H23NVBCXX l02n01 6h_31.341000000049c5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 6h_31.341000000049c5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3132
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	123	3.9272030651340994	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	101	3.2247765006385696	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	54	1.7241379310344827	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	36	1.1494252873563218	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	30	0.9578544061302682	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	24	0.7662835249042145	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	20	0.6385696040868455	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	17	0.5427841634738186	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	13	0.41507024265644954	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	11	0.351213282247765	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	10	0.31928480204342274	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	8	0.2554278416347382	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	7	0.2234993614303959	Illumina PCR Primer Index 8 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	4	0.1277139208173691	RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	4	0.1277139208173691	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT	4	0.1277139208173691	No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	4	0.1277139208173691	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCT	20	5.999368E-4	45.000004	27
GCTGGAA	20	5.999368E-4	45.000004	31
TGCTGGA	20	5.999368E-4	45.000004	30
CTGGAAT	20	5.999368E-4	45.000004	32
CTTGCTG	20	5.999368E-4	45.000004	28
TGGAATC	20	5.999368E-4	45.000004	33
AGACTTG	20	5.999368E-4	45.000004	25
CGAGACT	20	5.999368E-4	45.000004	23
GGAATCT	20	5.999368E-4	45.000004	34
GACTTGC	20	5.999368E-4	45.000004	26
GAGACTT	20	5.999368E-4	45.000004	24
TTGCTGG	20	5.999368E-4	45.000004	29
AATCTCG	25	3.061651E-5	45.0	36
GAATCTC	25	3.061651E-5	45.0	35
TGCCGTC	60	1.8189894E-12	41.249996	45
GTATGCC	65	3.6379788E-12	38.076927	42
ATCTCGT	65	3.6379788E-12	38.076927	37
TATGCCG	65	3.6379788E-12	38.076927	43
GAGCCCA	65	3.6379788E-12	38.076927	16
TCTCGTA	65	3.6379788E-12	38.076927	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content