Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 6h_39.34100000004a5b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2301 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 85 | 3.694046066927423 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC | 75 | 3.259452411994785 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC | 48 | 2.0860495436766624 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC | 25 | 1.086484137331595 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 24 | 1.0430247718383312 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC | 22 | 0.9561060408518035 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 12 | 0.5215123859191656 | RNA PCR Primer, Index 27 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC | 7 | 0.3042155584528466 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC | 7 | 0.3042155584528466 | TruSeq Adapter, Index 12 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT | 6 | 0.2607561929595828 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC | 5 | 0.21729682746631898 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT | 4 | 0.1738374619730552 | Illumina PCR Primer Index 8 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT | 4 | 0.1738374619730552 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT | 4 | 0.1738374619730552 | Illumina Paired End PCR Primer 2 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT | 4 | 0.1738374619730552 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT | 3 | 0.1303780964797914 | No Hit |
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGA | 3 | 0.1303780964797914 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATGCCGT | 25 | 2.8056715E-5 | 45.0 | 44 |
AGCCCAC | 30 | 8.240019E-5 | 37.499996 | 17 |
GTATGCC | 30 | 8.240019E-5 | 37.499996 | 42 |
ATCTCGT | 30 | 8.240019E-5 | 37.499996 | 37 |
ATCTCCG | 30 | 8.240019E-5 | 37.499996 | 10 |
CACATCT | 30 | 8.240019E-5 | 37.499996 | 7 |
GCCCACG | 30 | 8.240019E-5 | 37.499996 | 18 |
TGCCGTC | 30 | 8.240019E-5 | 37.499996 | 45 |
CCACGAG | 30 | 8.240019E-5 | 37.499996 | 20 |
TATGCCG | 30 | 8.240019E-5 | 37.499996 | 43 |
GAGCCCA | 30 | 8.240019E-5 | 37.499996 | 16 |
CACGAGA | 30 | 8.240019E-5 | 37.499996 | 21 |
CCCACGA | 30 | 8.240019E-5 | 37.499996 | 19 |
TCTCGTA | 30 | 8.240019E-5 | 37.499996 | 38 |
ACGAGAC | 30 | 8.240019E-5 | 37.499996 | 22 |
CGTATGC | 30 | 8.240019E-5 | 37.499996 | 41 |
CGAGCCC | 30 | 8.240019E-5 | 37.499996 | 15 |
CCGAGCC | 30 | 8.240019E-5 | 37.499996 | 14 |
TCGTATG | 30 | 8.240019E-5 | 37.499996 | 40 |
TCTCCGA | 30 | 8.240019E-5 | 37.499996 | 11 |