FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_20.3410000000490b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_20.3410000000490b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427343
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT300.00512937430.0170941
GCGTCTT300.00514407629.99952911
GTATCAA8500.023.307391
GGCATAT500.00225482222.5128211
TATACTG500.002262504822.499655
TCTACAC602.4642522E-422.4996473
ATCTCCG8150.021.53340745
ACTAATC654.232676E-420.76890621
ACCACGC654.232676E-420.7689068
TCGGACT550.003932908620.45422618
GGTCCGA807.515821E-519.6987171
TCGAAGA807.542926E-519.6894972
GACTAAT706.97438E-419.28541220
AACGCAG10450.018.0858426
TTACACA1253.819914E-717.999724
CTAATCC750.001108788517.9997222
TATCAAC10700.017.8756452
CATCTCC19550.017.83859845
CTCCGAG3050.017.70464345
GTATTGG901.8541215E-417.5099721
Produced by FastQC (version 0.11.2)