H23NVBCXX l02n01 6h_32.341000000049d2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 6h_32.341000000049d2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1175
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	141	12.0	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	99	8.425531914893616	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	68	5.787234042553192	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	41	3.4893617021276593	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	39	3.3191489361702122	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	36	3.0638297872340425	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	34	2.893617021276596	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	17	1.446808510638298	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	9	0.7659574468085106	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTAAATCTCGTATGCCGTCT	7	0.5957446808510638	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	7	0.5957446808510638	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	7	0.5957446808510638	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	6	0.5106382978723404	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	4	0.3404255319148936	RNA PCR Primer, Index 13 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	4	0.3404255319148936	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	4	0.3404255319148936	Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.3404255319148936	No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	3	0.2553191489361702	Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGCC	3	0.2553191489361702	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT	3	0.2553191489361702	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	3	0.2553191489361702	No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC	3	0.2553191489361702	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT	2	0.1702127659574468	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTAAATCTCGTATGCCGTCT	2	0.1702127659574468	No Hit
CTTATACACATCTCCGAGCCAACGAGACCTGATTAAATCTCGTATGCCGTC	2	0.1702127659574468	No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT	2	0.1702127659574468	RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	2	0.1702127659574468	No Hit
CTTATACAATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT	2	0.1702127659574468	No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2	0.1702127659574468	No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTC	2	0.1702127659574468	No Hit
CTTATACTCATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	2	0.1702127659574468	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCTT	20	4.485316E-4	45.000004	27
GTATCTC	20	4.485316E-4	45.000004	35
CTTAGTA	20	4.485316E-4	45.000004	31
AGTATCT	20	4.485316E-4	45.000004	34
TAGTATC	20	4.485316E-4	45.000004	33
AGACGTC	20	4.485316E-4	45.000004	25
GACGTCT	20	4.485316E-4	45.000004	26
GTCTTAG	20	4.485316E-4	45.000004	29
TTAGTAT	20	4.485316E-4	45.000004	32
CGTCTTA	20	4.485316E-4	45.000004	28
TCTTAGT	20	4.485316E-4	45.000004	30
GAGACGT	20	4.485316E-4	45.000004	24
CTATCTC	20	4.485316E-4	45.000004	35
CCTATCT	15	0.009799245	44.999996	34
ACCTATC	15	0.009799245	44.999996	33
TATACAC	65	0.0	41.538464	3
TTATACA	65	0.0	41.538464	2
TACACAT	65	0.0	41.538464	5
ACACATC	65	0.0	41.538464	6
CTTATAC	65	0.0	41.538464	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content