FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_43.34100000004aab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_43.34100000004aab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725001
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG250.00210705635.9983984
GGTATCA4400.030.1795221
GTATCAA9750.026.3155981
TATAATG500.002264394422.4989992
TATACGT550.003936170620.4536364
CATCTCC20100.020.14974645
TATCAAC13300.019.115692
ACGTAAG600.00650990118.74916632
TAAGACG600.00650990118.7491664
ATCTCCG6650.018.60947845
CAACGCA13750.018.4900885
AACGCAG13800.018.4230946
TCAACGC13850.018.3565854
ATCAACG14050.018.2554173
GTAAGTA2100.018.2134782
ACCACGC1253.8301732E-717.9991998
ACGCAGA14050.017.9351467
GTTCTAA901.8601994E-417.5052591
CGCAGAG14400.017.4992228
CCTTAGC1053.111046E-517.1420962
Produced by FastQC (version 0.11.2)