	38.0	38.0	40.0	32.0	40.0
15	36.43324751746966	38.0	38.0	40.0	32.0	40.0
16	36.15336520779699	38.0	38.0	40.0	27.0	40.0
17	36.28760573740346	38.0	38.0	40.0	32.0	40.0
18	36.23501287237955	38.0	38.0	40.0	27.0	40.0
19	36.15557190143435	38.0	38.0	40.0	32.0	40.0
20	36.272894446487676	38.0	38.0	40.0	32.0	40.0
21	36.34718646561236	38.0	38.0	40.0	32.0	40.0
22	36.54836336888562	38.0	38.0	40.0	32.0	40.0
23	36.53843324751747	38.0	38.0	40.0	32.0	40.0
24	36.38470025744759	38.0	38.0	40.0	32.0	40.0
25	36.083118793674146	38.0	38.0	40.0	27.0	40.0
26	36.105185730047815	38.0	38.0	40.0	27.0	40.0
27	36.187936741449064	38.0	38.0	40.0	27.0	40.0
28	36.132033835969104	38.0	38.0	40.0	27.0	40.0
29	36.235380654652445	38.0	38.0	40.0	32.0	40.0
30	35.84920926811328	38.0	38.0	40.0	27.0	40.0
31	35.91320338359691	38.0	38.0	40.0	27.0	40.0
32	35.71864656123575	38.0	38.0	40.0	27.0	40.0
33	35.7616770871644	38.0	38.0	40.0	27.0	40.0
34	35.72968002942258	38.0	38.0	40.0	27.0	40.0
35	35.85288709084222	38.0	38.0	40.0	27.0	40.0
36	35.62633321073924	38.0	38.0	40.0	27.0	40.0
37	35.374402353806545	38.0	38.0	40.0	27.0	40.0
38	35.459360058845164	38.0	38.0	40.0	27.0	40.0
39	35.23464509010666	38.0	32.0	40.0	27.0	40.0
40	35.206693637366676	38.0	32.0	40.0	27.0	40.0
41	34.798823096726736	38.0	32.0	40.0	13.0	40.0
42	34.824200073556455	38.0	32.0	40.0	13.0	40.0
43	35.14711290915778	38.0	32.0	40.0	27.0	40.0
44	34.84442809856565	38.0	32.0	40.0	27.0	40.0
45	34.97535858771607	38.0	32.0	40.0	27.0	40.0
46	34.7815373299007	38.0	32.0	40.0	13.0	40.0
47	34.727105553512324	38.0	32.0	40.0	13.0	40.0
48	34.52041191614564	38.0	32.0	40.0	13.0	40.0
49	34.46156675248253	38.0	32.0	40.0	13.0	40.0
50	34.59801397572637	38.0	32.0	40.0	13.0	40.0
51	33.85325487311511	38.0	32.0	40.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	-4.8634300595238
2201	2	-0.17101934523808637
2201	3	-5.041629464285705
2201	4	1.4376860119047734
2201	5	1.003683035714289
2201	6	-0.2700148809523739
2201	7	-6.0179687500000085
2201	8	-7.165736607142868
2201	9	1.5026041666666643
2201	10	-0.10543154761904105
2201	11	-4.036160714285721
2201	12	1.1259300595238102
2201	13	2.732142857142854
2201	14	-2.758816964285714
2201	15	0.007663690476185536
2201	16	2.394084821428571
2201	17	1.8188616071428498
2201	18	1.243154761904762
2201	19	3.134486607142861
2201	20	1.5861235119047663
2201	21	2.0802455357142833
2201	22	2.480245535714282
2201	23	0.32369791666666714
2201	24	1.1683779761904702
2201	25	1.1858258928571388
2201	26	3.0848586309523753
2201	27	-2.515922619047622
2201	28	0.7263020833333371
2201	29	0.0948660714285694
2201	30	1.5061383928571388
2201	31	-2.8034226190476303
2201	32	1.8832217261904773
2201	33	-2.9420014880952365
2201	34	0.9771577380952436
2201	35	3.6753720238095298
2201	36	1.4337053571428484
2201	37	2.5919642857142904
2201	38	3.0565476190476204
2201	39	-3.140587797619041
2201	40	0.37180059523809206
2201	41	1.7819196428571402
2201	42	2.069568452380949
2201	43	3.440699404761908
2201	44	-2.412648809523809
2201	45	2.772693452380956
2201	46	1.9275297619047507
2201	47	2.239508928571425
2201	48	-1.390401785714289
2201	49	1.2297991071428598
2201	50	-2.2108258928571374
2201	51	1.1090773809523782
1101	1	-3.3134300595237995
1101	2	1.3789806547619108
1101	3	1.5083705357142918
1101	4	1.7376860119047706
1101	5	0.10368303571429038
1101	6	1.5299851190476232
1101	7	1.13203124999999
1101	8	-2.865736607142871
1101	9	0.6026041666666657
1101	10	0.09456845238096179
1101	11	-0.786160714285721
1101	12	0.32593005952381304
1101	13	0.8321428571428555
1101	14	-3.4088169642857125
1101	15	-1.4423363095238173
1101	16	1.0940848214285737
1101	17	-3.2811383928571516
1101	18	-6.256845238095238
1101	19	-5.365513392857139
1101	20	-4.713876488095231
1101	21	-7.619754464285716
1101	22	-2.3197544642857153
1101	23	1.7236979166666657
1101	24	1.9683779761904674
1101	25	-0.4141741071428626
1101	26	-3.215141369047622
1101	27	-0.7659226190476218
1101	28	0.17630208333333997
1101	29	-0.20513392857142776
1101	30	-1.693861607142864
1101	31	0.8465773809523682
1101	32	0.8832217261904773
1101	33	2.0079985119047663
1101	34	2.3771577380952422
1101	35	1.8753720238095326
1101	36	3.2337053571428456
1101	37	0.09196428571429038
1101	38	0.7565476190476232
1101	39	1.609412202380959
1101	40	1.4718005952380935
1101	41	-1.8180803571428612
1101	42	2.269568452380952
1101	43	3.740699404761905
1101	44	2.037351190476194
1101	45	1.8726934523809575
1101	46	1.1275297619047535
1101	47	2.8395089285714263
1101	48	3.059598214285714
1101	49	2.1797991071428626
1101	50	3.33917410714286
1101	51	4.159077380952375
2202	1	2.8865699404761997
2202	2	2.5789806547619136
2202	3	0.7083705357142946
2202	4	-1.0623139880952266
2202	5	2.503683035714289
2202	6	1.3966517857142904
2202	7	2.065364583333327
2202	8	0.6675967261904674
2202	9	0.3359375
2202	10	0.22790178571429465
2202	11	1.8805059523809433
2202	12	2.459263392857146
2202	13	2.56547619047619
2202	14	2.6578497023809504
2202	15	3.0909970238095212
2202	16	1.2274181547619065
2202	17	1.9855282738095141
2202	18	1.0764880952380977
2202	19	0.9678199404761969
2202	20	2.419456845238102
2202	21	2.2469122023809476
2202	22	2.146912202380946
2202	23	2.657031250000003
2202	24	2.501711309523806
2202	25	2.852492559523803
2202	26	3.2515252976190396
2202	27	3.567410714285714
2202	28	2.6429687500000014
2202	29	2.2615327380952337
2202	30	1.172805059523803
2202	31	-0.886755952380966
2202	32	0.8832217261904773
2202	33	-4.858668154761904
2202	34	-5.689508928571424
2202	35	0.34203869047619406
2202	36	-5.232961309523819
2202	37	-1.9080357142857096
2202	38	1.2232142857142847
2202	39	-4.390587797619041
2202	40	-4.128199404761908
2202	41	0.4485863095238045
2202	42	-3.930431547619051
2202	43	-4.72596726190476
2202	44	-0.16264880952380878
2202	45	0.2726934523809561
2202	46	-5.4058035714285815
2202	47	0.23950892857142492
2202	48	-6.473735119047621
2202	49	-2.6868675595238045
2202	50	-0.4608258928571374
2202	51	0.3590773809523782
1102	1	0.48656994047620117
1102	2	2.5789806547619136
1102	3	1.5083705357142918
1102	4	1.7376860119047706
1102	5	-1.0963169642857125
1102	6	0.7299851190476261
1102	7	-0.6679687500000071
1102	8	1.3342633928571317
1102	9	1.8026041666666615
1102	10	2.094568452380962
1102	11	2.413839285714282
1102	12	2.325930059523813
1102	13	1.232142857142854
1102	14	2.7911830357142833
1102	15	2.9576636904761884
1102	16	3.0940848214285737
1102	17	2.9188616071428513
1102	18	1.743154761904762
1102	19	1.2344866071428626
1102	20	2.286123511904769
1102	21	1.5802455357142833
1102	22	2.6802455357142847
1102	23	2.9236979166666686
1102	24	1.1683779761904702
1102	25	2.185825892857139
1102	26	2.984858630952374
1102	27	1.2340773809523782
1102	28	3.17630208333334
1102	29	2.7948660714285722
1102	30	3.7061383928571416
1102	31	3.246577380952367
1102	32	4.08322172619048
1102	33	3.6079985119047606
1102	34	3.577157738095245
1102	35	3.8753720238095326
1102	36	3.6337053571428513
1102	37	4.291964285714293
1102	38	4.156547619047622
1102	39	3.0094122023809575
1102	40	2.871800595238092
1102	41	3.981919642857143
1102	42	3.069568452380949
1102	43	3.740699404761905
1102	44	2.037351190476194
1102	45	1.8726934523809575
1102	46	3.727529761904748
1102	47	4.439508928571428
1102	48	5.059598214285714
1102	49	4.179799107142863
1102	50	3.33917410714286
1102	51	3.7590773809523768
2203	1	1.3865699404761997
2203	2	1.0789806547619136
2203	3	2.7083705357142946
2203	4	-0.06231398809522659
2203	5	-1.746316964285711
2203	6	1.229985119047626
2203	7	-1.0179687500000085
2203	8	0.3342633928571317
2203	9	-4.247395833333336
2203	10	-2.355431547619041
2203	11	2.213839285714279
2203	12	2.12593005952381
2203	13	-4.017857142857146
2203	14	0.24118303571428612
2203	15	-4.9923363095238145
2203	16	-3.855915178571429
2203	17	0.31886160714284983
2203	18	2.743154761904762
2203	19	-5.115513392857139
2203	20	0.0861235119047663
2203	21	-0.16975446428571672
2203	22	0.9802455357142819
2203	23	-0.6763020833333329
2203	24	-4.08162202380953
2203	25	3.185825892857139
2203	26	0.08485863095237534
2203	27	0.7340773809523782
2203	28	-0.2736979166666629
2203	29	-3.6551339285714306
2203	30	-3.243861607142861
2203	31	1.9465773809523697
2203	32	1.8832217261904773
2203	33	3.3079985119047635
2203	34	-3.2728422619047564
2203	35	2.1753720238095298
2203	36	-4.316294642857152
2203	37	2.5919642857142904
2203	38	-2.6934523809523796
2203	39	2.109412202380959
2203	40	1.121800595238092
2203	41	0.5319196428571402
2203	42	-5.430431547619051
2203	43	1.190699404761908
2203	44	0.5873511904761912
2203	45	1.022693452380956
2203	46	1.9275297619047507
2203	47	-2.010491071428575
2203	48	-2.890401785714289
2203	49	1.2297991071428598
2203	50	0.7891741071428626
2203	51	-2.390922619047622
1103	1	1.3865699404761997
1103	2	-0.4210193452380864
1103	3	-0.29162946428570535
1103	4	-0.06231398809522659
1103	5	2.503683035714289
1103	6	1.729985119047626
1103	7	1.2320312499999915
1103	8	-0.1657366071428683
1103	9	2.0026041666666643
1103	10	1.894568452380959
1103	11	-0.286160714285721
1103	12	1.1259300595238102
1103	13	0.7321428571428541
1103	14	0.9911830357142861
1103	15	1.2576636904761855
1103	16	-1.8559151785714292
1103	17	-5.93113839285715
1103	18	2.243154761904762
1103	19	2.134486607142861
1103	20	0.0861235119047663
1103	21	2.5802455357142833
1103	22	0.48024553571428186
1103	23	1.8236979166666671
1103	24	-0.33162202380952976
1103	25	-0.0641741071428612
1103	26	-4.665141369047625
1103	27	1.2340773809523782
1103	28	1.4763020833333371
1103	29	-4.155133928571431
1103	30	0.2561383928571388
1103	31	0.19657738095236965
1103	32	0.6332217261904773
1103	33	1.0579985119047635
1103	34	1.4771577380952436
1103	35	-5.32462797619047
1103	36	2.9337053571428484
1103	37	3.5919642857142904
1103	38	1.5565476190476204
1103	39	1.609412202380959
1103	40	1.871800595238092
1103	41	3.78191964285714
1103	42	2.569568452380949
1103	43	1.940699404761908
1103	44	-3.912648809523809
1103	45	2.772693452380956
1103	46	3.9275297619047507
1103	47	3.739508928571425
1103	48	2.859598214285711
1103	49	3.47979910714286
1103	50	3.0391741071428626
1103	51	3.859077380952378
2204	1	0.8865699404761997
2204	2	2.5789806547619136
2204	3	2.7083705357142946
2204	4	0.9376860119047734
2204	5	0.503683035714289
2204	6	2.063318452380962
2204	7	2.065364583333327
2204	8	1.3342633928571317
2204	9	1.6692708333333286
2204	10	2.2279017857142946
2204	11	3.213839285714279
2204	12	3.12593005952381
2204	13	1.8988095238095184
2204	14	2.6578497023809504
2204	15	3.7576636904761855
2204	16	1.8940848214285708
2204	17	2.6521949404761855
2204	18	3.743154761904762
2204	19	3.634486607142861
2204	20	3.0861235119047663
2204	21	2.2469122023809476
2204	22	2.146912202380946
2204	23	-0.009635416666668561
2204	24	2.501711309523806
2204	25	2.852492559523803
2204	26	3.918191964285711
2204	27	3.567410714285714
2204	28	1.3096354166666728
2204	29	2.2615327380952337
2204	30	3.172805059523803
2204	31	3.113244047619034
2204	32	4.883221726190477
2204	33	4.141331845238099
2204	34	3.3104910714285793
2204	35	3.342038690476194
2204	36	3.767038690476184
2204	37	5.09196428571429
2204	38	3.889880952380956
2204	39	3.2760788690476232
2204	40	3.5384672619047564
2204	41	4.115252976190476
2204	42	4.402901785714285
2204	43	4.274032738095244
2204	44	3.5040178571428555
2204	45	5.272693452380956
2204	46	4.260863095238086
2204	47	4.572842261904761
2204	48	4.526264880952375
2204	49	2.97979910714286
2204	50	2.5391741071428626
2204	51	4.692410714285714
2205	1	0.8865699404761997
2205	2	2.5789806547619136
2205	3	2.7083705357142946
2205	4	2.9376860119047734
2205	5	2.503683035714289
2205	6	2.063318452380962
2205	7	2.7320312499999915
2205	8	2.6675967261904674
2205	9	1.6692708333333286
2205	10	2.2279017857142946
2205	11	3.213839285714279
2205	12	3.12593005952381
2205	13	0.5654761904761898
2205	14	3.324516369047622
2205	15	3.0909970238095212
2205	16	3.894084821428571
2205	17	3.31886160714285
2205	18	2.4098214285714263
2205	19	-0.6988467261904745
2205	20	2.419456845238102
2205	21	2.913578869047619
2205	22	3.480245535714282
2205	23	3.323697916666667
2205	24	3.1683779761904702
2205	25	4.185825892857139
2205	26	4.584858630952375
2205	27	-0.09925595238095752
2205	28	1.3096354166666728
2205	29	2.928199404761905
2205	30	3.8394717261904745
2205	31	-5.8867559523809625
2205	32	1.5498883928571416
2205	33	-6.858668154761904
2205	34	-5.689508928571424
2205	35	-2.3246279761904702
2205	36	-7.232961309523819
2205	37	-12.90803571428571
2205	38	-13.44345238095238
2205	39	-7.057254464285709
2205	40	-0.12819940476190794
2205	41	-6.884747023809528
2205	42	-4.597098214285719
2205	43	-4.72596726190476
2205	44	-0.16264880952380878
2205	45	-12.727306547619044
2205	46	-13.07247023809525
2205	47	0.23950892857142492
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1211	27	-5.765922619047622
1211	28	1.17630208333334
1211	29	-5.405133928571431
1211	30	-2.89386160714286
1211	31	-1.1534226190476318
1211	32	-3.9167782738095234
1211	33	-3.9920014880952372
1211	34	-5.622842261904758
1211	35	-5.32462797619047
1211	36	-1.7662946428571544
1211	37	1.6919642857142918
1211	38	-2.2434523809523768
1211	39	0.8094122023809618
1211	40	-1.7281994047619094
1211	41	-0.21808035714285978
1211	42	3.4695684523809476
1211	43	-2.4593005952380906
1211	44	-1.3626488095238116
1211	45	-3.1273065476190425
1211	46	1.1275297619047535
1211	47	-4.760491071428575
1211	48	-3.140401785714289
1211	49	-2.8202008928571374
1211	50	1.7391741071428655
1211	51	0.3590773809523782
1208	1	2.8865699404761997
1208	2	1.0789806547619136
1208	3	1.2083705357142946
1208	4	1.4376860119047734
1208	5	-0.49631696428571104
1208	6	-0.2700148809523739
1208	7	1.7320312499999915
1208	8	1.3342633928571317
1208	9	0.002604166666664298
1208	10	2.394568452380959
1208	11	-5.036160714285721
1208	12	-1.6240699404761898
1208	13	-4.517857142857146
1208	14	-9.508816964285714
1208	15	-4.9923363095238145
1208	16	-4.855915178571429
1208	17	0.8188616071428498
1208	18	-0.00684523809523796
1208	19	-3.615513392857139
1208	20	2.0861235119047663
1208	21	2.5802455357142833
1208	22	0.9802455357142819
1208	23	1.8236979166666671
1208	24	0.16837797619047024
1208	25	3.185825892857139
1208	26	3.5848586309523753
1208	27	1.7340773809523782
1208	28	2.976302083333337
1208	29	-0.4051339285714306
1208	30	-2.743861607142861
1208	31	0.44657738095236965
1208	32	-3.8667782738095227
1208	33	-4.4420014880952365
1208	34	-1.2728422619047564
1208	35	2.1753720238095298
1208	36	3.4337053571428484
1208	37	-8.90803571428571
1208	38	0.8065476190476204
1208	39	0.859412202380959
1208	40	-3.878199404761908
1208	41	-0.4680803571428598
1208	42	-2.180431547619051
1208	43	-3.809300595238092
1208	44	1.0873511904761912
1208	45	-3.977306547619044
1208	46	-2.3224702380952493
1208	47	-2.510491071428575
1208	48	-8.140401785714289
1208	49	-0.2702008928571402
1208	50	-3.2108258928571374
1208	51	4.359077380952378
1209	1	-4.8634300595238
1209	2	-1.6710193452380864
1209	3	-0.29162946428570535
1209	4	1.4376860119047734
1209	5	2.503683035714289
1209	6	0.7299851190476261
1209	7	1.7320312499999915
1209	8	1.3342633928571317
1209	9	2.5026041666666643
1209	10	-4.355431547619041
1209	11	0.21383928571427901
1209	12	0.6259300595238102
1209	13	2.232142857142854
1209	14	-0.2588169642857139
1209	15	3.2576636904761855
1209	16	1.8940848214285708
1209	17	-0.43113839285715017
1209	18	0.49315476190476204
1209	19	1.6344866071428612
1209	20	0.5861235119047663
1209	21	2.0802455357142833
1209	22	1.4802455357142819
1209	23	-3.926302083333333
1209	24	-1.0816220238095298
1209	25	-2.564174107142861
1209	26	2.5848586309523753
1209	27	2.234077380952378
1209	28	1.4763020833333371
1209	29	-3.1551339285714306
1209	30	2.506138392857139
1209	31	1.1965773809523697
1209	32	-2.3667782738095227
1209	33	0.5579985119047635
1209	34	1.4771577380952436
1209	35	2.6753720238095298
1209	36	2.4337053571428484
1209	37	-1.6580357142857096
1209	38	-2.6934523809523796
1209	39	1.359412202380959
1209	40	2.371800595238092
1209	41	3.28191964285714
1209	42	2.069568452380949
1209	43	-1.809300595238092
1209	44	1.0873511904761912
1209	45	4.272693452380956
1209	46	3.9275297619047507
1209	47	4.739508928571425
1209	48	-0.890401785714289
1209	49	-2.27020089285714
1209	50	3.5391741071428626
1209	51	2.109077380952378
1214	1	1.3865699404761997
1214	2	2.5789806547619136
1214	3	-1.7916294642857054
1214	4	-0.06231398809522659
1214	5	1.003683035714289
1214	6	-1.770014880952374
1214	7	0.7320312499999915
1214	8	-6.415736607142868
1214	9	-2.7473958333333357
1214	10	-1.855431547619041
1214	11	0.21383928571427901
1214	12	-0.3740699404761898
1214	13	-6.017857142857146
1214	14	-0.2588169642857139
1214	15	-6.7423363095238145
1214	16	-1.1059151785714292
1214	17	0.31886160714284983
1214	18	-6.256845238095238
1214	19	-0.6155133928571388
1214	20	-5.163876488095234
1214	21	-4.169754464285717
1214	22	-7.769754464285718
1214	23	-4.426302083333333
1214	24	-3.0816220238095298
1214	25	-3.564174107142861
1214	26	1.0848586309523753
1214	27	0.7340773809523782
1214	28	0.4763020833333371
1214	29	2.5948660714285694
1214	30	2.506138392857139
1214	31	2.4465773809523697
1214	32	3.3832217261904773
1214	33	1.3079985119047635
1214	34	1.9771577380952436
1214	35	-2.5746279761904702
1214	36	-3.3162946428571516
1214	37	0.09196428571429038
1214	38	3.5565476190476204
1214	39	0.35941220238095895
1214	40	2.371800595238092
1214	41	2.28191964285714
1214	42	-2.680431547619051
1214	43	0.6906994047619079
1214	44	-4.912648809523809
1214	45	1.772693452380956
1214	46	1.4275297619047507
1214	47	1.739508928571425
1214	48	3.859598214285711
1214	49	3.47979910714286
1214	50	2.5391741071428626
1214	51	3.859077380952378
1215	1	-0.11343005952380025
1215	2	1.0789806547619136
1215	3	-0.29162946428570535
1215	4	-0.06231398809522659
1215	5	-3.746316964285711
1215	6	1.729985119047626
1215	7	1.7320312499999915
1215	8	0.3342633928571317
1215	9	2.0026041666666643
1215	10	0.894568452380959
1215	11	1.713839285714279
1215	12	1.6259300595238102
1215	13	-1.267857142857146
1215	14	2.991183035714286
1215	15	-0.7423363095238145
1215	16	2.394084821428571
1215	17	1.8188616071428498
1215	18	-2.256845238095238
1215	19	2.134486607142861
1215	20	-1.1638764880952337
1215	21	-4.169754464285717
1215	22	-1.7697544642857181
1215	23	0.32369791666666714
1215	24	0.6683779761904702
1215	25	-3.064174107142861
1215	26	-2.6651413690476247
1215	27	2.234077380952378
1215	28	0.9763020833333371
1215	29	1.0948660714285694
1215	30	2.006138392857139
1215	31	3.4465773809523697
1215	32	2.3832217261904773
1215	33	3.3079985119047635
1215	34	3.9771577380952436
1215	35	2.1753720238095298
1215	36	3.4337053571428484
1215	37	2.5919642857142904
1215	38	-2.1934523809523796
1215	39	-2.640587797619041
1215	40	1.121800595238092
1215	41	-2.96808035714286
1215	42	-2.680431547619051
1215	43	-2.809300595238092
1215	44	1.0873511904761912
1215	45	-1.977306547619044
1215	46	-2.3224702380952493
1215	47	1.489508928571425
1215	48	-1.390401785714289
1215	49	-3.77020089285714
1215	50	0.2891741071428626
1215	51	0.8590773809523782
1212	1	2.8865699404761997
1212	2	0.5789806547619136
1212	3	0.7083705357142946
1212	4	2.9376860119047734
1212	5	2.503683035714289
1212	6	2.729985119047626
1212	7	2.065364583333327
1212	8	0.6675967261904674
1212	9	2.3359375
1212	10	2.2279017857142946
1212	11	2.5471726190476147
1212	12	2.459263392857146
1212	13	2.56547619047619
1212	14	3.324516369047622
1212	15	3.0909970238095212
1212	16	3.2274181547619065
1212	17	2.6521949404761855
1212	18	3.0764880952380977
1212	19	2.967819940476197
1212	20	2.419456845238102
1212	21	2.913578869047619
1212	22	2.8135788690476176
1212	23	2.657031250000003
1212	24	2.501711309523806
1212	25	3.5191592261904745
1212	26	3.918191964285711
1212	27	3.567410714285714
1212	28	3.976302083333337
1212	29	2.928199404761905
1212	30	3.8394717261904745
1212	31	3.7799107142857054
1212	32	4.216555059523813
1212	33	4.141331845238099
1212	34	3.9771577380952436
1212	35	4.67537202380953
1212	36	3.767038690476184
1212	37	4.425297619047626
1212	38	3.889880952380956
1212	39	3.9427455357142946
1212	40	3.5384672619047564
1212	41	4.115252976190476
1212	42	4.402901785714285
1212	43	3.6073660714285722
1212	44	4.170684523809527
1212	45	4.606026785714292
1212	46	4.927529761904751
1212	47	5.239508928571425
1212	48	5.192931547619047
1212	49	4.3131324404761955
1212	50	3.8725074404761983
1212	51	2.692410714285714
1213	1	-0.11343005952380025
1213	2	2.5789806547619136
1213	3	-0.29162946428570535
1213	4	-0.06231398809522659
1213	5	-0.49631696428571104
1213	6	1.729985119047626
1213	7	1.7320312499999915
1213	8	2.3342633928571317
1213	9	2.0026041666666643
1213	10	0.894568452380959
1213	11	1.213839285714279
1213	12	-1.8740699404761898
1213	13	-14.267857142857146
1213	14	-6.508816964285714
1213	15	-4.2423363095238145
1213	16	-13.60591517857143
1213	17	1.3188616071428498
1213	18	-1.256845238095238
1213	19	-4.365513392857139
1213	20	-11.413876488095234
1213	21	-13.919754464285717
1213	22	-11.019754464285718
1213	23	-1.6763020833333329
1213	24	-1.8316220238095298
1213	25	-13.314174107142861
1213	26	-2.9151413690476247
1213	27	-3.265922619047622
1213	28	-1.0236979166666629
1213	29	1.5948660714285694
1213	30	2.506138392857139
1213	31	2.4465773809523697
1213	32	-0.11677827380952266
1213	33	-0.19200148809523654
1213	34	1.9771577380952436
1213	35	-22.32462797619047
1213	36	-22.56629464285715
1213	37	-12.40803571428571
1213	38	-2.9434523809523796
1213	39	-3.390587797619041
1213	40	-5.628199404761908
1213	41	2.78191964285714
1213	42	-2.430431547619051
1213	43	2.940699404761908
1213	44	2.837351190476191
1213	45	-12.227306547619044
1213	46	-5.572470238095249
1213	47	-12.260491071428575
1213	48	-4.640401785714289
1213	49	-2.52020089285714
1213	50	2.5391741071428626
1213	51	-2.140922619047622
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	11.0
24	13.0
25	23.0
26	32.0
27	48.0
28	72.0
29	95.0
30	96.0
31	98.0
32	142.0
33	145.0
34	168.0
35	171.0
36	208.0
37	288.0
38	441.0
39	664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.84179543782193	15.15820456217807	12.288447387785135	37.71155261221487
2	13.718278778962853	20.33835969106289	43.9867598381758	21.956601691798454
3	18.536226553880102	23.02317028319235	39.095255608679665	19.345347554247887
4	12.762044869437291	28.24567855829349	31.48216255976462	27.510114012504598
5	11.438028687017287	29.900698786318497	44.354542111070245	14.306730415593968
6	26.737771239426262	45.64178006620081	14.674512688488415	12.945936005884517
7	20.669363736667894	30.047811695476277	19.713129827142332	29.569694740713498
8	20.81647664582567	43.39830820154468	18.60978300845899	17.175432144170653
9	21.662375873482898	16.550202280250094	16.108863552776757	45.67855829349025
10	13.497609415226187	37.072453107760204	27.06877528503126	22.36116219198235
11	26.88488414858404	20.89003310040456	32.622287605737405	19.602795145273998
12	19.014343508642884	23.059948510481796	26.443545421110702	31.48216255976462
13	24.310408238322914	18.168444280985657	34.42442074292019	23.096726737771238
14	18.278778962853988	18.830452372195662	25.81831555719014	37.072453107760204
15	22.140492828245677	23.905847738139023	20.669363736667894	33.28429569694741
16	31.997057741816842	24.420742920191245	21.478484737035675	22.103714600956234
17	20.706141963957336	36.631114380286874	24.420742920191245	18.242000735564545
18	31.886723059948512	23.869069510849577	25.303420375137918	18.940787054063993
19	20.559029054799556	25.34019860242736	22.61860978300846	31.48216255976462
20	19.823464509010666	23.133504965060684	23.059948510481796	33.983082015446854
21	17.80066200809121	25.193085693269584	22.728944464876793	34.27730783376241
22	17.8742184626701	38.24935638102244	23.317396101507907	20.559029054799556
23	17.763883780801766	24.310408238322914	24.86208164766458	33.063626333210735
24	30.673041559396836	25.891872011769035	25.08275101140125	18.35233541743288
25	20.33835969106289	36.00588451636631	25.266642147848472	18.389113644722325
26	31.151158514159615	25.928650239058477	25.045972784111807	17.8742184626701
27	19.897020963589554	37.366678926075764	24.641412283927917	18.094887826406765
28	17.175432144170653	24.567855829349025	26.480323648400145	31.776388378080178
29	25.891872011769035	26.333210739242368	29.900698786318497	17.8742184626701
30	20.559029054799556	32.51195292386907	28.98124310408238	17.94777491724899
31	21.073924236851784	24.82530342037514	31.518940787054063	22.581831555719013
32	21.36815005516734	28.098565649135708	27.105553512320707	23.42773078337624
33	22.434718646561237	27.14233173961015	29.863920559029054	20.559029054799556
34	20.74292019124678	25.119529238690696	29.937477013607943	24.20007355645458
35	22.728944464876793	26.737771239426262	25.450533284295695	25.08275101140125
36	18.205222508275103	24.82530342037514	36.557557925707975	20.411916145641783
37	17.94777491724899	32.622287605737405	28.46634792203016	20.96358955498345
38	17.24898859874954	21.58881941890401	40.27215888194189	20.89003310040456
39	16.95476278043398	19.749908054431774	30.489150422949614	32.80617874218463
40	16.403089371092314	20.228025009194557	40.529606472968005	22.839279146745124
41	17.8742184626701	18.278778962853988	29.863920559029054	33.983082015446854
42	29.422581831555718	20.00735564545789	29.643251195292386	20.926811327694004
43	17.432879735196764	20.632585509378448	40.16182420007355	21.77271055535123
44	15.704303052592866	32.07061419639574	31.886723059948512	20.33835969106289
45	15.777859507171755	20.74292019124678	40.34571533652078	23.133504965060684
46	25.48731151158514	21.147480691430673	30.673041559396836	22.69216623758735
47	14.122839279146746	20.411916145641783	29.974255240897392	35.49098933431409
48	14.196395733725634	19.41890400882677	29.422581831555718	36.96211842589187
49	24.494299374770137	19.970577418168446	31.151158514159615	24.383964692901802
50	12.43104082383229	22.47149687385068	40.639941154836336	24.45752114748069
51	12.509197939661515	21.596762325239148	28.550404709345106	37.34363502575423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.5
12	2.0
13	2.5
14	3.0
15	3.5
16	4.0
17	6.0
18	8.0
19	5.5
20	3.0
21	3.0
22	3.0
23	5.0
24	7.0
25	7.5
26	9.0
27	10.0
28	11.5
29	13.0
30	22.5
31	32.0
32	44.5
33	57.0
34	78.5
35	100.0
36	132.5
37	165.0
38	175.5
39	186.0
40	201.0
41	216.0
42	218.5
43	221.0
44	253.5
45	286.0
46	294.0
47	302.0
48	269.5
49	237.0
50	290.0
51	343.0
52	321.0
53	299.0
54	196.0
55	93.0
56	68.0
57	43.0
58	39.5
59	36.0
60	24.0
61	12.0
62	10.0
63	8.0
64	8.0
65	8.0
66	6.0
67	4.0
68	3.5
69	3.0
70	2.5
71	2.0
72	1.0
73	0.0
74	0.0
75	0.5
76	1.0
77	1.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03677822728944465
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.03677822728944465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2719.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.75983817579993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.34675710046629	85.32548731151158
2	0.8478168715557439	1.471129091577786
3	0.2967359050445104	0.7723427730783377
4	0.0423908435777872	0.1471129091577786
5	0.0423908435777872	0.18389113644722324
6	0.0423908435777872	0.2206693637366679
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.2543450614667232	4.26627436557558
>50	0.1271725307333616	7.613093048915043
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	87	3.199705774181685	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	65	2.3905847738139023	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	55	2.0228025009194557	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	34	1.250459727841118	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	31	1.140125045972784	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	18	0.6620080912100037	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	12	0.4413387274733358	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	11	0.40456050018389117	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	10	0.3677822728944465	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	6	0.2206693637366679	TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	5	0.18389113644722324	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	4	0.1471129091577786	Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.11033468186833395	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG	3	0.11033468186833395	No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT	3	0.11033468186833395	No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA	3	0.11033468186833395	No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC	3	0.11033468186833395	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAA	3	0.11033468186833395	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC	3	0.11033468186833395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.036778227289444645
10	0.0	0.0	0.036778227289444645
11	0.0	0.0	0.036778227289444645
12	0.0	0.0	0.036778227289444645
13	0.0	0.0	0.036778227289444645
14	0.0	0.0	0.036778227289444645
15	0.0	0.0	0.11033468186833395
16	0.0	0.0	0.11033468186833395
17	0.0	0.0	0.11033468186833395
18	0.0	0.0	0.11033468186833395
19	0.0	0.0	0.14711290915777858
20	0.0	0.0	0.14711290915777858
21	0.0	0.0	0.14711290915777858
22	0.0	0.0	0.2206693637366679
23	0.0	0.0	0.25744759102611253
24	0.0	0.0	0.33100404560500185
25	0.0	0.0	0.33100404560500185
26	0.0	0.0	0.33100404560500185
27	0.0	0.0	0.47811695476278043
28	0.0	0.0	0.5884516366311143
29	0.0	0.0	0.9930121368150056
30	0.0	0.0	1.6182420007355645
31	0.0	0.0	3.3835969106289077
32	0.0	0.0	4.6708348657594705
33	0.0	0.0	5.921294593600589
34	0.0	0.0	7.465980139757264
35	0.0	0.0	8.86355277675616
36	0.0	0.0	10.555351232070613
37	0.0	0.0	12.50459727841118
38	0.0	0.0	14.417065097462302
39	0.0	0.0	16.881206325855093
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	25	2.9506173E-5	45.000004	17
ATCTCGT	25	2.9506173E-5	45.000004	37
GCCCACG	25	2.9506173E-5	45.000004	18
TGCCGTC	25	2.9506173E-5	45.000004	45
CCACGAG	25	2.9506173E-5	45.000004	20
TATGCCG	25	2.9506173E-5	45.000004	43
GAGCCCA	25	2.9506173E-5	45.000004	16
CACGAGA	25	2.9506173E-5	45.000004	21
CCCACGA	25	2.9506173E-5	45.000004	19
TCTCGTA	25	2.9506173E-5	45.000004	38
ACGAGAC	25	2.9506173E-5	45.000004	22
ATGCCGT	25	2.9506173E-5	45.000004	44
CGTATGC	25	2.9506173E-5	45.000004	41
CGAGCCC	25	2.9506173E-5	45.000004	15
CCGAGCC	25	2.9506173E-5	45.000004	14
TCGTATG	25	2.9506173E-5	45.000004	40
TCTCCGA	25	2.9506173E-5	45.000004	11
TCCGAGC	25	2.9506173E-5	45.000004	13
CTCGTAT	25	2.9506173E-5	45.000004	39
CTCCGAG	25	2.9506173E-5	45.000004	12
>>END_MODULE