FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_42.34100000004a9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_42.34100000004a9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC412.0780537252914346RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC261.3177901672579828No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC251.2671059300557528No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC221.1150532184490625No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC130.6588950836289914No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC130.6588950836289914No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC80.40547389761784086No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.3547896604156108No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC70.3547896604156108No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC60.30410542321338063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20273694880892043No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT40.20273694880892043RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC30.15205271160669032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.15205271160669032No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC30.15205271160669032No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT30.15205271160669032No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.15205271160669032No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT20.10136847440446022No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCG20.10136847440446022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG20.10136847440446022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG20.10136847440446022No Hit
GACTACTACCAAGTGGAACCGATCATGCTAGTCCTGTCTCTTATACACATC20.10136847440446022No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.10136847440446022TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA20.10136847440446022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACG20.10136847440446022No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT20.10136847440446022No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA20.10136847440446022No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT20.10136847440446022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10136847440446022No Hit
GTATTGGCATACAGGTCTTTGCGGATATCCACATCACACTTCATGATGGAG20.10136847440446022No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10136847440446022No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT20.10136847440446022No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATACCGTC20.10136847440446022No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAAC20.10136847440446022No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC20.10136847440446022RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA20.10136847440446022No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC250.001633294536.042
ATCTCGT250.001633294536.037
TGCCGTC250.001633294536.045
TATGCCG250.001633294536.043
TCTCGTA250.001633294536.038
ATGCCGT250.001633294536.044
CGTATGC250.001633294536.041
CTCGTAT250.001633294536.039
TCGTATG300.003999065629.99999840
Produced by FastQC (version 0.11.2)