FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_37.34100000004a31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_37.34100000004a31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668414
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTA300.005145902729.99985
GTCTTAT406.152883E-428.1332321
CTCGTAT1150.027.39112539
TCGGGAT450.001226065224.99983437
TACTTAT1152.6375346E-1023.47810632
CGTATGC1353.6379788E-1223.33317841
TATGCCG1353.6379788E-1223.33317843
ACTTATC1204.5110937E-1022.49985133
CTACTTA1358.367351E-1121.66652131
TCGTATG1601.8189894E-1221.0936140
CGGCTCA654.2358655E-420.76909323
ACGAGAC1651.8189894E-1220.45440922
TCTCGTA1552.3646862E-1120.32244538
ATGCCGT1552.3646862E-1120.32244544
GTATCAA15800.020.0849911
TGCCGTC1603.8198777E-1119.68736845
GTATTAG1051.4396792E-619.2913571
ATAGGAC1051.4438119E-619.2855853
AGACCGT706.979596E-419.2855856
AGGTCCG2350.019.1488088
Produced by FastQC (version 0.11.2)