FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_39.34100000004a5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_39.34100000004a5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC853.694046066927423No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC753.259452411994785No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC482.0860495436766624No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC251.086484137331595No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC241.0430247718383312No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC220.9561060408518035No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC120.5215123859191656RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.3042155584528466No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC70.3042155584528466TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT60.2607561929595828No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC50.21729682746631898No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT40.1738374619730552Illumina PCR Primer Index 8 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT40.1738374619730552No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT40.1738374619730552Illumina Paired End PCR Primer 2 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT40.1738374619730552No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT30.1303780964797914No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGA30.1303780964797914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT252.8056715E-545.044
AGCCCAC308.240019E-537.49999617
GTATGCC308.240019E-537.49999642
ATCTCGT308.240019E-537.49999637
ATCTCCG308.240019E-537.49999610
CACATCT308.240019E-537.4999967
GCCCACG308.240019E-537.49999618
TGCCGTC308.240019E-537.49999645
CCACGAG308.240019E-537.49999620
TATGCCG308.240019E-537.49999643
GAGCCCA308.240019E-537.49999616
CACGAGA308.240019E-537.49999621
CCCACGA308.240019E-537.49999619
TCTCGTA308.240019E-537.49999638
ACGAGAC308.240019E-537.49999622
CGTATGC308.240019E-537.49999641
CGAGCCC308.240019E-537.49999615
CCGAGCC308.240019E-537.49999614
TCGTATG308.240019E-537.49999640
TCTCCGA308.240019E-537.49999611