FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_47.34100000004aee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_47.34100000004aee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC873.199705774181685No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC652.3905847738139023No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC552.0228025009194557No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC341.250459727841118No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC311.140125045972784No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC180.6620080912100037No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC120.4413387274733358No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT110.40456050018389117No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC100.3677822728944465RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.2206693637366679TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT50.18389113644722324No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT40.1471129091577786Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11033468186833395No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG30.11033468186833395No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT30.11033468186833395No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA30.11033468186833395No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC30.11033468186833395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGCGAAGCAGTGGTATCAA30.11033468186833395No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC30.11033468186833395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC252.9506173E-545.00000417
ATCTCGT252.9506173E-545.00000437
GCCCACG252.9506173E-545.00000418
TGCCGTC252.9506173E-545.00000445
CCACGAG252.9506173E-545.00000420
TATGCCG252.9506173E-545.00000443
GAGCCCA252.9506173E-545.00000416
CACGAGA252.9506173E-545.00000421
CCCACGA252.9506173E-545.00000419
TCTCGTA252.9506173E-545.00000438
ACGAGAC252.9506173E-545.00000422
ATGCCGT252.9506173E-545.00000444
CGTATGC252.9506173E-545.00000441
CGAGCCC252.9506173E-545.00000415
CCGAGCC252.9506173E-545.00000414
TCGTATG252.9506173E-545.00000440
TCTCCGA252.9506173E-545.00000411
TCCGAGC252.9506173E-545.00000413
CTCGTAT252.9506173E-545.00000439
CTCCGAG252.9506173E-545.00000412
Produced by FastQC (version 0.11.2)