FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_45.34100000004ac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_45.34100000004ac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443362
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG458.6840737E-734.99991243
AAACGGT406.160212E-428.12493117
CGTCGGC406.160212E-428.12493110
CAATACG450.001225384224.9999394
TGCCGTC702.6982092E-522.49994545
CTCGTCC602.4642353E-422.49994319
AAACGGC500.002262466122.49994322
GTATCAA10550.021.5426621
CTTTAGC855.020818E-621.1764183
CTCGTAT754.5945355E-520.99994939
TATTGCG550.00393284120.45449329
TTAGTCC1009.029409E-720.24994916
CTCCGAG3650.019.72597945
TCGTATG807.549771E-519.68745240
GCTTAGG600.00650019518.7520681
CATCTCC16550.018.6253345
GCCCACG851.2023472E-418.52936618
CCCACGA851.2023472E-418.52936619
ATGCCGT851.2023472E-418.52936644
ACTAATC1102.2496843E-618.40904421
Produced by FastQC (version 0.11.2)