##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_33.341000000049ef.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70272 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.282786885245905 38.0 38.0 38.0 32.0 38.0 2 36.32100979052823 38.0 38.0 38.0 32.0 38.0 3 36.27653973132969 38.0 38.0 38.0 32.0 38.0 4 36.31332536429873 38.0 38.0 38.0 32.0 38.0 5 36.25890824225865 38.0 38.0 38.0 32.0 38.0 6 38.111495332422585 40.0 38.0 40.0 38.0 40.0 7 38.04197973588342 40.0 38.0 40.0 32.0 40.0 8 37.92372495446266 40.0 38.0 40.0 32.0 40.0 9 37.95420651183971 40.0 38.0 40.0 32.0 40.0 10 37.81331113387978 40.0 38.0 40.0 32.0 40.0 11 37.72740209471767 40.0 38.0 40.0 32.0 40.0 12 37.724556010928964 40.0 38.0 40.0 32.0 40.0 13 37.70814833788707 40.0 38.0 40.0 32.0 40.0 14 37.717611566484514 40.0 38.0 40.0 32.0 40.0 15 37.778560450819676 40.0 38.0 40.0 32.0 40.0 16 37.73912795992714 40.0 38.0 40.0 32.0 40.0 17 37.72274874772313 40.0 38.0 40.0 32.0 40.0 18 37.66330828779599 40.0 38.0 40.0 32.0 40.0 19 37.61609175774135 40.0 38.0 40.0 32.0 40.0 20 37.57476662112933 40.0 38.0 40.0 32.0 40.0 21 37.552809084699454 40.0 38.0 40.0 32.0 40.0 22 37.5617173269581 40.0 38.0 40.0 32.0 40.0 23 37.59246926229508 40.0 38.0 40.0 32.0 40.0 24 37.62595343806922 40.0 38.0 40.0 32.0 40.0 25 37.59460382513661 40.0 38.0 40.0 32.0 40.0 26 37.54657616120219 40.0 38.0 40.0 32.0 40.0 27 37.54733037340619 40.0 38.0 40.0 32.0 40.0 28 37.50348645264116 40.0 38.0 40.0 32.0 40.0 29 37.438809198542806 40.0 38.0 40.0 32.0 40.0 30 37.39376992258652 40.0 38.0 40.0 32.0 40.0 31 37.36304644808743 40.0 38.0 40.0 32.0 40.0 32 37.3110627276867 40.0 38.0 40.0 32.0 40.0 33 37.15552424863388 40.0 38.0 40.0 32.0 40.0 34 37.14443875227687 40.0 38.0 40.0 32.0 40.0 35 37.08432946265938 40.0 38.0 40.0 32.0 40.0 36 36.99057946265938 40.0 38.0 40.0 32.0 40.0 37 36.98058970856102 40.0 38.0 40.0 32.0 40.0 38 37.01240892531876 40.0 38.0 40.0 32.0 40.0 39 37.04012978142077 40.0 38.0 40.0 32.0 40.0 40 37.01250853825137 40.0 38.0 40.0 32.0 40.0 41 36.9656762295082 40.0 38.0 40.0 32.0 40.0 42 36.91068989071038 40.0 38.0 40.0 32.0 40.0 43 36.82344319216758 40.0 38.0 40.0 32.0 40.0 44 36.76884107468124 40.0 38.0 40.0 32.0 40.0 45 36.73387693533698 38.0 38.0 40.0 32.0 40.0 46 36.62546960382514 38.0 38.0 40.0 32.0 40.0 47 36.52722279143898 38.0 38.0 40.0 27.0 40.0 48 36.47649134790528 38.0 38.0 40.0 27.0 40.0 49 36.36664674408014 38.0 38.0 40.0 27.0 40.0 50 36.25651753187614 38.0 38.0 40.0 27.0 40.0 51 35.439606102003644 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.04452928160094416 2201 2 -0.27671280440223 2201 3 0.424087090233364 2201 4 0.14613223109911644 2201 5 0.2457063197061018 2201 6 0.35729545305858323 2201 7 0.1549543649798366 2201 8 0.0687630227666034 2201 9 0.30982345414075496 2201 10 -0.3358638086251702 2201 11 0.38119041543819066 2201 12 0.4481199866923333 2201 13 0.318643725830853 2201 14 0.30028961611413507 2201 15 0.27749525779826456 2201 16 -0.4935323111275238 2201 17 0.11325041256483104 2201 18 0.11431361285681163 2201 19 0.267296343958769 2201 20 0.5356217516714494 2201 21 0.3989839260380279 2201 22 0.44320610881453604 2201 23 0.4646170133657108 2201 24 -0.3714051676146042 2201 25 -0.06798774455191392 2201 26 -0.11704031158279804 2201 27 -0.25192093463517296 2201 28 0.2677035748042229 2201 29 0.7613842171969765 2201 30 0.09381511605593573 2201 31 0.6798535592081194 2201 32 0.30315426349379493 2201 33 0.07353962473676035 2201 34 0.4442430391612149 2201 35 0.3218517379822359 2201 36 -0.050419596830202806 2201 37 -0.2704010460699493 2201 38 -0.797143707661732 2201 39 -0.24633216348488673 2201 40 0.032444282497579024 2201 41 0.3570050175862889 2201 42 0.26833730887739193 2201 43 0.4752439454842303 2201 44 0.5617304852080451 2201 45 0.8680010607378676 2201 46 -0.36936898178920785 2201 47 -0.17458030118508816 2201 48 -0.6314950390270653 2201 49 -0.13153980983158675 2201 50 -2.009680960980134 2201 51 -0.1935537354017427 1101 1 0.09086311688415805 1101 2 0.6203102466808232 1101 3 -0.32211932700281665 1101 4 -0.019279560861960476 1101 5 0.7279257104161729 1101 6 0.2828138128408142 1101 7 0.305075991297457 1101 8 0.4737207431419037 1101 9 0.7150128436924703 1101 10 0.46408985753954823 1101 11 0.7084231271259043 1101 12 0.4671168591584518 1101 13 0.8266942297113076 1101 14 0.7212325096621512 1101 15 0.1211185637174097 1101 16 -0.1024747413371827 1101 17 -0.0424312739867716 1101 18 -0.3713808154994922 1101 19 -0.08611498466395773 1101 20 0.9611980287477806 1101 21 1.1572371404478545 1101 22 0.4573379285759174 1101 23 -0.30093378010121796 1101 24 0.5061576726495005 1101 25 0.00660973025406264 1101 26 0.9752798552190072 1101 27 0.6340978305681162 1101 28 0.7989209963262951 1101 29 1.0999687185290767 1101 30 0.9799497158474324 1101 31 0.7661503274231052 1101 32 0.8773463172410487 1101 33 0.43088932935856405 1101 34 0.5731669358367668 1101 35 -0.3590239715046195 1101 36 0.023598705034736156 1101 37 0.14451844889124743 1101 38 0.2517384885620615 1101 39 0.703743128997445 1101 40 -0.13435752452199523 1101 41 -0.16193393758572228 1101 42 0.2432012032362536 1101 43 0.10526827074775014 1101 44 0.2199026154061201 1101 45 0.1600200576103319 1101 46 0.45375160201712106 1101 47 0.4433972269047999 1101 48 0.8726170888543194 1101 49 1.177970209860291 1101 50 0.43859889538497043 1101 51 0.6427719914603003 2202 1 -0.281428067630884 2202 2 0.0078549552859144 2202 3 0.09572203634664334 2202 4 -0.22683268520448507 2202 5 0.5543511259432066 2202 6 0.665940259295688 2202 7 0.45477092081565473 2202 8 0.7849978312967565 2202 9 -0.19052050366708784 2202 10 -0.5700990757657323 2202 11 -0.24550071045036503 2202 12 0.5355999224868739 2202 13 -0.4651214656389939 2202 14 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0.40698346464674273 2202 50 1.1859163790794796 2202 51 0.14077325382091033 1102 1 0.4381027353445077 1102 2 0.3099720638115713 1102 3 -0.027871326232030924 1102 4 -0.08845770904579808 1102 5 0.5680837217852783 1102 6 0.13761678037140967 1102 7 -0.5108022243302486 1102 8 -0.2173862999258276 1102 9 -0.14875052464744698 1102 10 0.7117052347435191 1102 11 0.6257562035301802 1102 12 0.10369071020383558 1102 13 -0.05161774639428529 1102 14 0.4065586489819282 1102 15 0.5893717673015715 1102 16 0.5564519375336161 1102 17 0.08542304723877692 1102 18 -0.042254695449393864 1102 19 0.7590531432867138 1102 20 -0.15239672989948616 1102 21 0.3708415339347013 1102 22 0.1402553580007151 1102 23 0.38207999771938006 1102 24 0.13820314909231257 1102 25 0.21501131036774979 1102 26 0.3586501221040663 1102 27 0.09502855607154004 1102 28 0.31852789487142985 1102 29 0.2674222304281031 1102 30 0.3171691011446711 1102 31 -0.6791719579608397 1102 32 -0.09808484183015764 1102 33 0.3234608680424458 1102 34 -0.22035845196553083 1102 35 0.498476751108349 1102 36 0.59822053758122 1102 37 -0.6810909547121824 1102 38 0.10162768790689114 1102 39 0.33174197281670104 1102 40 -0.27040145939047733 1102 41 0.3732814010788914 1102 42 0.060524644800949545 1102 43 -0.3681877462095642 1102 44 -0.17522215576643418 1102 45 0.1341986875361414 1102 46 0.454426040787709 1102 47 0.22844395587676303 1102 48 0.5369392778898714 1102 49 0.7147271228576884 1102 50 0.7037496806243979 1102 51 0.2786714037350819 2203 1 0.13107950965745374 2203 2 -0.09337761695090308 2203 3 0.27979962777925493 2203 4 -0.08225802365669921 2203 5 0.4106857389963494 2203 6 0.11633427828942189 2203 7 0.6682483910116588 2203 8 0.46402389716865144 2203 9 0.794526980673183 2203 10 -0.2725642202393601 2203 11 0.4120058565328648 2203 12 0.5744966693044162 2203 13 0.8525184616190344 2203 14 0.005707348898653208 2203 15 0.24033873315703858 2203 16 0.38665458396648233 2203 17 0.8295703591662331 2203 18 0.4987835205217337 2203 19 0.6146097269824651 2203 20 0.3071202498358687 2203 21 0.3923091012521809 2203 22 0.48336630460939034 2203 23 0.2776106722821652 2203 24 0.5561729834589286 2203 25 0.32660564737154374 2203 26 0.6545694336613863 2203 27 0.6333833261279835 2203 28 0.48841778105631306 2203 29 0.1253846621852972 2203 30 0.5089892177357669 2203 31 0.4724890025277304 2203 32 0.07587647953562282 2203 33 0.2368503378305462 2203 34 -0.147257683944801 2203 35 0.0340555426323661 2203 36 -0.09725461741091124 2203 37 0.6540621867701901 2203 38 0.4118895552151187 2203 39 0.5480832331109511 2203 40 1.1386852436723487 2203 41 0.6787315722642333 2203 42 1.0885620279785186 2203 43 0.5264176021757407 2203 44 0.3712754846518109 2203 45 -0.1024072160448668 2203 46 -0.708338833830588 2203 47 0.0077536625483674015 2203 48 0.3719536204456233 2203 49 -0.002493197305163619 2203 50 -0.5025055243353478 2203 51 -0.09765875264503876 1103 1 -0.33706919200516694 1103 2 -0.41917623454850883 1103 3 -0.3076279829063395 1103 4 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25 0.2893368261497997 2204 26 0.2169643523985485 2204 27 0.5169999896811319 2204 28 0.9304129239668271 2204 29 0.6709665788675281 2204 30 -0.1948217293966792 2204 31 0.2898811190978776 2204 32 0.49872348121692767 2204 33 0.15876328384212712 2204 34 0.20320000333336452 2204 35 -0.032822843319436856 2204 36 0.3305428793198928 2204 37 0.07653556618360113 2204 38 0.5644208460800542 2204 39 0.8723873616370099 2204 40 0.8307270893663556 2204 41 0.81015320499354 2204 42 -0.13785306636110306 2204 43 0.39966624779501814 2204 44 0.6788600166899172 2204 45 0.22712762423161337 2204 46 0.4836096062964401 2204 47 0.16981232124442158 2204 48 0.2921852662517139 2204 49 0.41372200912113755 2204 50 -0.012966947836190457 2204 51 0.37142400468729875 2205 1 -0.24323048771396572 2205 2 0.10411646874085534 2205 3 0.2546528653153999 2205 4 -0.12054769333163051 2205 5 -0.33548091306708727 2205 6 0.15663919373672286 2205 7 0.04597554425795636 2205 8 -0.16271753803681577 2205 9 0.12318728033146442 2205 10 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0.270780477921285 1212 49 0.2515212797727955 1212 50 -0.1646238370069497 1212 51 -0.5278942131658795 1213 1 -0.44091706007826303 1213 2 0.15087279234199968 1213 3 -0.10594918332232339 1213 4 -0.2177534629644029 1213 5 -0.723328449824109 1213 6 -0.8794558519047015 1213 7 -0.5602539867851775 1213 8 -0.41482540508441446 1213 9 -0.5154441739225959 1213 10 0.011212188897616215 1213 11 0.12152483992726815 1213 12 -0.3333539582780247 1213 13 -0.25943289099654265 1213 14 -0.3654628389862822 1213 15 -0.3095170398218343 1213 16 -0.29526636403382867 1213 17 -0.07360263529857036 1213 18 -0.08280219710514558 1213 19 -0.46171365338706494 1213 20 -0.9794627391716944 1213 21 -0.043022620896408625 1213 22 -0.3756030568936879 1213 23 -0.8895367511215895 1213 24 -0.9199321073651134 1213 25 0.060916086289452664 1213 26 -0.2840756119455321 1213 27 -0.1693764774114257 1213 28 -0.04522219711473241 1213 29 -0.43741256241905546 1213 30 -0.3881808142280576 1213 31 -0.03659340177568993 1213 32 -0.6117355887578171 1213 33 -0.25009126971603735 1213 34 0.5202405619427779 1213 35 0.020958169902968393 1213 36 -0.8310972930170593 1213 37 -0.8005257917127011 1213 38 -0.08043133041675077 1213 39 -0.4727322342625584 1213 40 0.23831882907813196 1213 41 -0.8957597351342272 1213 42 -0.05317025548605159 1213 43 0.45976133024933574 1213 44 -0.7158064518882981 1213 45 -0.3776859250181275 1213 46 -0.22958308423988427 1213 47 -0.7692011982920519 1213 48 -0.542757535709363 1213 49 -0.17400641161871988 1213 50 0.13240521083265833 1213 51 -0.5294822499554002 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 3.0 17 2.0 18 1.0 19 2.0 20 9.0 21 26.0 22 33.0 23 95.0 24 155.0 25 279.0 26 475.0 27 596.0 28 858.0 29 1114.0 30 1442.0 31 1745.0 32 1977.0 33 2524.0 34 2845.0 35 3507.0 36 4521.0 37 6513.0 38 11014.0 39 30534.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.525365825883966 13.041621590844388 24.826339463645162 15.606673119626487 2 16.317062757933684 23.567667567952185 47.20222000853849 12.913049665575638 3 11.859631147540984 31.373804644808743 42.31699681238615 14.449567395264118 4 10.27436247723133 23.333617941712205 40.23935564663024 26.15266393442623 5 9.38780737704918 30.00341530054645 33.79012978142077 26.81864754098361 6 22.00591985428051 36.99624316939891 16.235484972677597 24.762352003642988 7 16.740664845173043 41.33794398907104 20.180157103825135 21.741234061930783 8 24.65277777777778 28.287226775956288 16.922814207650273 30.13718123861566 9 30.03472222222222 15.62073087431694 16.72643442622951 37.61811247723133 10 20.043545081967213 21.505009107468123 24.73673724954463 33.71470856102003 11 27.90624866587924 26.29107313116364 18.179618903957536 27.623059298999586 12 31.403688524590162 28.166268214936245 21.11936475409836 19.31067850637523 13 31.615721766848814 25.804018670309652 25.879439890710383 16.700819672131146 14 25.132342896174865 27.35228825136612 27.91581284153005 19.59955601092896 15 24.032331511839708 23.747723132969035 29.688923041894355 22.5310223132969 16 15.647991347782158 28.9109305403367 27.86782598796089 27.57325212392025 17 22.11153961093481 24.855203426733645 20.708400335842665 32.32485662648888 18 31.462928703571936 18.344955172904513 22.27550875195674 27.916607371566815 19 38.443476775956285 18.01997950819672 21.882115209471767 21.65442850637523 20 35.795195810564664 14.441029143897996 21.02686703096539 28.73690801457195 21 31.425034153005466 18.1366689435337 24.405168488160292 26.033128415300546 22 29.047131147540984 17.049464936247723 28.84363615664845 25.05976775956284 23 28.22603597449909 17.95167349726776 32.294512750455375 21.52777777777778 24 29.410006830601095 19.581056466302368 29.795651183970858 21.213285519125684 25 22.932320127504553 24.139059653916213 31.915983606557376 21.012636612021858 26 19.660746812386158 26.68915072859745 34.04627732240437 19.60382513661202 27 15.121243169398907 27.94854280510018 34.06193078324226 22.868283242258652 28 14.606102003642988 27.634050546448087 28.13353825136612 29.626309198542806 29 16.831739526411656 28.633025956284154 26.72045765027322 27.814776867030965 30 21.199960153125843 22.867836457043648 28.250010673269866 27.682192716560643 31 22.717440801457194 22.63917349726776 28.364071038251364 26.27931466302368 32 23.537112932604735 20.120389344262296 31.09204234972678 25.250455373406194 33 21.14924863387978 18.034209927140253 31.557377049180328 29.259164389799636 34 20.488957194899818 21.018328779599273 34.78198998178507 23.710724043715846 35 18.456853369763206 23.67372495446266 34.7563752276867 23.11304644808743 36 18.14663023679417 23.48446038251366 36.2306466302368 22.13826275045537 37 12.9852572859745 28.435223132969035 38.4093237704918 20.17019581056466 38 14.224726775956285 26.57388433515483 36.77709471766849 22.424294171220403 39 11.176571038251366 30.454519581056466 36.79844034608379 21.57046903460838 40 14.189150728597449 26.478540528233154 37.13712431693989 22.19518442622951 41 14.944785974499089 27.31671220400729 34.064776867030965 23.67372495446266 42 13.803506375227686 28.55048952641166 33.56386612021858 24.082137978142075 43 11.787056010928962 27.732240437158467 30.908469945355193 29.572233606557376 44 9.532957650273225 28.383993624772312 33.263604280510016 28.819444444444443 45 8.393101092896176 26.413080601092897 35.09363615664845 30.100182149362475 46 9.100352914389799 23.747723132969035 35.31278460837887 31.839139344262296 47 7.512238160291439 23.033356102003644 38.88319672131148 30.571209016393443 48 7.4723929872495445 24.16752049180328 35.71550546448087 32.64458105646631 49 9.451844262295083 23.629610655737704 34.64110883424408 32.27743624772314 50 10.873463114754097 26.17970173041894 30.418943533697636 32.52789162112932 51 13.723816029143897 27.074795081967213 26.585268670309652 32.61612021857923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 2.0 3 2.0 4 2.0 5 5.5 6 9.0 7 13.5 8 18.0 9 20.5 10 23.0 11 55.0 12 87.0 13 196.5 14 306.0 15 418.5 16 531.0 17 588.5 18 646.0 19 593.5 20 541.0 21 533.5 22 526.0 23 608.0 24 690.0 25 710.5 26 562.5 27 394.0 28 298.5 29 203.0 30 201.5 31 200.0 32 335.0 33 470.0 34 517.5 35 565.0 36 652.5 37 740.0 38 920.5 39 1101.0 40 1968.0 41 2835.0 42 4961.0 43 7087.0 44 8852.5 45 10618.0 46 12494.0 47 14370.0 48 15513.5 49 16657.0 50 11539.5 51 6422.0 52 4499.0 53 2576.0 54 1766.5 55 957.0 56 635.0 57 313.0 58 253.0 59 193.0 60 159.0 61 125.0 62 108.0 63 91.0 64 88.5 65 86.0 66 66.5 67 47.0 68 43.0 69 39.0 70 26.0 71 13.0 72 14.5 73 16.0 74 14.5 75 13.0 76 13.0 77 10.5 78 8.0 79 6.5 80 5.0 81 2.5 82 0.0 83 1.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028460837887067398 2 0.0028460837887067394 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0014230418943533697 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0014230418943533697 17 0.0014230418943533697 18 0.0028460837887067394 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.00426912568306011 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 70272.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.04891786726003 #Duplication Level Percentage of deduplicated Percentage of total 1 82.59742041278103 37.2092440822292 2 8.687530437067153 7.827276902575196 3 2.7115954491334637 3.664633220317484 4 1.4500912144215614 2.613001600740491 5 0.8429003197251597 1.8985873636792965 6 0.4549965388435443 1.229826102495025 7 0.4687440875664351 1.4781489731080855 8 0.33730979694554075 1.2156353070737458 9 0.2797517115037252 1.1342260687281065 >10 1.7484533954931891 15.796600517040735 >50 0.23752281273781145 7.408776540851468 >100 0.16784901138681788 14.068514417020724 >500 0.015834812394577447 4.455528904140414 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA 817 1.162625227686703 No Hit GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA 670 0.9534380692167577 No Hit GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG 570 0.8111338797814208 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG 559 0.7954804189435336 No Hit GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG 507 0.7214822404371585 No Hit TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC 409 0.5820241347905282 No Hit TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG 405 0.5763319672131147 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC 373 0.5307946265938069 No Hit TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG 351 0.49948770491803285 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC 266 0.37852914389799636 No Hit GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG 260 0.3699908925318761 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA 243 0.3457991803278688 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT 240 0.34153005464480873 No Hit TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA 239 0.34010701275045535 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG 226 0.32160746812386154 No Hit ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG 225 0.3201844262295082 No Hit ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC 223 0.31733834244080145 No Hit TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA 222 0.3159153005464481 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC 221 0.31449225865209474 No Hit TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA 218 0.31022313296903464 No Hit TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC 217 0.3088000910746812 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 212 0.3016848816029144 No Hit GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC 209 0.29741575591985425 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA 209 0.29741575591985425 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC 208 0.2959927140255009 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA 203 0.28887750455373407 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 197 0.2803392531876138 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA 194 0.27607012750455373 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT 192 0.273224043715847 No Hit TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA 191 0.27180100182149364 No Hit CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT 188 0.26753187613843354 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC 177 0.25187841530054644 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.2490323315118397 No Hit TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA 171 0.24334016393442623 No Hit TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT 168 0.23907103825136614 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT 168 0.23907103825136614 No Hit ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA 157 0.22341757741347906 No Hit GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC 155 0.22057149362477232 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC 153 0.21772540983606556 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.2163023679417122 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.21487932604735885 No Hit GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT 138 0.19637978142076504 No Hit CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC 124 0.17645719489981787 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 124 0.17645719489981787 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT 117 0.16649590163934425 No Hit TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT 115 0.1636498178506375 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC 114 0.16222677595628415 No Hit TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT 112 0.15938069216757741 No Hit GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC 111 0.15795765027322406 No Hit TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC 108 0.15368852459016394 No Hit GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC 108 0.15368852459016394 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT 106 0.1508424408014572 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC 105 0.14941939890710382 No Hit GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT 103 0.14657331511839708 No Hit TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA 103 0.14657331511839708 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT 102 0.14515027322404372 No Hit TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA 102 0.14515027322404372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.14372723132969034 No Hit TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA 99 0.1408811475409836 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTATA 97 0.13803506375227687 No Hit TTTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTA 97 0.13803506375227687 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC 96 0.1366120218579235 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC 96 0.1366120218579235 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 95 0.13518897996357013 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC 95 0.13518897996357013 No Hit TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC 90 0.12807377049180327 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATC 86 0.12238160291438979 No Hit TTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA 84 0.11953551912568307 No Hit GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA 84 0.11953551912568307 No Hit GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA 83 0.11811247723132969 No Hit CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT 83 0.11811247723132969 No Hit GATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG 81 0.11526639344262295 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 80 0.11384335154826959 No Hit GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC 80 0.11384335154826959 No Hit GTATCAACGCAGAGTACGGGGCAGTGGTATCCTGTCTCTTATACACATCTC 80 0.11384335154826959 No Hit GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC 80 0.11384335154826959 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTAT 79 0.11242030965391621 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATAC 77 0.10957422586520948 No Hit CGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC 77 0.10957422586520948 No Hit GTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGAG 76 0.1081511839708561 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCACTGTCTCTTATACACATCTC 75 0.10672814207650275 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.10530510018214936 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACTGTCTCTTATACACATCTCC 74 0.10530510018214936 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC 74 0.10530510018214936 No Hit ATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA 74 0.10530510018214936 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA 73 0.10388205828779598 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA 73 0.10388205828779598 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC 72 0.10245901639344263 No Hit TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTA 72 0.10245901639344263 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT 71 0.10103597449908924 No Hit TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATA 71 0.10103597449908924 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC 71 0.10103597449908924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0028460837887067394 3 0.0 0.0 0.00426912568306011 4 0.0 0.0 0.00426912568306011 5 0.0 0.0 0.00426912568306011 6 0.0 0.0 0.00426912568306011 7 0.0 0.0 0.00426912568306011 8 0.0 0.0 0.00426912568306011 9 0.0 0.0 0.00426912568306011 10 0.0 0.0 0.005692167577413479 11 0.0 0.0 0.00853825136612022 12 0.0 0.0 0.00853825136612022 13 0.0 0.0 0.012807377049180328 14 0.0 0.0 0.01707650273224044 15 0.0 0.0 0.01707650273224044 16 0.0 0.0 0.025614754098360656 17 0.0 0.0 0.027037795992714025 18 0.0 0.0 0.03415300546448088 19 0.0 0.0 0.0469603825136612 20 0.0 0.0 0.06403688524590163 21 0.0 0.0 0.19922586520947178 22 0.0 0.0 0.3856443533697632 23 0.0 0.0 0.8125569216757741 24 0.0 0.0 1.3831967213114753 25 0.0 0.0 1.6635359744990892 26 0.0 0.0 2.190061475409836 27 0.0 0.0 2.85889116575592 28 0.0 0.0 4.972108378870674 29 0.0 0.0 9.774874772313296 30 0.0 0.0 15.39731329690346 31 0.0 0.0 20.251309198542806 32 0.0 0.0 24.17321265938069 33 0.0 0.0 28.23030510018215 34 0.0 0.0 32.00563524590164 35 0.0 0.0 34.45184426229508 36 0.0 0.0 36.419911202185794 37 0.0 0.0 39.12795992714025 38 0.0 0.0 42.52191484517304 39 0.0 0.0 47.869706284153004 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 40 6.65932E-9 45.000004 42 TGCCGTC 40 6.65932E-9 45.000004 45 CCACGAG 40 6.65932E-9 45.000004 20 TATGCCG 40 6.65932E-9 45.000004 43 TATGTAA 20 6.988102E-4 45.000004 2 TCTCGTA 40 6.65932E-9 45.000004 38 ACGAGAC 40 6.65932E-9 45.000004 22 ATGCCGT 40 6.65932E-9 45.000004 44 CTCGTAT 40 6.65932E-9 45.000004 39 TTATCTC 35 1.1902921E-7 45.0 35 TCTACTT 35 1.1902921E-7 45.0 30 CCTCTAC 35 1.1902921E-7 45.0 28 ACTTATC 35 1.1902921E-7 45.0 33 ACCTCTA 35 1.1902921E-7 45.0 27 CGAGACC 35 1.1902921E-7 45.0 23 TACTTAT 35 1.1902921E-7 45.0 32 CTACTTA 35 1.1902921E-7 45.0 31 GAGACCT 35 1.1902921E-7 45.0 24 CACGAGA 45 1.884473E-8 40.0 21 CCCACGA 45 1.884473E-8 40.0 19 >>END_MODULE