FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_38.34100000004a4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_38.34100000004a4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531544
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC9960.18737865538882953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1700.030.44098743
ATGCCGT1700.029.11746644
TGCCGTC1700.029.11746645
CTCGTAT2000.028.12482339
TCGTATG2000.028.12482340
ACGGGTC507.15159E-526.9998316
TCTCGTA2100.026.78554738
TACTTAT1500.025.49984232
GCCCACG2300.025.43462418
CACGAGA2300.025.43462421
CCACGAG2300.025.43462420
TATCTCG1700.025.14690236
CGTATGC2250.024.99984241
TAGTCGC551.3669097E-424.545326
AGTCGCC551.3669097E-424.545327
CTACTTA1600.023.906131
CCCACGA2500.023.39985319
ACGAGAC2450.022.9590422
GTCGCCG602.4651928E-422.4998628
CGCCGTG602.4651928E-422.4998630
Produced by FastQC (version 0.11.2)