FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_34.34100000004a0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_34.34100000004a0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574284
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC11180.1946771980413872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1450.034.137443
ATGCCGT1550.031.93498844
CGCCCCA300.00514333830.00214813
TACATAA300.005145528429.9995372
TCGTATG1750.029.57097240
CTCGTAT1750.028.28527839
TGCCGTC1750.028.28527845
TTCGAAA406.159776E-428.12701215
CCCACGA1850.027.9725419
CTACTTA1500.026.99958231
TACTTAT1500.026.99958232
GTAAATG507.1530216E-526.9995823
CCACGAG1950.026.53805220
ACTTATC1450.026.37890233
GCCCACG2050.025.2457118
ACGAGAC2050.025.24351322
ACTAGGG450.001225917824.9996132
TATCTCG2150.023.02290236
CCTCCGT500.002263444222.49965111
CGAGACC1800.022.49965123
Produced by FastQC (version 0.11.2)