FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_26.34100000004975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_26.34100000004975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474073
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC5340.11264088020199421RNA PCR Primer, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACG250.002106032335.999418
TATGCCG1400.035.35656743
ACCTATT900.034.99942427
TCGTATG1550.031.93496140
CACCGTT300.00514465629.999517
GCCCACG1700.029.11716818
CTCGTAT1700.029.11716839
ATGCCGT1700.029.11716844
GACCTAT1100.028.63589526
AATCTCG1200.028.1245436
ACGAGAC1850.027.97251522
CCCACGA1800.027.49954819
CCACGAG1950.026.53802720
TGCCGTC1900.026.05495345
TAATTGT450.001225608924.999594
TACCGTG450.001225608924.999597
GGTATCA4100.024.1612361
TCTCGTA2050.024.14594538
CGAGCCC2050.024.14594515
CTATTCA1405.456968E-1222.49963229
Produced by FastQC (version 0.11.2)