FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_36.34100000004a24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_36.34100000004a24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC271.44No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC251.3333333333333335No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC221.1733333333333333No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC201.0666666666666667No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.9066666666666667No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC60.32No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.16No Hit
CTTATACACATCTCCGAGCCACGAGACTGCGACCTATCTCGTATGCCGTCT30.16Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC30.16No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT30.16RNA PCR Primer, Index 44 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC30.16No Hit
ATCAACGCAGAGTACGGGACTAATCCTAGCCCTGTCTCTTATACACATCTC20.10666666666666667No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT20.10666666666666667No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT20.10666666666666667No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT20.10666666666666667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10666666666666667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG20.10666666666666667No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.10666666666666667No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers