FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_46.34100000004ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_46.34100000004ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638301
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATTA300.005146002429.999483
GTATAGA500.00225448522.5154821
ACGCAAG602.466133E-422.49960911
CAATTGT500.00226374422.4996094
GTATCAA11350.021.2260481
CCCTAAG854.9965583E-621.191041
GTACATT654.2157684E-420.783521
CCACGCA654.2358853E-420.7688679
GGTATCA6200.020.3365631
TATGCCG706.9796306E-419.2853843
TAAGGTG951.3145318E-518.9470395
CATCTCC21150.018.29898645
CTCGTAT800.001709919216.87470639
CTGTGCG800.001709919216.8747069
CCATTAT952.8021488E-416.5903551
TATCAAC13900.016.5105042
ATCTCCG8500.016.41276645
CAACGCA13550.016.106735
ACGCAGA13600.016.0475147
AACGCAG13600.016.0475146
Produced by FastQC (version 0.11.2)