FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_29.341000000049ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_29.341000000049ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583509
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1150.031.30309743
AGTCTAG450.001225504125.00114318
TATTCAA901.1888915E-824.99930
GACCTAT952.1118467E-823.68326226
ACCTATT1052.3610482E-923.57048627
AATCTCG1252.910383E-1123.39906336
GTATCAA10200.022.952061
TCTCGTA1600.022.499138
ATGCCGT1600.022.499144
CTCGTAT1650.021.81730839
ACGAGAC1650.021.81730822
CGTATGC1700.021.17562341
TGCCGTC1750.020.57060645
TAGTGAA550.003933210420.45547911
ACGGGTA550.00393516320.4537263
CTATTCA1109.9671524E-820.45372629
TCGTATG1800.019.999240
CCACGAG1950.019.614620
TACCGCA706.9716625E-419.28824813
GTGATCG706.975685E-419.2865942
Produced by FastQC (version 0.11.2)