FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_25.3410000000495c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_25.3410000000495c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238339
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA6290.26390980913740514No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5840.2450291391673205No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC5660.23747687117928665RNA PCR Primer, Index 13 (95% over 21bp)
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4670.19593939724510046No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4430.18586970659438867No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4260.17873700905013445No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC4060.17034560017454128No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3660.15356278242335497No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG3290.1380386760035076No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3160.13258426023437206No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG2980.12503199224633818No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA2800.11747972425830434No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG2520.10573175183247391No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2440.10237518828223664No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACA2410.10111647695089766No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAGT250.002103765536.0000044
TGCCGTC950.033.15789445
TATGCCG1050.030.043
AATCTCG751.6898412E-930.036
ATGCCGT1050.030.044
CCACGAG1050.030.020
TACCTGG300.005139169729.99999821
ACCTATT702.8659997E-828.92857427
GACCTAT702.8659997E-828.92857426
CTCGTAT1100.028.63636439
ACTGTGC406.1516947E-428.1258
TGTAAGT856.4264896E-926.470591
CGTATGC1200.026.24999841
CCCACGA1200.026.24999819
TCTCGTA1200.026.24999838
CTATTCA801.0464828E-725.312529
AGTCTTA450.001223702825.022
ACGGGGA551.363763E-424.54545416
CACGAGA1301.8189894E-1224.2307721
AAAAGTA5550.023.9189192
Produced by FastQC (version 0.11.2)