FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_21.34100000004918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_21.34100000004918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611757
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA250.002106678235.9988211
GGTATCA3300.031.3728661
GTAATAG300.005137998630.0088331
ACCGTCC453.4889814E-529.9990188
GTATCAA8450.028.4995121
CCCCGAA500.002263838722.49926412
TATCAAC10900.022.0864332
GCGGTGT550.00393334720.4555528
ACGGGCC550.003935209520.45387616
TCAACGC11650.020.2783074
AACGCAG12000.019.874356
CAACGCA12050.019.7918835
TTAGAAC807.557514E-519.6868553
TCTCCGA4950.019.54481545
GTATAGT706.964551E-419.2913911
ACGGGAC1757.2759576E-1219.28508416
ATCAACG12250.019.2850843
ACGCAGA12450.019.1567
ATCTCCG7850.018.62996345
CGCAGAG12950.018.4163888
Produced by FastQC (version 0.11.2)