FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_23.34100000004932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_23.34100000004932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547005
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG601.546141E-1037.4991643
CCGCCGA250.002105431436.00248716
GGTATCA3350.030.9061341
AAGCGGT300.005143190730.00207117
CGCAATC300.005143190730.00207112
AAGGCGC300.005143190730.00207115
GTCGTAT406.15949E-428.12694415
CTCGTAT803.3869583E-928.12437239
TGCCGTC856.4665073E-926.46999745
GTATCAA7250.025.1465641
GCCCACG952.1087544E-823.68584618
TCTTGCG500.002263398122.4994987
ATGCCGT1003.6363417E-822.49949844
CGAGCCC1056.088703E-821.4300515
CCCACGA1056.094524E-821.42809319
CGTATGC1109.962423E-820.45408841
ATGTCGC550.003934450420.45408820
TATGTCG550.003934450420.45408819
GTCTTAT908.239866E-619.99955232
TCTCGTA1151.5919068E-719.56478138
Produced by FastQC (version 0.11.2)