FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_28.3410000000499f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_28.3410000000499f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731324
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.024.0716441
CTCGTAT1501.4551915E-1120.99936339
ATTGGGC654.2370698E-420.76863
TCGACAC550.00393588220.4539243
TATGCCG1603.8198777E-1119.68690343
CATCTCC26250.019.2851345
TGCCGTC1757.2759576E-1219.2851345
GCGTGCC600.00650942718.749433
GCACCGT851.20396144E-418.5288496
TGGGCCG851.20396144E-418.5288495
GTATCAA11250.018.4069921
GTACACG750.001106872518.006841
TCTCCGA7950.017.82964745
TCGTATG1802.0736479E-1017.4994740
CTACATA901.8649394E-417.499474
GTCATAT800.001705699716.8814131
AATCTCG1601.6112608E-816.87448736
ATGCCGT1904.474714E-1016.57844444
TACATAG1104.6350116E-516.363145
GAGCTAT1004.1654194E-415.7495216
Produced by FastQC (version 0.11.2)