FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_41.34100000004a74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_41.34100000004a74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366279
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA32450.885936676686351No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG27760.757892207852484No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG25970.7090223572740998No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA25930.7079302935740241No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC19080.5209143849360733No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG18130.49497787205927724No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC16690.4556635788565547No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG14340.3915048364771117No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG14010.3824953109514878No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC13720.3745778491259395No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT12730.34754927254906776No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12610.34427308144884095No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA12230.33389847629812247No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12070.32953022149781996No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT12040.32871117372276326No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC11230.30659688379623184No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.2833905301696248No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG10260.2801143390693979No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC9010.24598734844203463No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC8990.24544131659199683No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG8890.24271115734180773No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA8880.24243814141678885No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT8790.23998099809161869No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC8730.23834290254150522No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC8730.23834290254150522No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA8710.23779687069146743No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG8650.236158775141354No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA8620.23533972736629727No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC8490.2317905203410515No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8300.22660321776569228No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT8250.22523813814059773No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA8190.22360004259048433No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT7930.21650162853999272No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA7590.2072190870893499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.2066730552393121No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA7480.20421591191414193No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC7330.20012067303885836No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC6990.19083813158821555No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT6950.1897460678881399No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA6920.1889270201130832No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT6760.1845587653127807No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6680.18237463791262945No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA6590.1799174945874593No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT6160.16817780981164632No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT6070.16572066648647615No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC5700.15561907726077662No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC5590.15261590208556866No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC5370.1466095517351527No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT5280.14415240840998256No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC5260.14360637655994474No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5080.1386920899096044No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT4980.13596193065941536No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC4970.13568891473439645No Hit
GTATCAACGCAGAGTACGGGCAGACACAGAACAAGAGCTGTCTCTTATACA4890.1335047873342452No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT4830.13186669178413177No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC4820.13159367585911286No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4790.13077462808405613No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA4780.13050161215903722No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC4560.1244952618086213No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC4550.1242222458836024No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC4530.12367621403356459No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT4480.12231113440847004No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4410.1204000229333377No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA4370.11930795923326208No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4310.11766986368314863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.1157587522080163No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC4200.11466668850794066No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC4170.11384764073288395No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCCTGTCTCTTATACACATCTC4140.11302859295782725No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4130.11275557703280831No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC4130.11275557703280831No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC4110.1122095451827705No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA4090.1116635133327327No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA4040.11029843370763816No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.10674922668239238No Hit
GTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCAC3880.10593017890733566No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACATC3860.10538414705729786No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA3750.10238097188208987No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA3710.10128890818201426No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.10046986040695755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAAA403.4508594E-739.37320731
AATCTCG403.4508594E-739.37320736
TAAATCT403.4508594E-739.37320734
AGGTCAA301.13687915E-437.5085378
GTAAATA1400.036.967651
ACTAATA552.739398E-936.816512
GGTGGTC250.00210570935.9983637
CGTATGC458.681509E-734.99840541
GATATAA458.681509E-734.99840530
TGCCGTC458.681509E-734.99840545
CGTGGTT606.3919288E-933.7576835
GTGTGTA551.12324415E-732.7302551
CTCGTAT551.1245902E-732.72578439
TGTGTAA551.1245902E-732.7257842
TAAATAC1600.032.3422782
AATACAC1550.031.9340324
GACAGAT501.9774507E-631.49856626
TATGCCG501.9774507E-631.49856643
GTATGCC501.9774507E-631.49856642
TCGTATG501.9774507E-631.49856640
Produced by FastQC (version 0.11.2)