FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_44.34100000004ab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_44.34100000004ab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555798
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTA504.8712536E-835.9999317
TCCGATC300.00514504929.99993923
CGGAGGG300.00514504929.9999394
GGTATCA7350.025.1042481
GCGGACA450.001225771724.9999511
CTATAAC651.5219355E-524.230722
GTATCAA14300.023.1314051
ACACCGT500.002263175622.4999566
GATATGC500.002263175622.49995622
TTAGAAC754.5972127E-520.9999583
CCTATAA550.00393201420.4563471
ATCTCCG5500.020.04541445
GTTTAAA706.97313E-419.287411
GGTCCGA1403.074092E-919.287411
TGGACGA706.977354E-419.28567510
CCCTAAA600.00650306118.7516481
AGTAGTG600.0065064418.7499625
AACGCAG16750.018.1342936
CAACGCA16750.017.9999645
TATTGGA750.001109257717.9999642
Produced by FastQC (version 0.11.2)