FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_30.341000000049b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_30.341000000049b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652589
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1450.032.58487743
CGAGCCC1600.029.53230715
CGTATGC1650.028.63519541
ACGCAAT406.161975E-428.12600515
GGTATCA4550.027.2029421
GACCTAT1004.5474735E-1126.99889626
ACGAGAC1900.026.05156722
CTCGTAT1950.025.38357739
GTATCAA9650.024.4864941
TCGTATG1950.024.22977840
AATCTCG1400.024.1061636
CCACGAG2100.023.57046720
CCGAGCC2050.023.04960414
TGCCGTC2050.023.04783845
GGGGGCG500.00226309922.50080518
GTAAGTA702.7015325E-522.499082
GCCCACG2150.021.97753118
CCCACGA2150.021.97584519
ATGCCGT2150.021.97584544
TCCGAGC2200.021.4780413
Produced by FastQC (version 0.11.2)