FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_22.34100000004925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_22.34100000004925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688889
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACCTGTCTCTTATACACA9270.13456449442508153No Hit
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATAC9050.13137094655307313No Hit
CTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACACTGTCTCTTATAC8670.12585481841051316No Hit
TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATACAC8180.11874191633194897No Hit
CTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACTGTCTCTTATACAC8020.1164193360613974No Hit
GTTCAAATCCCAGCAACCACATGGTGGCTCACAACCTGTCTCTTATACACA7730.1122096593210227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.039.140031
ATATGTA2100.035.3658141
TATGTAA2400.031.8735622
GTACGTC300.005143326230.00300224
GTATCAA11850.028.4879971
CAATTAT709.278592E-725.7205921
GTGAGTA450.001224304225.006131
ATTAGCC450.001226378724.998873
TCGCTTG851.8921855E-723.8224539
AATTATT952.1133019E-823.683142
CATCTCC26450.023.13863245
ATTGTCG500.002263334622.50061813
ACTTACC2200.022.49898532
CTCGCTT1003.6407073E-822.4989858
ATTATTA802.9730509E-622.4989833
TGTAAGT10350.022.3967951
ATCTCGC955.5274904E-721.3148276
TACTTAC13350.021.06646331
ATACTGA754.6007975E-520.9990526
TATATTG654.237146E-420.7682935
Produced by FastQC (version 0.11.2)