##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 6h_27.34100000004982.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3717
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.09443099273608	38.0	32.0	38.0	27.0	38.0
2	34.450363196125906	38.0	32.0	38.0	27.0	38.0
3	34.46758138283562	38.0	32.0	38.0	32.0	38.0
4	34.88835082055421	38.0	32.0	38.0	32.0	38.0
5	34.64675813828356	38.0	32.0	38.0	32.0	38.0
6	36.25746569814366	38.0	38.0	40.0	32.0	40.0
7	36.191283292978206	38.0	38.0	40.0	32.0	40.0
8	35.96475652407856	38.0	38.0	40.0	27.0	40.0
9	36.174872208770516	38.0	38.0	40.0	32.0	40.0
10	35.91068065644337	38.0	38.0	40.0	27.0	40.0
11	36.07721280602637	38.0	38.0	40.0	32.0	40.0
12	35.912563895614745	38.0	38.0	40.0	27.0	40.0
13	35.848533763788	38.0	38.0	40.0	27.0	40.0
14	35.72370191014259	38.0	38.0	40.0	27.0	40.0
15	35.64272262577347	38.0	38.0	40.0	27.0	40.0
16	35.69787463007802	38.0	38.0	40.0	27.0	40.0
17	35.51708366962604	38.0	32.0	40.0	27.0	40.0
18	35.813828356201235	38.0	38.0	40.0	27.0	40.0
19	35.653483992467045	38.0	38.0	40.0	27.0	40.0
20	35.675813828356205	38.0	38.0	40.0	27.0	40.0
21	35.74603174603175	38.0	32.0	40.0	27.0	40.0
22	35.84315308044122	38.0	38.0	40.0	27.0	40.0
23	35.78262039278989	38.0	38.0	40.0	27.0	40.0
24	35.68926553672316	38.0	38.0	40.0	27.0	40.0
25	35.53241861716438	38.0	32.0	40.0	27.0	40.0
26	35.36507936507937	38.0	32.0	40.0	27.0	40.0
27	35.50659133709981	38.0	38.0	40.0	27.0	40.0
28	35.56174334140436	38.0	38.0	40.0	27.0	40.0
29	35.49475383373689	38.0	38.0	40.0	27.0	40.0
30	35.33414043583535	38.0	32.0	40.0	27.0	40.0
31	35.39978477266613	38.0	32.0	40.0	27.0	40.0
32	35.40382028517622	38.0	32.0	40.0	27.0	40.0
33	35.46919558783966	38.0	32.0	40.0	27.0	40.0
34	35.50417002959376	38.0	38.0	40.0	27.0	40.0
35	35.37019101425881	38.0	32.0	40.0	27.0	40.0
36	35.37126715092817	38.0	32.0	40.0	27.0	40.0
37	35.33575464083939	38.0	32.0	40.0	27.0	40.0
38	35.31181059994619	38.0	32.0	40.0	27.0	40.0
39	35.0938929244014	38.0	32.0	40.0	27.0	40.0
40	35.17621737960721	38.0	32.0	40.0	27.0	40.0
41	35.2378262039279	38.0	32.0	40.0	27.0	40.0
42	35.3153080441216	38.0	32.0	40.0	27.0	40.0
43	35.30508474576271	38.0	32.0	40.0	27.0	40.0
44	35.23405972558515	38.0	32.0	40.0	27.0	40.0
45	35.35243475921442	38.0	32.0	40.0	27.0	40.0
46	35.059725585149316	38.0	32.0	40.0	27.0	40.0
47	35.08366962604251	38.0	32.0	40.0	27.0	40.0
48	34.99327414581652	38.0	32.0	40.0	27.0	40.0
49	34.909604519774014	38.0	32.0	40.0	27.0	40.0
50	34.96341135324186	38.0	32.0	40.0	27.0	40.0
51	34.364272262577344	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	4.113876488095229
2201	2	-0.08805803571428328
2201	3	3.6529389880952294
2201	4	2.779780505952381
2201	5	1.2426339285714363
2201	6	2.3468377976190595
2201	7	3.2449776785714306
2201	8	3.6085565476190453
2201	9	-2.4654203869047535
2201	10	0.8848214285714349
2201	11	-3.4294828869047578
2201	12	-2.0577566964285694
2201	13	-2.41822916666667
2201	14	-2.6191220238095028
2201	15	2.626953125000014
2201	16	-0.6133928571428697
2201	17	0.9509858630952479
2201	18	2.644568452380959
2201	19	-1.6695870535714334
2201	20	0.05249255952381304
2201	21	1.4395089285714349
2201	22	-2.001506696428578
2201	23	0.42323288690475636
2201	24	0.949944196428568
2201	25	1.7848214285714263
2201	26	-1.0562499999999986
2201	27	-1.1460007440476119
2201	28	-1.3172433035714235
2201	29	2.919642857142847
2201	30	-0.7830171130952266
2201	31	1.1275297619047748
2201	32	1.3092819940476303
2201	33	0.2716703869047592
2201	34	1.2730282738095156
2201	35	1.5518601190476247
2201	36	0.9962797619047663
2201	37	1.0380580357142648
2201	38	3.771744791666663
2201	39	-1.4500558035714306
2201	40	1.7286272321428555
2201	41	1.0721354166666686
2201	42	-3.5038504464285545
2201	43	-2.288076636904755
2201	44	-1.7005952380952394
2201	45	0.4159226190476204
2201	46	-0.3272879464285765
2201	47	-1.3772693452380906
2201	48	-7.430412946428575
2201	49	-4.1614211309523945
2201	50	-2.991443452380956
2201	51	1.6690848214285694
1101	1	4.113876488095229
1101	2	3.511941964285718
1101	3	1.2243675595237988
1101	4	1.0654947916666657
1101	5	2.7854910714285808
1101	6	2.6896949404762083
1101	7	2.902120535714282
1101	8	2.865699404761898
1101	9	2.8774367559523952
1101	10	3.3419642857142904
1101	11	2.2562313988095255
1101	12	2.427957589285718
1101	13	1.8960565476190467
1101	14	1.2665922619047834
1101	15	-1.058761160714269
1101	16	2.815178571428561
1101	17	-0.9347284226190382
1101	18	-2.441145833333323
1101	19	-1.2981584821428669
1101	20	-0.5760788690476204
1101	21	3.5823660714285808
1101	22	-1.6586495535714292
1101	23	-0.9481956845238102
1101	24	2.03565848214285
1101	25	-2.786607142857143
1101	26	-1.9133928571428527
1101	27	0.6539992559523853
1101	28	1.6256138392857196
1101	29	2.805357142857133
1101	30	-0.15444568452379315
1101	31	0.6132440476190624
1101	32	0.5092819940476332
1101	33	-6.899758184523815
1101	34	2.044456845238088
1101	35	3.6375744047619065
1101	36	-0.48943452380952124
1101	37	-1.7333705357143074
1101	38	0.5431733630952351
1101	39	1.1785156250000028
1101	40	-4.04280133928572
1101	41	1.7292782738095198
1101	42	0.010435267857161534
1101	43	3.4547805059523924
1101	44	4.4422619047619065
1101	45	1.787351190476187
1101	46	2.6727120535714235
1101	47	3.3941592261904816
1101	48	3.426729910714279
1101	49	0.2100074404761756
1101	50	-1.191443452380959
1101	51	1.040513392857136
2202	1	2.9138764880952266
2202	2	1.3119419642857153
2202	3	0.25293898809523085
2202	4	1.5797805059523782
2202	5	2.442633928571432
2202	6	-3.4531622023809376
2202	7	-1.155022321428575
2202	8	-4.5914434523809575
2202	9	-2.4654203869047535
2202	10	-0.5151785714285637
2202	11	2.3705171130952394
2202	12	-3.6577566964285637
2202	13	-7.41822916666667
2202	14	-1.2191220238095042
2202	15	-2.5730468749999815
2202	16	-4.013392857142868
2202	17	0.3509858630952465
2202	18	1.0445684523809646
2202	19	2.1304129464285637
2202	20	2.6524925595238074
2202	21	0.8395089285714334
2202	22	0.7984933035714263
2202	23	-13.57676711309524
2202	24	-4.450055803571434
2202	25	0.9848214285714292
2202	26	1.34375
2202	27	2.2539992559523867
2202	28	0.48275669642857366
2202	29	-7.480357142857152
2202	30	-3.783017113095223
2202	31	-1.6724702380952223
2202	32	0.30928199404763035
2202	33	-0.3283296130952422
2202	34	-1.7269717261904844
2202	35	-1.6481398809523782
2202	36	3.9962797619047663
2202	37	-1.5619419642857366
2202	38	-6.628255208333336
2202	39	-1.2500558035714278
2202	40	-3.871372767857146
2202	41	-2.9278645833333314
2202	42	2.496149553571449
2202	43	-3.4880766369047507
2202	44	-2.9005952380952387
2202	45	1.4159226190476204
2202	46	0.47271205357142065
2202	47	-1.7772693452380892
2202	48	-0.4304129464285751
2202	49	0.6385788690476062
2202	50	3.2085565476190396
2202	51	0.06908482142856798
1102	1	2.399590773809514
1102	2	0.22622767857143344
1102	3	2.08151041666666
1102	4	1.0654947916666657
1102	5	-0.6430803571428498
1102	6	2.4039806547619165
1102	7	1.4735491071428584
1102	8	1.1514136904761898
1102	9	1.7345796130952493
1102	10	0.6276785714285822
1102	11	0.6848028273809561
1102	12	-0.5720424107142819
1102	13	3.0389136904761855
1102	14	1.4094494047619293
1102	15	3.0840959821428697
1102	16	1.3866071428571303
1102	17	2.065271577380962
1102	18	1.558854166666677
1102	19	2.1304129464285637
1102	20	1.8524925595238102
1102	21	3.0109375000000043
1102	22	1.6270647321428555
1102	23	-0.9481956845238102
1102	24	2.749944196428565
1102	25	-0.21517857142857366
1102	26	2.6580357142857167
1102	27	-5.488857886904757
1102	28	0.9113281250000043
1102	29	3.3767857142857025
1102	30	1.702697172619061
1102	31	2.1846726190476318
1102	32	1.9378534226190638
1102	33	2.385956101190473
1102	34	2.4730282738095184
1102	35	3.066145833333337
1102	36	-3.2037202380952365
1102	37	-0.5905133928571686
1102	38	2.2574590773809504
1102	39	1.3213727678571416
1102	40	1.6714843749999986
1102	41	-0.9850074404761884
1102	42	1.581863839285731
1102	43	0.3119233630952465
1102	44	-0.2720238095238088
1102	45	-0.49836309523809774
1102	46	2.101283482142854
1102	47	2.251302083333343
1102	48	3.426729910714279
1102	49	2.3528645833333215
1102	50	2.2371279761904717
1102	51	1.6119419642857125
2203	1	-3.286123511904769
2203	2	0.11194196428571956
2203	3	3.6529389880952294
2203	4	1.5797805059523782
2203	5	2.442633928571432
2203	6	0.1468377976190638
2203	7	1.6449776785714292
2203	8	0.20855654761904674
2203	9	0.33457961309525075
2203	10	-1.7151785714285666
2203	11	-2.229482886904762
2203	12	0.7422433035714349
2203	13	3.3817708333333343
2203	14	1.1808779761905015
2203	15	-1.9730468749999872
2203	16	-2.8133928571428726
2203	17	-1.8490141369047564
2203	18	2.644568452380959
2203	19	3.3304129464285666
2203	20	2.6524925595238074
2203	21	1.6395089285714377
2203	22	3.7984933035714263
2203	23	3.823232886904762
2203	24	1.949944196428568
2203	25	-2.2151785714285737
2203	26	-0.8562499999999957
2203	27	2.6539992559523853
2203	28	3.282756696428571
2203	29	-1.2803571428571487
2203	30	3.4169828869047763
2203	31	4.127529761904775
2203	32	2.1092819940476346
2203	33	2.271670386904759
2203	34	3.6730282738095212
2203	35	-6.648139880952378
2203	36	-1.803720238095238
2203	37	1.838058035714262
2203	38	2.571744791666667
2203	39	3.5499441964285694
2203	40	1.7286272321428555
2203	41	-6.327864583333334
2203	42	0.09614955357144339
2203	43	0.9119233630952479
2203	44	-0.9005952380952422
2203	45	1.4159226190476204
2203	46	-1.1272879464285737
2203	47	2.022730654761915
2203	48	2.569587053571425
2203	49	2.2385788690476076
2203	50	-1.3914434523809618
2203	51	-4.730915178571433
1103	1	3.1138764880952294
1103	2	0.6786086309523824
1103	3	-1.1803943452381063
1103	4	-4.220219494047619
1103	5	-0.35736607142856514
1103	6	-2.2864955357142733
1103	7	-0.12168898809523654
1103	8	0.3418898809523796
1103	9	-3.432087053571415
1103	10	-2.681845238095228
1103	11	-1.0294828869047592
1103	12	0.6422433035714334
1103	13	1.5151041666666671
1103	14	-3.852455357142837
1103	15	-1.60638020833332
1103	16	0.05327380952379457
1103	17	1.1843191964285822
1103	18	-1.6554315476190382
1103	19	1.797079613095228
1103	20	1.5191592261904745
1103	21	1.7728422619047706
1103	22	2.198493303571425
1103	23	2.2898995535714235
1103	24	1.4166108630952294
1103	25	1.4514880952380906
1103	26	-0.8895833333333343
1103	27	1.1539992559523853
1103	28	3.149423363095238
1103	29	2.1863095238095127
1103	30	3.0836495535714405
1103	31	1.994196428571442
1103	32	1.1759486607142975
1103	33	2.6716703869047578
1103	34	-0.6936383928571459
1103	35	2.1851934523809575
1103	36	-1.7037202380952365
1103	37	2.5047247023809263
1103	38	3.63841145833333
1103	39	1.4166108630952365
1103	40	3.195293898809517
1103	41	2.8721354166666657
1103	42	-0.8705171130952181
1103	43	0.9785900297619108
1103	44	3.966071428571425
1103	45	1.8825892857142819
1103	46	-0.4939546130952408
1103	47	3.4893973214285765
1103	48	3.236253720238089
1103	49	0.1385788690476062
1103	50	-4.858110119047627
1103	51	0.46908482142856656
2204	1	1.2805431547618937
2204	2	-0.48805803571428186
2204	3	-0.34706101190477057
2204	4	0.9464471726190453
2204	5	0.8093005952380992
2204	6	0.8801711309523981
2204	7	0.8783110119047635
2204	8	1.3418898809523796
2204	9	-2.7654203869047507
2204	10	-0.5151785714285637
2204	11	0.3038504464285765
2204	12	0.14224330357143344
2204	13	0.6817708333333314
2204	14	-3.1857886904761656
2204	15	1.89361979166668
2204	16	2.3866071428571303
2204	17	2.684319196428582
2204	18	2.1779017857142975
2204	19	-3.0362537202381006
2204	20	1.8524925595238102
2204	21	0.7728422619047706
2204	22	-1.6348400297619108
2204	23	0.7898995535714235
2204	24	-4.750055803571435
2204	25	0.28482142857142634
2204	26	1.2770833333333371
2204	27	2.3206659226190496
2204	28	3.149423363095238
2204	29	1.8529761904761841
2204	30	2.5836495535714405
2204	31	-3.6724702380952223
2204	32	0.00928199404763319
2204	33	-1.161662946428578
2204	34	1.806361607142854
2204	35	2.6851934523809575
2204	36	1.7962797619047635
2204	37	3.1713913690475977
2204	38	3.63841145833333
2204	39	0.9166108630952365
2204	40	1.0286272321428527
2204	41	1.7054687500000014
2204	42	2.2961495535714462
2204	43	1.9785900297619108
2204	44	2.966071428571425
2204	45	2.882589285714282
2204	46	2.339378720238088
2204	47	0.8227306547619122
2204	48	-1.2637462797619108
2204	49	-5.361421130952394
2204	50	0.14188988095237676
2204	51	-2.1975818452380977
2205	1	1.7138764880952309
2205	2	3.511941964285718
2205	3	1.2529389880952309
2205	4	1.5797805059523782
2205	5	1.2426339285714363
2205	6	2.746837797619065
2205	7	3.2449776785714306
2205	8	2.008556547619044
2205	9	2.934579613095252
2205	10	-1.7151785714285666
2205	11	0.770517113095238
2205	12	3.3422433035714363
2205	13	3.3817708333333343
2205	14	3.1808779761905015
2205	15	3.4269531250000185
2205	16	2.586607142857133
2205	17	4.3509858630952465
2205	18	1.4445684523809632
2205	19	0.9304129464285609
2205	20	0.8524925595238102
2205	21	2.439508928571435
2205	22	-1.6015066964285722
2205	23	3.823232886904762
2205	24	3.949944196428568
2205	25	-2.6151785714285722
2205	26	-0.8562499999999957
2205	27	1.2539992559523867
2205	28	2.4827566964285737
2205	29	-1.2803571428571487
2205	30	-1.9830171130952223
2205	31	-0.8724702380952252
2205	32	3.3092819940476303
2205	33	3.471670386904755
2205	34	2.07302827380952
2205	35	-0.44813988095237534
2205	36	2.396279761904765
2205	37	4.038058035714265
2205	38	2.3717447916666643
2205	39	3.5499441964285694
2205	40	-0.8713727678571459
2205	41	-0.5278645833333329
2205	42	4.496149553571449
2205	43	2.3119233630952465
2205	44	-0.9005952380952422
2205	45	-1.5840773809523796
2205	46	1.6727120535714235
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1211	23	2.623232886904759
1211	24	1.7499441964285651
1211	25	2.7848214285714263
1211	26	2.6104166666666657
1211	27	2.3206659226190496
1211	28	3.4827566964285737
1211	29	3.1863095238095127
1211	30	3.0836495535714405
1211	31	3.994196428571442
1211	32	1.342615327380969
1211	33	2.6716703869047578
1211	34	3.4730282738095184
1211	35	4.6851934523809575
1211	36	3.4629464285714278
1211	37	3.5047247023809263
1211	38	3.9717447916666657
1211	39	3.083277529761908
1211	40	3.5286272321428527
1211	41	3.2054687500000014
1211	42	2.4628162202381105
1211	43	2.978590029761911
1211	44	2.966071428571425
1211	45	3.2159226190476176
1211	46	3.6727120535714235
1211	47	2.822730654761912
1211	48	3.9029203869047606
1211	49	3.3052455357142705
1211	50	3.1418898809523768
1211	51	4.469084821428567
1208	1	-5.219456845238103
1208	2	-0.32139136904761756
1208	3	0.8196056547618937
1208	4	0.779780505952381
1208	5	2.642633928571435
1208	6	2.880171130952398
1208	7	3.0449776785714278
1208	8	3.008556547619044
1208	9	2.7345796130952493
1208	10	-0.3485119047618994
1208	11	1.637183779761905
1208	12	-5.857756696428567
1208	13	-1.3182291666666686
1208	14	1.9808779761904987
1208	15	3.2269531250000156
1208	16	2.719940476190466
1208	17	3.3509858630952465
1208	18	2.1779017857142975
1208	19	3.1304129464285637
1208	20	1.185825892857146
1208	21	2.7728422619047706
1208	22	3.198493303571425
1208	23	2.623232886904759
1208	24	3.4166108630952294
1208	25	3.7848214285714263
1208	26	2.9437500000000014
1208	27	-2.6793340773809504
1208	28	-2.850576636904762
1208	29	0.3529761904761841
1208	30	-1.0830171130952237
1208	31	1.8275297619047777
1208	32	3.509281994047633
1208	33	-0.4949962797619065
1208	34	1.306361607142854
1208	35	1.8518601190476218
1208	36	2.7962797619047635
1208	37	2.5047247023809263
1208	38	1.9717447916666657
1208	39	1.7499441964285722
1208	40	2.5286272321428527
1208	41	1.8721354166666657
1208	42	2.4628162202381105
1208	43	1.9785900297619108
1208	44	1.7994047619047606
1208	45	3.2159226190476176
1208	46	2.6727120535714235
1208	47	3.822730654761912
1208	48	2.569587053571425
1208	49	3.638578869047606
1208	50	2.808556547619041
1208	51	4.469084821428567
1209	1	4.113876488095229
1209	2	0.5119419642857181
1209	3	3.6529389880952294
1209	4	-0.22021949404761898
1209	5	2.142633928571435
1209	6	1.0468377976190624
1209	7	3.5449776785714278
1209	8	2.008556547619044
1209	9	2.7345796130952493
1209	10	-3.2651785714285637
1209	11	-0.2794828869047592
1209	12	2.1422433035714334
1209	13	1.6817708333333314
1209	14	2.9808779761904987
1209	15	0.47695312500001563
1209	16	-0.36339285714286973
1209	17	-2.8990141369047535
1209	18	1.3445684523809618
1209	19	2.6304129464285637
1209	20	-0.1475074404761898
1209	21	-2.810491071428565
1209	22	3.198493303571425
1209	23	3.123232886904759
1209	24	0.7499441964285651
1209	25	2.2848214285714263
1209	26	3.4437500000000014
1209	27	4.153999255952385
1209	28	3.9827566964285737
1209	29	-2.7303571428571516
1209	30	0.6669828869047763
1209	31	1.0775297619047777
1209	32	-3.740718005952367
1209	33	-3.5783296130952422
1209	34	-1.2769717261904816
1209	35	-0.8981398809523782
1209	36	-7.7037202380952365
1209	37	0.08805803571426196
1209	38	2.9717447916666657
1209	39	2.7499441964285722
1209	40	-3.7213727678571473
1209	41	1.3721354166666657
1209	42	-0.45385044642855377
1209	43	2.3119233630952465
1209	44	2.2994047619047606
1209	45	1.2159226190476176
1209	46	1.6727120535714235
1209	47	3.322730654761912
1209	48	1.569587053571425
1209	49	3.138578869047606
1209	50	3.808556547619041
1209	51	4.469084821428567
1214	1	3.1138764880952294
1214	2	-0.32139136904761756
1214	3	2.6529389880952294
1214	4	1.779780505952381
1214	5	-0.35736607142856514
1214	6	2.2135044642857267
1214	7	1.711644345238092
1214	8	3.008556547619044
1214	9	1.7345796130952493
1214	10	-2.0151785714285637
1214	11	-1.862816220238095
1214	12	-1.6910900297619023
1214	13	1.3484374999999957
1214	14	-9.852455357142833
1214	15	-1.2730468749999844
1214	16	1.719940476190466
1214	17	3.3509858630952465
1214	18	1.511235119047626
1214	19	2.797079613095228
1214	20	1.5191592261904745
1214	21	2.106175595238099
1214	22	0.5318266369047606
1214	23	1.1232328869047592
1214	24	1.5832775297619008
1214	25	2.7848214285714263
1214	26	2.6104166666666657
1214	27	3.3206659226190496
1214	28	-1.350576636904762
1214	29	1.8529761904761841
1214	30	-0.08301711309522375
1214	31	0.49419642857144197
1214	32	1.5092819940476332
1214	33	2.6716703869047578
1214	34	2.1396949404761827
1214	35	2.6851934523809575
1214	36	1.1296130952380992
1214	37	2.838058035714262
1214	38	1.9717447916666657
1214	39	2.7499441964285722
1214	40	2.5286272321428527
1214	41	2.2054687500000014
1214	42	-2.5371837797618895
1214	43	0.8119233630952465
1214	44	2.1327380952380963
1214	45	3.2159226190476176
1214	46	4.006045386904759
1214	47	4.156063988095248
1214	48	2.9029203869047606
1214	49	1.6385788690476062
1214	50	1.6418898809523768
1214	51	1.8024181547619023
1215	1	-4.486123511904772
1215	2	2.3119419642857153
1215	3	2.4529389880952266
1215	4	1.5797805059523782
1215	5	-4.957366071428567
1215	6	-1.4531622023809376
1215	7	-2.5550223214285737
1215	8	2.4085565476190425
1215	9	0.9345796130952522
1215	10	1.0848214285714377
1215	11	2.3705171130952394
1215	12	2.542243303571432
1215	13	0.9817708333333286
1215	14	-4.819122023809502
1215	15	-1.5730468749999815
1215	16	-1.8133928571428726
1215	17	1.950985863095248
1215	18	2.644568452380959
1215	19	-2.8695870535714363
1215	20	-2.747507440476191
1215	21	-3.3604910714285623
1215	22	-2.801506696428575
1215	23	-1.3767671130952408
1215	24	-3.050055803571432
1215	25	-1.815178571428575
1215	26	-3.256249999999998
1215	27	0.05399925595238386
1215	28	-4.317243303571427
1215	29	-3.2803571428571487
1215	30	-2.383017113095221
1215	31	2.927529761904779
1215	32	2.1092819940476346
1215	33	3.471670386904755
1215	34	0.27302827380951555
1215	35	-1.2481398809523796
1215	36	0.7962797619047635
1215	37	2.838058035714262
1215	38	3.3717447916666643
1215	39	3.149944196428571
1215	40	3.32862723214285
1215	41	2.472135416666667
1215	42	2.8961495535714477
1215	43	1.911923363095248
1215	44	2.0994047619047578
1215	45	-3.5840773809523796
1215	46	3.072712053571422
1215	47	0.4227306547619136
1215	48	-4.230412946428576
1215	49	2.2385788690476076
1215	50	0.4085565476190425
1215	51	-2.3309151785714306
1212	1	-2.08612351190477
1212	2	0.11194196428571956
1212	3	-0.947061011904772
1212	4	1.5797805059523782
1212	5	-3.7573660714285637
1212	6	-2.253162202380935
1212	7	1.0449776785714278
1212	8	-2.991443452380956
1212	9	-3.6654203869047493
1212	10	1.0848214285714377
1212	11	0.17051711309524364
1212	12	0.7422433035714349
1212	13	-2.8182291666666686
1212	14	0.1808779761905015
1212	15	-3.173046874999983
1212	16	-0.21339285714287115
1212	17	-0.6490141369047535
1212	18	-0.7554315476190396
1212	19	-2.069587053571439
1212	20	-3.3475074404761926
1212	21	-7.960491071428564
1212	22	-4.201506696428574
1212	23	1.2232328869047606
1212	24	1.1499441964285637
1212	25	0.18482142857142492
1212	26	2.543750000000003
1212	27	-3.7460007440476133
1212	28	-2.5172433035714263
1212	29	-2.080357142857153
1212	30	-2.383017113095221
1212	31	-1.072470238095221
1212	32	-1.290718005952364
1212	33	-0.7283296130952408
1212	34	-2.92697172619048
1212	35	1.9518601190476232
1212	36	-2.2037202380952365
1212	37	-1.3619419642857409
1212	38	0.9717447916666657
1212	39	-0.8500558035714292
1212	40	-0.07137276785714874
1212	41	1.4721354166666671
1212	42	-2.1038504464285523
1212	43	1.911923363095248
1212	44	-2.100595238095238
1212	45	-4.184077380952381
1212	46	0.07271205357142208
1212	47	-3.5772693452380864
1212	48	-3.8304129464285737
1212	49	-0.9614211309523952
1212	50	-1.7914434523809604
1212	51	-3.330915178571434
1213	1	2.1138764880952294
1213	2	-4.821391369047614
1213	3	3.6529389880952294
1213	4	-4.886886160714287
1213	5	-6.690699404761897
1213	6	-7.78649553571427
1213	7	-11.955022321428572
1213	8	-1.6581101190476204
1213	9	-2.2654203869047507
1213	10	-5.848511904761896
1213	11	-10.362816220238091
1213	12	0.14224330357143344
1213	13	-1.8182291666666686
1213	14	-8.352455357142833
1213	15	-1.7730468749999844
1213	16	-2.6133928571428697
1213	17	-7.982347470238086
1213	18	-6.48876488095237
1213	19	2.1304129464285637
1213	20	1.8524925595238102
1213	21	-3.560491071428565
1213	22	-10.134840029761907
1213	23	-3.043433779761905
1213	24	-2.916722470238099
1213	25	-1.2151785714285737
1213	26	0.9437500000000014
1213	27	-17.679334077380947
1213	28	-16.183909970238094
1213	29	-9.813690476190484
1213	30	-21.583017113095224
1213	31	-7.0058035714285545
1213	32	-9.490718005952367
1213	33	0.6716703869047578
1213	34	-3.193638392857146
1213	35	-6.98147321428571
1213	36	2.7962797619047635
1213	37	-2.8286086309524023
1213	38	2.9717447916666657
1213	39	0.7499441964285722
1213	40	-5.8047061011904795
1213	41	-4.461197916666666
1213	42	-8.703850446428554
1213	43	-3.354743303571418
1213	44	-9.033928571428572
1213	45	-3.4507440476190467
1213	46	-1.3272879464285765
1213	47	0.8227306547619122
1213	48	-9.430412946428575
1213	49	-1.028087797619058
1213	50	2.808556547619041
1213	51	-6.864248511904766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	1.0
22	4.0
23	11.0
24	19.0
25	44.0
26	65.0
27	73.0
28	87.0
29	127.0
30	154.0
31	156.0
32	204.0
33	219.0
34	229.0
35	251.0
36	351.0
37	440.0
38	604.0
39	676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.17653390742734	15.33907427341227	13.293864370290635	37.19052744886975
2	13.882163034705409	21.603443637341943	39.76324993274146	24.75114339521119
3	16.626311541565777	23.405972558514932	33.70998116760829	26.257734732311004
4	12.967446865751949	21.791767554479417	33.037395749260156	32.20338983050847
5	13.882163034705409	33.49475383373689	38.660209846650524	13.962873284907182
6	29.88969599139091	40.16680118375034	16.249663707290825	13.693839117567933
7	24.83185364541297	32.52623083131558	19.235942964756525	23.405972558514932
8	22.00699488835082	41.162227602905574	18.401937046004843	18.42884046273877
9	25.20850147968792	16.545601291364004	19.397363465160076	38.848533763788
10	15.119720204465967	32.39171374764595	30.293247242399783	22.1953188054883
11	31.692224912563894	22.814097390368577	26.580575733118106	18.913101963949423
12	21.011568469195588	25.692762980898575	27.656712402475115	25.638956147430726
13	27.656712402475115	20.823244552058114	29.351627656712402	22.168415388754372
14	21.33440947000269	20.09685230024213	25.854183481302123	32.714554748453054
15	23.056228140973904	27.280064568200164	22.383642722625773	27.280064568200164
16	26.096314231907453	27.602905569007262	24.239978477266614	22.06080172181867
17	21.71105730427764	32.36481033091202	24.051654560129137	21.872477804681196
18	27.979553403282214	26.0425073984396	25.181598062954	20.796341135324187
19	24.724239978477268	25.289211729889693	23.70191014258811	26.28463814904493
20	22.114608555286523	26.446058649448478	24.266881894000537	27.17245090126446
21	21.84557438794727	25.289211729889693	24.509012644605864	28.356201237557173
22	22.867904223836426	29.674468657519505	25.262308313155774	22.1953188054883
23	21.872477804681196	24.670433145009415	24.724239978477268	28.732849071832124
24	27.683615819209038	25.181598062954	24.64352972827549	22.491256389561475
25	22.545063223029324	31.019639494215767	25.343018563357546	21.092278719397363
26	26.39225181598063	25.6120527306968	25.343018563357546	22.652676889965026
27	21.253699219800914	31.127253161151465	26.768899650255584	20.850147968792037
28	21.038471885929514	26.634382566585955	24.858757062146893	27.468388485337634
29	24.42830239440409	27.468388485337634	26.17702448210923	21.926284638149045
30	23.486682808716708	26.93032015065913	26.28463814904493	23.29835889157923
31	22.168415388754372	25.04708097928437	27.199354317998388	25.585149313962873
32	23.271455474845304	25.638956147430726	26.338444982512776	24.75114339521119
33	21.737960721011568	25.31611514662362	29.136400322841	23.809523809523807
34	24.509012644605864	25.073984396018297	27.17245090126446	23.24455205811138
35	23.997847726661288	24.401398977670162	27.253161151466237	24.347592144202313
36	21.818670971213344	25.181598062954	30.750605326876514	22.249125638956148
37	21.388216303470543	26.68818940005381	27.710519235942964	24.213075060532688
38	19.827818132902877	21.576540220608017	34.00591875168146	24.589722894807643
39	19.693301049233252	21.388216303470543	29.701372074253428	29.217110573042778
40	19.639494215765403	21.19989238633306	35.56631692224912	23.594296475652406
41	19.908528383104652	21.522733387140168	29.405434490180255	29.163303739574925
42	23.86333064299166	21.49582997040624	31.34248049502287	23.29835889157923
43	18.052192628463814	20.688727468388485	36.13128867366156	25.127791229486146
44	15.49636803874092	27.011030400860907	33.33333333333333	24.159268227064835
45	16.06133979015335	22.760290556900724	35.40489642184557	25.773473231100354
46	20.392789884315306	22.00699488835082	33.46785041700296	24.132364810330913
47	14.312617702448211	23.271455474845304	31.719128329297817	30.69679849340866
48	13.774549367769707	23.45977939198278	31.288673661555016	31.476997578692494
49	18.267419962335214	22.679580306698952	32.3379069141781	26.715092816787735
50	13.155770782889428	24.37449556093624	35.59322033898305	26.876513317191282
51	13.693839117567933	23.75571697605596	30.239440408931934	32.311003497444176
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.0
15	3.0
16	3.0
17	2.5
18	2.0
19	1.5
20	1.0
21	1.5
22	2.0
23	1.5
24	1.0
25	2.5
26	6.5
27	9.0
28	9.0
29	9.0
30	19.5
31	30.0
32	54.0
33	78.0
34	101.0
35	124.0
36	147.5
37	171.0
38	191.5
39	212.0
40	241.5
41	271.0
42	329.5
43	388.0
44	388.0
45	388.0
46	433.0
47	478.0
48	437.5
49	397.0
50	392.0
51	387.0
52	341.5
53	296.0
54	237.5
55	179.0
56	141.0
57	103.0
58	84.0
59	65.0
60	53.0
61	41.0
62	30.0
63	19.0
64	15.0
65	11.0
66	16.0
67	21.0
68	14.5
69	8.0
70	5.5
71	3.0
72	3.5
73	4.0
74	3.0
75	2.5
76	3.0
77	1.5
78	0.0
79	1.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026903416733925208
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3717.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.86144740382029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.09245604083948	94.00053806833468
2	0.5104934770277936	0.9685230024213075
3	0.02836074872376631	0.08071025020177562
4	0.05672149744753262	0.21522733387140167
5	0.02836074872376631	0.13451708366962603
6	0.02836074872376631	0.16142050040355124
7	0.02836074872376631	0.18832391713747645
8	0.02836074872376631	0.21522733387140167
9	0.0	0.0
>10	0.19852524106636413	4.035512510088781
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	31	0.8340059187516815	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	25	0.6725854183481302	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	24	0.645682001614205	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	24	0.645682001614205	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	23	0.6187785848802798	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	12	0.3228410008071025	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	11	0.29593758407317733	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	8	0.21522733387140167	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	7	0.18832391713747645	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	6	0.16142050040355124	RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	5	0.13451708366962603	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	4	0.10761366693570083	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	4	0.10761366693570083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.026903416733925208
8	0.0	0.0	0.026903416733925208
9	0.0	0.0	0.026903416733925208
10	0.0	0.0	0.026903416733925208
11	0.0	0.0	0.026903416733925208
12	0.0	0.0	0.026903416733925208
13	0.0	0.0	0.026903416733925208
14	0.0	0.0	0.026903416733925208
15	0.0	0.0	0.026903416733925208
16	0.0	0.0	0.026903416733925208
17	0.0	0.0	0.026903416733925208
18	0.0	0.0	0.026903416733925208
19	0.0	0.0	0.026903416733925208
20	0.0	0.0	0.026903416733925208
21	0.0	0.0	0.053806833467850416
22	0.0	0.0	0.053806833467850416
23	0.0	0.0	0.08071025020177562
24	0.0	0.0	0.10761366693570083
25	0.0	0.0	0.16142050040355124
26	0.0	0.0	0.21522733387140167
27	0.0	0.0	0.2959375840731773
28	0.0	0.0	0.5649717514124294
29	0.0	0.0	0.9147161689534571
30	0.0	0.0	1.533494753833737
31	0.0	0.0	2.9862792574656982
32	0.0	0.0	4.735001345170836
33	0.0	0.0	6.45682001614205
34	0.0	0.0	7.990314769975787
35	0.0	0.0	9.900457358084477
36	0.0	0.0	12.18724778046812
37	0.0	0.0	14.420231369383911
38	0.0	0.0	17.137476459510356
39	0.0	0.0	20.177562550443906
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTCT	145	0.0048998715	10.862068	45
CTGTCTC	150	0.0063140932	10.5	44
>>END_MODULE