FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_33.341000000049ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_33.341000000049ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70272
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA8171.162625227686703No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA6700.9534380692167577No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5700.8111338797814208No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG5590.7954804189435336No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG5070.7214822404371585No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4090.5820241347905282No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4050.5763319672131147No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3730.5307946265938069No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG3510.49948770491803285No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC2660.37852914389799636No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG2600.3699908925318761No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA2430.3457991803278688No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT2400.34153005464480873No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2390.34010701275045535No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG2260.32160746812386154No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2250.3201844262295082No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC2230.31733834244080145No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA2220.3159153005464481No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC2210.31449225865209474No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA2180.31022313296903464No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC2170.3088000910746812No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.3016848816029144No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2090.29741575591985425No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA2090.29741575591985425No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC2080.2959927140255009No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA2030.28887750455373407No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1970.2803392531876138No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA1940.27607012750455373No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT1920.273224043715847No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA1910.27180100182149364No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1880.26753187613843354No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC1770.25187841530054644No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.2490323315118397No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA1710.24334016393442623No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT1680.23907103825136614No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT1680.23907103825136614No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA1570.22341757741347906No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC1550.22057149362477232No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC1530.21772540983606556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.2163023679417122No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.21487932604735885No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT1380.19637978142076504No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC1240.17645719489981787No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1240.17645719489981787No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT1170.16649590163934425No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT1150.1636498178506375No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC1140.16222677595628415No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT1120.15938069216757741No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1110.15795765027322406No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC1080.15368852459016394No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC1080.15368852459016394No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT1060.1508424408014572No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC1050.14941939890710382No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1030.14657331511839708No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA1030.14657331511839708No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT1020.14515027322404372No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA1020.14515027322404372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.14372723132969034No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA990.1408811475409836No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTATA970.13803506375227687No Hit
TTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTA970.13803506375227687No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC960.1366120218579235No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC960.1366120218579235No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA950.13518897996357013No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC950.13518897996357013No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC900.12807377049180327No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC860.12238160291438979No Hit
TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA840.11953551912568307No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA840.11953551912568307No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA830.11811247723132969No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT830.11811247723132969No Hit
GATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG810.11526639344262295No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC800.11384335154826959No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC800.11384335154826959No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCCTGTCTCTTATACACATCTC800.11384335154826959No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC800.11384335154826959No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTAT790.11242030965391621No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATAC770.10957422586520948No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC770.10957422586520948No Hit
GTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGAG760.1081511839708561No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCACTGTCTCTTATACACATCTC750.10672814207650275No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.10530510018214936No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTCC740.10530510018214936No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC740.10530510018214936No Hit
ATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA740.10530510018214936No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA730.10388205828779598No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA730.10388205828779598No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC720.10245901639344263No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTA720.10245901639344263No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT710.10103597449908924No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATA710.10103597449908924No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC710.10103597449908924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC406.65932E-945.00000442
TGCCGTC406.65932E-945.00000445
CCACGAG406.65932E-945.00000420
TATGCCG406.65932E-945.00000443
TATGTAA206.988102E-445.0000042
TCTCGTA406.65932E-945.00000438
ACGAGAC406.65932E-945.00000422
ATGCCGT406.65932E-945.00000444
CTCGTAT406.65932E-945.00000439
TTATCTC351.1902921E-745.035
TCTACTT351.1902921E-745.030
CCTCTAC351.1902921E-745.028
ACTTATC351.1902921E-745.033
ACCTCTA351.1902921E-745.027
CGAGACC351.1902921E-745.023
TACTTAT351.1902921E-745.032
CTACTTA351.1902921E-745.031
GAGACCT351.1902921E-745.024
CACGAGA451.884473E-840.021
CCCACGA451.884473E-840.019